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The map label for this gene is hsdM [H]

Identifier: 182682691

GI number: 182682691

Start: 2477905

End: 2479488

Strand: Reverse

Name: hsdM [H]

Synonym: XfasM23_2182

Alternate gene names: 182682691

Gene position: 2479488-2477905 (Counterclockwise)

Preceding gene: 182682693

Following gene: 182682690

Centisome position: 97.78

GC content: 50.76

Gene sequence:

>1584_bases
GTGAGCAATAACAAAGAGCAGGAACGTGCCGAGTTGCACAAAACCATTTGGCGCATCGCCAACGATCTGCGCGGCAGTGT
CGATGGCTGGGACTTTAAGACCTATGTGCTGGGGATGCTGTTTTATCGTTTTATCTCAGAGAATCTGACCAGTTACATCA
ATGCGCAGGAGCCCCGAACCGGTAACGGTAATGTCGATTTTGACTACGCTCAATTGAGTGATGCGGGTGCCGAGTCCGGC
CGCGCGGAAACGGTGAAGGAAAAAGGTTTTTACATCTTGCCTTCTGAGCTGTTTGTCAGGGTGCGGGCCGGAGCGAAGTT
TGACGATAACCTCAATGAGACGTTGAGCAAGGTCTTTGCCAACATTGAGCGCTCGGCGATTGGCAGTGATTCTGAGCAGG
ACATCAAGGGGCTGTTTGATGATCTTGATGTCAACAGCAGTAAGCTCGGCCCGACTGTGCCCAAACGTAACGAAAAGTTG
GTCAAGCTGCTTGAGGCTATCGGCGATTTGCCGTTGACCAGTAGTGAAGGCGGGTTTACGGAAAACACCATCGATTTGTT
CGGTGATGCTTACGAGTATCTGATGCAGATGTACGCTTCCACGGCGGGCAAATCTGGGGGCGAGTTCTACACGCCGCAGG
AAGTCTCTCAATTGCTGACTCAGATCACTGTGGTCGGCAAGACGGAGGTGAATAAGGTGTATGACCCGGCCTGTGGTTCT
GGATCGCTGTTGCTCAACTTCGTCAAGGTGTTAGGACACGATAAGGTGCGTCAGGGCTTCTACGGACAAGAGATTAATCT
CACCACTTACAATCTGTGCCGCATCAATATGTTTCTGCACAATGTGAATTATGAGAAGTTCCACATTGCACACGGTGACA
CGCTCACCGATCCGGCGCATTGGGATGATGAGCCGTTTGAAGCGATCGTTTCCAATCCGCCGTATTCGATCAAATGGGAT
GGTGATTCCAACGCGCTGCTCATCAATGATCCGCGTTTTGCACCGGCGGGGATACTGGCGCCTAAAAGTAAAGCCGATCT
TGCTTTTACGCTGCATATTCTGAGTTGGCTGGCGGTCAATGGCACGGCGGCGATTGTTGAGTTTCCCGGTGTTCTTTATC
GCGGCGGTGCGGAGCAGAAAATCCGCCAATATCTCATCGACAATAATTATGTTGATGCGGTCATCCAGTTGCCGGCTGAT
TTGTTCTTTGGTACGACGATTGCTACTTGCATCATCGTGTTAAAGAAGTCCAAGGGTGATAACGCGACGTTATTCATGGA
TGCTTCTAGTTTGTTTGTACGCAGTGGCACCAAGAACAAGCTGAGCACGGCGCATCAGAAAAAAATCCTTGATGGTTTCA
CGGCACGTCAGGACATTGAGCATTTTGCGCGTTTGGTTGACAACAGTGACATTGCCGCTAACGGCTACAACATCGCTGTG
TCCAGTTACATTGCGCAGGCCGACACGCGTGAGAGTATCGATATCAAGGCGCTCAATCGTGACATTGCCCGCATCGTTGC
AAGACAAGCCGAATTGAGGAAGCAGATTGATGCCATCGTAGCTGATCTAGAAGGGGAGCCATGA

Upstream 100 bases:

>100_bases
CTTCGGCAACGCTTGGTGAGGGTGTTGTGTGTCTCTTGGTATCTTGGCGTTGCAACTGACAATAAACTCTCTCATTCCGC
GGCAGAATGATGTGGCATGA

Downstream 100 bases:

>100_bases
TGAGCGGCCAAGGCCGCGGCGGCATGATCAAGCAGGGGCGTTCTTCTTGGGCGCCATTGATCGTGAGATTGTCCGGCAAC
GGTCAAGACAACGCCTTGCA

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSNNKEQERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTSYINAQEPRTGNGNVDFDYAQLSDAGAESG
RAETVKEKGFYILPSELFVRVRAGAKFDDNLNETLSKVFANIERSAIGSDSEQDIKGLFDDLDVNSSKLGPTVPKRNEKL
VKLLEAIGDLPLTSSEGGFTENTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSQLLTQITVVGKTEVNKVYDPACGS
GSLLLNFVKVLGHDKVRQGFYGQEINLTTYNLCRINMFLHNVNYEKFHIAHGDTLTDPAHWDDEPFEAIVSNPPYSIKWD
GDSNALLINDPRFAPAGILAPKSKADLAFTLHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAVIQLPAD
LFFGTTIATCIIVLKKSKGDNATLFMDASSLFVRSGTKNKLSTAHQKKILDGFTARQDIEHFARLVDNSDIAANGYNIAV
SSYIAQADTRESIDIKALNRDIARIVARQAELRKQIDAIVADLEGEP

Sequences:

>Translated_527_residues
MSNNKEQERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTSYINAQEPRTGNGNVDFDYAQLSDAGAESG
RAETVKEKGFYILPSELFVRVRAGAKFDDNLNETLSKVFANIERSAIGSDSEQDIKGLFDDLDVNSSKLGPTVPKRNEKL
VKLLEAIGDLPLTSSEGGFTENTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSQLLTQITVVGKTEVNKVYDPACGS
GSLLLNFVKVLGHDKVRQGFYGQEINLTTYNLCRINMFLHNVNYEKFHIAHGDTLTDPAHWDDEPFEAIVSNPPYSIKWD
GDSNALLINDPRFAPAGILAPKSKADLAFTLHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAVIQLPAD
LFFGTTIATCIIVLKKSKGDNATLFMDASSLFVRSGTKNKLSTAHQKKILDGFTARQDIEHFARLVDNSDIAANGYNIAV
SSYIAQADTRESIDIKALNRDIARIVARQAELRKQIDAIVADLEGEP
>Mature_526_residues
SNNKEQERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTSYINAQEPRTGNGNVDFDYAQLSDAGAESGR
AETVKEKGFYILPSELFVRVRAGAKFDDNLNETLSKVFANIERSAIGSDSEQDIKGLFDDLDVNSSKLGPTVPKRNEKLV
KLLEAIGDLPLTSSEGGFTENTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSQLLTQITVVGKTEVNKVYDPACGSG
SLLLNFVKVLGHDKVRQGFYGQEINLTTYNLCRINMFLHNVNYEKFHIAHGDTLTDPAHWDDEPFEAIVSNPPYSIKWDG
DSNALLINDPRFAPAGILAPKSKADLAFTLHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAVIQLPADL
FFGTTIATCIIVLKKSKGDNATLFMDASSLFVRSGTKNKLSTAHQKKILDGFTARQDIEHFARLVDNSDIAANGYNIAVS
SYIAQADTRESIDIKALNRDIARIVARQAELRKQIDAIVADLEGEP

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58378; Mature: 58247

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNKEQERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTSYINAQEPRT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GNGNVDFDYAQLSDAGAESGRAETVKEKGFYILPSELFVRVRAGAKFDDNLNETLSKVFA
CCCCEEEEHHHHCCCCCCCCCHHHHHHCCCEEECHHHEEEECCCCCCCCCHHHHHHHHHH
NIERSAIGSDSEQDIKGLFDDLDVNSSKLGPTVPKRNEKLVKLLEAIGDLPLTSSEGGFT
HHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
ENTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSQLLTQITVVGKTEVNKVYDPACGS
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
GSLLLNFVKVLGHDKVRQGFYGQEINLTTYNLCRINMFLHNVNYEKFHIAHGDTLTDPAH
HHHHHHHHHHHCCHHHHCCCCCCEECCEEEEHEEEEEEHCCCCCCEEEEECCCCCCCCCC
WDDEPFEAIVSNPPYSIKWDGDSNALLINDPRFAPAGILAPKSKADLAFTLHILSWLAVN
CCCCHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAVIQLPADLFFGTTIATCIIVLKKSKGD
CCEEEEECCCCEECCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHEECCCCC
NATLFMDASSLFVRSGTKNKLSTAHQKKILDGFTARQDIEHFARLVDNSDIAANGYNIAV
CEEEEEECHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEH
SSYIAQADTRESIDIKALNRDIARIVARQAELRKQIDAIVADLEGEP
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNNKEQERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTSYINAQEPRT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GNGNVDFDYAQLSDAGAESGRAETVKEKGFYILPSELFVRVRAGAKFDDNLNETLSKVFA
CCCCEEEEHHHHCCCCCCCCCHHHHHHCCCEEECHHHEEEECCCCCCCCCHHHHHHHHHH
NIERSAIGSDSEQDIKGLFDDLDVNSSKLGPTVPKRNEKLVKLLEAIGDLPLTSSEGGFT
HHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
ENTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSQLLTQITVVGKTEVNKVYDPACGS
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
GSLLLNFVKVLGHDKVRQGFYGQEINLTTYNLCRINMFLHNVNYEKFHIAHGDTLTDPAH
HHHHHHHHHHHCCHHHHCCCCCCEECCEEEEHEEEEEEHCCCCCCEEEEECCCCCCCCCC
WDDEPFEAIVSNPPYSIKWDGDSNALLINDPRFAPAGILAPKSKADLAFTLHILSWLAVN
CCCCHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAVIQLPADLFFGTTIATCIIVLKKSKGD
CCEEEEECCCCEECCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHEECCCCC
NATLFMDASSLFVRSGTKNKLSTAHQKKILDGFTARQDIEHFARLVDNSDIAANGYNIAV
CEEEEEECHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEH
SSYIAQADTRESIDIKALNRDIARIVARQAELRKQIDAIVADLEGEP
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]