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The map label for this gene is pyrG

Identifier: 18311190

GI number: 18311190

Start: 2527964

End: 2529571

Strand: Reverse

Name: pyrG

Synonym: CPE2208

Alternate gene names: 18311190

Gene position: 2529571-2527964 (Counterclockwise)

Preceding gene: 18311191

Following gene: 18311189

Centisome position: 83.44

GC content: 33.83

Gene sequence:

>1608_bases
ATGAGCAAAAATACTAAGTATGTATTTGTAACAGGTGGAGTTGTTTCTTCATTAGGAAAAGGAATAACAGCTGCTTCATT
AGGAAGATTATTAAAAAACAGAGGATTAAGTGTAAGTATTCAAAAATTTGATCCATACCTAAACATTGACCCAGGTACAA
TGAGCCCATACCAACATGGAGAAGTTTTCGTAACTGATGATGGTGCTGAAACAGACTTAGACTTAGGTCACTATGAAAGA
TTCATAGATGAAAACTTAACTCAAAACTCAAACGTTACTTCAGGTAGAGTTTACTGGTCAGTAATATCAAAAGAGAGAAA
AGGTGAATACTTAGGAGGTACTGTACAAGTTATACCTCACATAACTAATGCTATAAAAGATAGAGTACATAGAGTAGGAA
AAGAAAGAGATGTAGATGTAGTAATCACTGAGATCGGTGGAACAATAGGTGATATCGAATCATTACCTTTCTTAGAAGCT
ATAAGACAAATCAAATATGATGTAGGTAAAGAAAATGTTTGTTTCATACATGTTACATTAGTACCTTACTTAGGAAAAGC
TGGTGAGTTAAAAACAAAACCAACTCAACACTCAGTTAAAGAGTTAAGAAGTATAGGAATTCAACCAGACATAATAGTTT
GTAGATCAGAAAAAGAATTATCAGAAGATATCAAAAAGAAAATCGGATTATTCTGTAATATAGATGCAAGCGAAGTTATT
CAAAACTTAGATGCAGAGCATTTATACGCTGTTCCTCTAATGTTACACAAAGAAGGATTAGATAGATTAGTTTGTGAAAA
ATTAGGTCTTGGATGCAGAGACATAGATAATGCTGAATGGATAGACATGGTTCATAGAATAACTCACTTAACTCATACAA
CTAAGATTGCTTTAGTTGGTAAATATGTTGAGTTACATGATGCATACATATCAGTAGTTGAAGCTTTAAATCATGGTGGA
TTAAGCAATGACACTAATGTTGAAATAGAGTGGATAAATGCTGAGGATGTAACTAAAGAAAATGTTGATGAATTATTAAG
TGGAGTAGATGGAGTTCTAGTTCCAGGTGGATTCGGAGACAGAGGAGTAGAAGGAAAAATCGAAGCTATAAGATGGGCTA
GAGAAAACAAGAAACCATTCTTAGGAATATGCTTAGGAATGCAATGTGCTGTTATAGAATACGCTAGAAACGTATTAGGA
TTAGAAGGAGCTCATTCATCAGAGTTAAATCCAGAAACTCCATTCCCAGTAATCGACTTAATGCCTGAGCAAAAAGATGT
TGAGGATTTAGGTGGAACAATGAGATTAGGATTATATCCATGTAAATTAGAAGACAATACATTCTGTAAGGATGTTTATG
CTTCAGATTTAATCTACGAAAGACATAGACATAGATATGAGTTCAACAATGAGTACAGAACTCAATTAATAGAAAGCGGA
TTAACTATAGCTGGTACTTCACCAGATGGAAGATTAGTTGAGTGTGTAGAAGTTAAGGATCATCCTTGGTTCGTAGCAGT
TCAATATCATCCAGAGTTAAAATCAAGACCAAACAGACCTCACCCATTATTCGTAGGATTTGTTGGTGCTGCATTAAACA
ATAAATAA

Upstream 100 bases:

>100_bases
TAAAACAAGCAATAAAAAACCCATTCTAAATGAGGTAATTATCATTATAAATGAAATAAAGAACGATGTCAAGAATAAAT
TTACATTAGGAGGAAGAGAT

Downstream 100 bases:

>100_bases
TTTTAGAAATATTAAGGATATCTAACTTAAATGTTAGGTGTCCTTTTTTTATTTAATAATATTAGAAATAAAATCTTATA
AAAATATACTATATATAGAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSKNTKYVFVTGGVVSSLGKGITAASLGRLLKNRGLSVSIQKFDPYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYER
FIDENLTQNSNVTSGRVYWSVISKERKGEYLGGTVQVIPHITNAIKDRVHRVGKERDVDVVITEIGGTIGDIESLPFLEA
IRQIKYDVGKENVCFIHVTLVPYLGKAGELKTKPTQHSVKELRSIGIQPDIIVCRSEKELSEDIKKKIGLFCNIDASEVI
QNLDAEHLYAVPLMLHKEGLDRLVCEKLGLGCRDIDNAEWIDMVHRITHLTHTTKIALVGKYVELHDAYISVVEALNHGG
LSNDTNVEIEWINAEDVTKENVDELLSGVDGVLVPGGFGDRGVEGKIEAIRWARENKKPFLGICLGMQCAVIEYARNVLG
LEGAHSSELNPETPFPVIDLMPEQKDVEDLGGTMRLGLYPCKLEDNTFCKDVYASDLIYERHRHRYEFNNEYRTQLIESG
LTIAGTSPDGRLVECVEVKDHPWFVAVQYHPELKSRPNRPHPLFVGFVGAALNNK

Sequences:

>Translated_535_residues
MSKNTKYVFVTGGVVSSLGKGITAASLGRLLKNRGLSVSIQKFDPYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYER
FIDENLTQNSNVTSGRVYWSVISKERKGEYLGGTVQVIPHITNAIKDRVHRVGKERDVDVVITEIGGTIGDIESLPFLEA
IRQIKYDVGKENVCFIHVTLVPYLGKAGELKTKPTQHSVKELRSIGIQPDIIVCRSEKELSEDIKKKIGLFCNIDASEVI
QNLDAEHLYAVPLMLHKEGLDRLVCEKLGLGCRDIDNAEWIDMVHRITHLTHTTKIALVGKYVELHDAYISVVEALNHGG
LSNDTNVEIEWINAEDVTKENVDELLSGVDGVLVPGGFGDRGVEGKIEAIRWARENKKPFLGICLGMQCAVIEYARNVLG
LEGAHSSELNPETPFPVIDLMPEQKDVEDLGGTMRLGLYPCKLEDNTFCKDVYASDLIYERHRHRYEFNNEYRTQLIESG
LTIAGTSPDGRLVECVEVKDHPWFVAVQYHPELKSRPNRPHPLFVGFVGAALNNK
>Mature_534_residues
SKNTKYVFVTGGVVSSLGKGITAASLGRLLKNRGLSVSIQKFDPYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERF
IDENLTQNSNVTSGRVYWSVISKERKGEYLGGTVQVIPHITNAIKDRVHRVGKERDVDVVITEIGGTIGDIESLPFLEAI
RQIKYDVGKENVCFIHVTLVPYLGKAGELKTKPTQHSVKELRSIGIQPDIIVCRSEKELSEDIKKKIGLFCNIDASEVIQ
NLDAEHLYAVPLMLHKEGLDRLVCEKLGLGCRDIDNAEWIDMVHRITHLTHTTKIALVGKYVELHDAYISVVEALNHGGL
SNDTNVEIEWINAEDVTKENVDELLSGVDGVLVPGGFGDRGVEGKIEAIRWARENKKPFLGICLGMQCAVIEYARNVLGL
EGAHSSELNPETPFPVIDLMPEQKDVEDLGGTMRLGLYPCKLEDNTFCKDVYASDLIYERHRHRYEFNNEYRTQLIESGL
TIAGTSPDGRLVECVEVKDHPWFVAVQYHPELKSRPNRPHPLFVGFVGAALNNK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=47.9128856624319, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=46.1818181818182, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=46.1818181818182, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=46.1818181818182, Blast_Score=498, Evalue=1e-141,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=55.5762081784387, Blast_Score=599, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=39.5695364238411, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=44.8028673835125, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=45, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24664469, Length=552, Percent_Identity=47.2826086956522, Blast_Score=497, Evalue=1e-140,
Organism=Drosophila melanogaster, GI21357815, Length=498, Percent_Identity=45.5823293172691, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_CLOP1 (Q0TNA4)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696886.1
- ProteinModelPortal:   Q0TNA4
- SMR:   Q0TNA4
- STRING:   Q0TNA4
- GeneID:   4203868
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2472
- TIGR:   CPF_2472
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   CPER195103:CPF_2472-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59657; Mature: 59525

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 384-384 ACT_SITE 510-510 ACT_SITE 512-512

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNTKYVFVTGGVVSSLGKGITAASLGRLLKNRGLSVSIQKFDPYLNIDPGTMSPYQHG
CCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCC
EVFVTDDGAETDLDLGHYERFIDENLTQNSNVTSGRVYWSVISKERKGEYLGGTVQVIPH
EEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCCCHHHHHHH
ITNAIKDRVHRVGKERDVDVVITEIGGTIGDIESLPFLEAIRQIKYDVGKENVCFIHVTL
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEE
VPYLGKAGELKTKPTQHSVKELRSIGIQPDIIVCRSEKELSEDIKKKIGLFCNIDASEVI
ECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCEEEECCHHHHH
QNLDAEHLYAVPLMLHKEGLDRLVCEKLGLGCRDIDNAEWIDMVHRITHLTHTTKIALVG
HHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
KYVELHDAYISVVEALNHGGLSNDTNVEIEWINAEDVTKENVDELLSGVDGVLVPGGFGD
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCC
RGVEGKIEAIRWARENKKPFLGICLGMQCAVIEYARNVLGLEGAHSSELNPETPFPVIDL
CCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
MPEQKDVEDLGGTMRLGLYPCKLEDNTFCKDVYASDLIYERHRHRYEFNNEYRTQLIESG
CCCCCCHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
LTIAGTSPDGRLVECVEVKDHPWFVAVQYHPELKSRPNRPHPLFVGFVGAALNNK
CEEEECCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCCEEEEEECHHCCCC
>Mature Secondary Structure 
SKNTKYVFVTGGVVSSLGKGITAASLGRLLKNRGLSVSIQKFDPYLNIDPGTMSPYQHG
CCCCCEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCC
EVFVTDDGAETDLDLGHYERFIDENLTQNSNVTSGRVYWSVISKERKGEYLGGTVQVIPH
EEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCCCHHHHHHH
ITNAIKDRVHRVGKERDVDVVITEIGGTIGDIESLPFLEAIRQIKYDVGKENVCFIHVTL
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEE
VPYLGKAGELKTKPTQHSVKELRSIGIQPDIIVCRSEKELSEDIKKKIGLFCNIDASEVI
ECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCEEEECCHHHHH
QNLDAEHLYAVPLMLHKEGLDRLVCEKLGLGCRDIDNAEWIDMVHRITHLTHTTKIALVG
HHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
KYVELHDAYISVVEALNHGGLSNDTNVEIEWINAEDVTKENVDELLSGVDGVLVPGGFGD
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCC
RGVEGKIEAIRWARENKKPFLGICLGMQCAVIEYARNVLGLEGAHSSELNPETPFPVIDL
CCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
MPEQKDVEDLGGTMRLGLYPCKLEDNTFCKDVYASDLIYERHRHRYEFNNEYRTQLIESG
CCCCCCHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
LTIAGTSPDGRLVECVEVKDHPWFVAVQYHPELKSRPNRPHPLFVGFVGAALNNK
CEEEECCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCCEEEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA