Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 18311189

GI number: 18311189

Start: 2526259

End: 2527698

Strand: Reverse

Name: rho [H]

Synonym: CPE2207

Alternate gene names: 18311189

Gene position: 2527698-2526259 (Counterclockwise)

Preceding gene: 18311190

Following gene: 18311186

Centisome position: 83.38

GC content: 29.44

Gene sequence:

>1440_bases
TTGAATATAGAACAATATGAAAATATGACTTTAGTTCAGCTAAAAGAGCAGGCTAAAGAATTAGGAATAAAAAATATAAG
TAAAAAGAAAAAAAGCGAATTAATTGAGGAATTAAAAGCTGAATTTAATAAACAAAATAGCAATACTAAAGTAATACATA
AAGACGGTGTTATTTTAAGAGAAAAAATATCTCAAAAAGAGCAAGGTGGAAATAAAGAAAGTTTTTCTGGTGAAAGAGTA
GTTAGGAATAGCAATAATTATAATAATTCAAATGATGAAGGAAAAAAAGAGCAATTAAAGGACATGATAAGTTCATCAGA
CTCTGCTAAGGGAATACTTGAAATACTAGATAATAATAATTTTGGTTTTTTAAGATGTAGAAATTATTTAACAAGTGAAG
ATGATATATATGTTTCTCCATCACAAATAAGAAGATTTGGATTAAGAACTGGAGATGAAGTCCAAGGTAAAGTTAGAATA
CCAAAGGATGGAGAAAAGTTTAAAGCTTTACTTTATGTAGAGAGAGTAAATGGTGAAAGTCCTGAAAAAGCAGTTGGAAG
AAAGAAATTTGAAGAGTTAACACCAATATATCCAAAAGAGAGATTAAGACTTGAGACTGAAAATGGAAGAGATTTAAGTT
CAAGACTTATGGATATAATTTGTCCAATAGGTAAGGGACAAAGGGGGATGATAGTTGCACCACCTAAGGCTGGAAAAACA
ACTCTTTTAAAAAGAATAGCTCAAAACATATCAAAGAATAATCCTGAAGTTAAACTTATAGTGCTTTTAATAGATGAAAG
ACCAGAAGAAGTAACGGATATGAAGAGAAGTATAGATGGAGAAGTAATCTATTCAACTTTTGATGAAGAACCACAAAACC
ATGCTAAAGTTTCAAGTATAGTATTAGAAAGAGCTAAAAGAATGGTTGAACAAGGAAGAGATGTAGTTATCTTAATGGAT
TCTTTAACAAGATTATCAAGAGCCTATAACTTAACAGTTACTCCATCGGGAAGAACTTTATCAGGGGGACTAGATCCAGG
GGCACTTATAATGCCTAAGAAATTCTTTGGAGCAGCTAGAAATTTAGAAGAGGGCGGAAGTTTAACAATTTTAGCAACTT
CTTTAGTTGATACAGGAAGTAGAATGGATGATATGATTTTTGAAGAGTTTAAGGGTACTGGAAATATGGAAGTACACTTA
GATAGAAAGCTTCAAGAGAGAAGAATATTCCCAGCTATAGATATTTATAAATCTGGTACTAGAAAAGAAGATTTATTATT
TAATGAAGAAGAAAGAGAAGCTTCATATAAAATAAGAAGAGTATTACAAAAGGAAAATAATATAGAGGATGTAGCTGAAA
AGCTTATAAATTTATTATCAAAAACAAAAAATAATAAAGAGTTTTTACAAGTTGTTTTAAAAAGCAATTTAGAAAATTAA

Upstream 100 bases:

>100_bases
GGATATTCGAAAGTTTCTATATATAAAAGTCTTAAGGCAATCTAAAACAACTTTTCTTTACCTTATTCCACTAATTAAAA
AAAAGCAGGAGGTGCTTAAA

Downstream 100 bases:

>100_bases
ATAAGTTTTCAAAACAAAAGAAGATATTAAAGTTTATAAACTTTAATATCTTCTTTTATTATAAGAATCATTTAGCATCT
TAGTGCAAAGTTTAATTGTC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNIEQYENMTLVQLKEQAKELGIKNISKKKKSELIEELKAEFNKQNSNTKVIHKDGVILREKISQKEQGGNKESFSGERV
VRNSNNYNNSNDEGKKEQLKDMISSSDSAKGILEILDNNNFGFLRCRNYLTSEDDIYVSPSQIRRFGLRTGDEVQGKVRI
PKDGEKFKALLYVERVNGESPEKAVGRKKFEELTPIYPKERLRLETENGRDLSSRLMDIICPIGKGQRGMIVAPPKAGKT
TLLKRIAQNISKNNPEVKLIVLLIDERPEEVTDMKRSIDGEVIYSTFDEEPQNHAKVSSIVLERAKRMVEQGRDVVILMD
SLTRLSRAYNLTVTPSGRTLSGGLDPGALIMPKKFFGAARNLEEGGSLTILATSLVDTGSRMDDMIFEEFKGTGNMEVHL
DRKLQERRIFPAIDIYKSGTRKEDLLFNEEEREASYKIRRVLQKENNIEDVAEKLINLLSKTKNNKEFLQVVLKSNLEN

Sequences:

>Translated_479_residues
MNIEQYENMTLVQLKEQAKELGIKNISKKKKSELIEELKAEFNKQNSNTKVIHKDGVILREKISQKEQGGNKESFSGERV
VRNSNNYNNSNDEGKKEQLKDMISSSDSAKGILEILDNNNFGFLRCRNYLTSEDDIYVSPSQIRRFGLRTGDEVQGKVRI
PKDGEKFKALLYVERVNGESPEKAVGRKKFEELTPIYPKERLRLETENGRDLSSRLMDIICPIGKGQRGMIVAPPKAGKT
TLLKRIAQNISKNNPEVKLIVLLIDERPEEVTDMKRSIDGEVIYSTFDEEPQNHAKVSSIVLERAKRMVEQGRDVVILMD
SLTRLSRAYNLTVTPSGRTLSGGLDPGALIMPKKFFGAARNLEEGGSLTILATSLVDTGSRMDDMIFEEFKGTGNMEVHL
DRKLQERRIFPAIDIYKSGTRKEDLLFNEEEREASYKIRRVLQKENNIEDVAEKLINLLSKTKNNKEFLQVVLKSNLEN
>Mature_479_residues
MNIEQYENMTLVQLKEQAKELGIKNISKKKKSELIEELKAEFNKQNSNTKVIHKDGVILREKISQKEQGGNKESFSGERV
VRNSNNYNNSNDEGKKEQLKDMISSSDSAKGILEILDNNNFGFLRCRNYLTSEDDIYVSPSQIRRFGLRTGDEVQGKVRI
PKDGEKFKALLYVERVNGESPEKAVGRKKFEELTPIYPKERLRLETENGRDLSSRLMDIICPIGKGQRGMIVAPPKAGKT
TLLKRIAQNISKNNPEVKLIVLLIDERPEEVTDMKRSIDGEVIYSTFDEEPQNHAKVSSIVLERAKRMVEQGRDVVILMD
SLTRLSRAYNLTVTPSGRTLSGGLDPGALIMPKKFFGAARNLEEGGSLTILATSLVDTGSRMDDMIFEEFKGTGNMEVHL
DRKLQERRIFPAIDIYKSGTRKEDLLFNEEEREASYKIRRVLQKENNIEDVAEKLINLLSKTKNNKEFLQVVLKSNLEN

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=369, Percent_Identity=57.1815718157182, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 54504; Mature: 54504

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQYENMTLVQLKEQAKELGIKNISKKKKSELIEELKAEFNKQNSNTKVIHKDGVILR
CCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
EKISQKEQGGNKESFSGERVVRNSNNYNNSNDEGKKEQLKDMISSSDSAKGILEILDNNN
HHHHHHHCCCCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCC
FGFLRCRNYLTSEDDIYVSPSQIRRFGLRTGDEVQGKVRIPKDGEKFKALLYVERVNGES
EEEEEECCCCCCCCCEEECHHHHHHHCCCCCCCCCCEEECCCCCCHHEEEEEEEECCCCC
PEKAVGRKKFEELTPIYPKERLRLETENGRDLSSRLMDIICPIGKGQRGMIVAPPKAGKT
HHHHHHHHHHHHCCCCCCCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHH
TLLKRIAQNISKNNPEVKLIVLLIDERPEEVTDMKRSIDGEVIYSTFDEEPQNHAKVSSI
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
VLERAKRMVEQGRDVVILMDSLTRLSRAYNLTVTPSGRTLSGGLDPGALIMPKKFFGAAR
HHHHHHHHHHCCCCEEEEHHHHHHHHHHEEEEECCCCCEECCCCCCCCEECCHHHHHHHC
NLEEGGSLTILATSLVDTGSRMDDMIFEEFKGTGNMEVHLDRKLQERRIFPAIDIYKSGT
CCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHHHCCCC
RKEDLLFNEEEREASYKIRRVLQKENNIEDVAEKLINLLSKTKNNKEFLQVVLKSNLEN
CCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIEQYENMTLVQLKEQAKELGIKNISKKKKSELIEELKAEFNKQNSNTKVIHKDGVILR
CCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
EKISQKEQGGNKESFSGERVVRNSNNYNNSNDEGKKEQLKDMISSSDSAKGILEILDNNN
HHHHHHHCCCCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCC
FGFLRCRNYLTSEDDIYVSPSQIRRFGLRTGDEVQGKVRIPKDGEKFKALLYVERVNGES
EEEEEECCCCCCCCCEEECHHHHHHHCCCCCCCCCCEEECCCCCCHHEEEEEEEECCCCC
PEKAVGRKKFEELTPIYPKERLRLETENGRDLSSRLMDIICPIGKGQRGMIVAPPKAGKT
HHHHHHHHHHHHCCCCCCCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHH
TLLKRIAQNISKNNPEVKLIVLLIDERPEEVTDMKRSIDGEVIYSTFDEEPQNHAKVSSI
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
VLERAKRMVEQGRDVVILMDSLTRLSRAYNLTVTPSGRTLSGGLDPGALIMPKKFFGAAR
HHHHHHHHHHCCCCEEEEHHHHHHHHHHEEEEECCCCCEECCCCCCCCEECCHHHHHHHC
NLEEGGSLTILATSLVDTGSRMDDMIFEEFKGTGNMEVHLDRKLQERRIFPAIDIYKSGT
CCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHHHCCCC
RKEDLLFNEEEREASYKIRRVLQKENNIEDVAEKLINLLSKTKNNKEFLQVVLKSNLEN
CCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]