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The map label for this gene is sppA [H]

Identifier: 182682655

GI number: 182682655

Start: 2421434

End: 2423335

Strand: Reverse

Name: sppA [H]

Synonym: XfasM23_2145

Alternate gene names: 182682655

Gene position: 2423335-2421434 (Counterclockwise)

Preceding gene: 182682656

Following gene: 182682654

Centisome position: 95.57

GC content: 50.47

Gene sequence:

>1902_bases
ATGAACCAGTCTGTGCCTAGAAATCCCATTGCCCGTTTCTTTATTGGCTTATGGGGAGCGATGAATTTTACCCGCCGCTT
AATTCTCAATTTGGTGTTTTTCGGTTTCTTGTTTTTCTTGTTATTGATGATGTTAATTGGAATGACGCACGGCTTGAAAG
GCAAGCAGCTCGACGATCGCACGACTCTATTGATTGCGCCGCAAGGCACATTGGTGGAACAGTTCAGTGCCGATCCGGCC
AGTCGTGCATTAGCCAAAGCGACTGGTGATAAGAGTGGGCAGGAAGTTCAACTGCGTGATCTGATCCGTGTGATTGAGGC
TGCGGCTAAGGATAAGAAGATTGAACGTGTTCTGCTGGATTTGGACAAGTTGCAGCCATCAGGGTATGCCTCATTGCGTG
AGGTCGTTGCTGCGTTACAGAACCTCAAAGATTCAGGGAAACAGTTAGTTGCTTTCAGTGAAAGTATGACTCAGTCGCAG
TATCTGCTTGCCGCACAGGCGGATGAGATTTATTTGGATCCAATGGGCAGTGTTTTGCTTGAGGGTTTGGCTCACTACCG
TCAGTATTTCCGCAAGGGATTACAAGAAAAATTGGGTGTGGATGTTCATCTTTTCCGTGTCGGCGAATACAAATCCGCGG
CTGAGCCTTTTGTTCTTGATGCGGCCTCAGCCGATGCCAAGGAGGCCGATTTGTTCTGGATGAACGATATATGGCAACGC
TATTTGGCCGATATCGCTAAGGCACGTAAGCTCGATTCGGCGCACCTCAATACTATAGTCGAAAGACTGCCGCAGGATAT
TGCTGCTAATCATGGTGATTTGGCCAAGTATGCATTGGCGCAGAAACTGGTAGATGGCTTGAGAACTCGCGAGCAAGTGA
ACCAGTTAATGATCCAGCGTGGTGTTGCCGATAAAAAGGCCGATGGTGGTTTCCGTAATGTGGACTTCGGTACTTATTTA
AGTTTGCTGGATGCACAGAGTGCGCTACTAGGCACGTCCTCGCAGATTGCCGTCGTTGTGGCAGCGGGTGAAATAAAAGG
AGGTGATCAGCCTGCCGGTTATATTGGTGGTGAGTCGACCAGTATGCTTTTGCGTAAGGTGCGTGATGAAGACGCGGTCA
AAGCGGTGGTGCTTCGTGTAAATTCTCCCGGTGGAGAAGTCTTCGCTTCTGAGCAGATTCGCCGTGAAGTCGTTGCACTG
AGGAAGGTTGGCAAGCCGGTCGTGGTGTCGATGGGTGATATGGCTGCTTCCGGAGGGTATTGGATTAGCATGGATGCTGA
TCGCATTTATGCTGATCCATCGACGATCAGTGGTTCGATTGGTATCTTCGGCTTGGTACCCAATATCACTCGTACGCTTG
ATAAAGTGGGAGTGCACACGGATGGCGTGGGTACGACCCGTTTTGCAGGTGCACTTGATATCACTCGTGCGCTGGATCCG
CAGGTGGGACAGATATTCCAGAATGTGATCGACAAAGGCTACGCAGATTTCACAACTAAGGTTGCGCAGGCGCGTCACCG
CTCCGTGAAAGAGATTGACAGTATTGCTCGCGGTCGTGTATGGAGCGGCGCGCAGGCGAAAGAACGTGGTTTGGTCGATG
AACTTGGGGGAGTGCGCGAGGCGATTACTGATGCTGCAAAGCGTGCCAAGCTTGGCGAGGGCCACTACAGTGTGCGTTAT
GTTGATAAGGCAGTCAGCCCATTTATGCAGTTCATCATCAACATGATGTCCACTCGGATTGGGAACTGGGCATTGGGAGA
TTCAGGCTTGGCTCGGGTGCTGCTCACCCGTATTTCTCCGGAAACAGAGGTGCAGTTGCGTTTTGTTGAAGATGCAGTGC
GTGAGGCCAAAACGGGCATGCTTCCAGTCAAACCCTTGGTTTACTGTTTCTGTGGACTTTAG

Upstream 100 bases:

>100_bases
TTTGCCGGTATTCCCTAATTGTCAGTCACAGCGCGGTTATGCTGACACCCGCTGCGCTGATTCGCGTTCAAAATGATGTC
AGTTTTTCAAGGAGCGTTTC

Downstream 100 bases:

>100_bases
CTAGTTTGTGGCGCCCTATGGTGCGATTGTTTGATTCTGTTTGCTGTTTTTCGACCGGAATCTTCTTTTGTTTTTTAAGG
AGTGGCGTGTAAGAGGCTGA

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MNQSVPRNPIARFFIGLWGAMNFTRRLILNLVFFGFLFFLLLMMLIGMTHGLKGKQLDDRTTLLIAPQGTLVEQFSADPA
SRALAKATGDKSGQEVQLRDLIRVIEAAAKDKKIERVLLDLDKLQPSGYASLREVVAALQNLKDSGKQLVAFSESMTQSQ
YLLAAQADEIYLDPMGSVLLEGLAHYRQYFRKGLQEKLGVDVHLFRVGEYKSAAEPFVLDAASADAKEADLFWMNDIWQR
YLADIAKARKLDSAHLNTIVERLPQDIAANHGDLAKYALAQKLVDGLRTREQVNQLMIQRGVADKKADGGFRNVDFGTYL
SLLDAQSALLGTSSQIAVVVAAGEIKGGDQPAGYIGGESTSMLLRKVRDEDAVKAVVLRVNSPGGEVFASEQIRREVVAL
RKVGKPVVVSMGDMAASGGYWISMDADRIYADPSTISGSIGIFGLVPNITRTLDKVGVHTDGVGTTRFAGALDITRALDP
QVGQIFQNVIDKGYADFTTKVAQARHRSVKEIDSIARGRVWSGAQAKERGLVDELGGVREAITDAAKRAKLGEGHYSVRY
VDKAVSPFMQFIINMMSTRIGNWALGDSGLARVLLTRISPETEVQLRFVEDAVREAKTGMLPVKPLVYCFCGL

Sequences:

>Translated_633_residues
MNQSVPRNPIARFFIGLWGAMNFTRRLILNLVFFGFLFFLLLMMLIGMTHGLKGKQLDDRTTLLIAPQGTLVEQFSADPA
SRALAKATGDKSGQEVQLRDLIRVIEAAAKDKKIERVLLDLDKLQPSGYASLREVVAALQNLKDSGKQLVAFSESMTQSQ
YLLAAQADEIYLDPMGSVLLEGLAHYRQYFRKGLQEKLGVDVHLFRVGEYKSAAEPFVLDAASADAKEADLFWMNDIWQR
YLADIAKARKLDSAHLNTIVERLPQDIAANHGDLAKYALAQKLVDGLRTREQVNQLMIQRGVADKKADGGFRNVDFGTYL
SLLDAQSALLGTSSQIAVVVAAGEIKGGDQPAGYIGGESTSMLLRKVRDEDAVKAVVLRVNSPGGEVFASEQIRREVVAL
RKVGKPVVVSMGDMAASGGYWISMDADRIYADPSTISGSIGIFGLVPNITRTLDKVGVHTDGVGTTRFAGALDITRALDP
QVGQIFQNVIDKGYADFTTKVAQARHRSVKEIDSIARGRVWSGAQAKERGLVDELGGVREAITDAAKRAKLGEGHYSVRY
VDKAVSPFMQFIINMMSTRIGNWALGDSGLARVLLTRISPETEVQLRFVEDAVREAKTGMLPVKPLVYCFCGL
>Mature_633_residues
MNQSVPRNPIARFFIGLWGAMNFTRRLILNLVFFGFLFFLLLMMLIGMTHGLKGKQLDDRTTLLIAPQGTLVEQFSADPA
SRALAKATGDKSGQEVQLRDLIRVIEAAAKDKKIERVLLDLDKLQPSGYASLREVVAALQNLKDSGKQLVAFSESMTQSQ
YLLAAQADEIYLDPMGSVLLEGLAHYRQYFRKGLQEKLGVDVHLFRVGEYKSAAEPFVLDAASADAKEADLFWMNDIWQR
YLADIAKARKLDSAHLNTIVERLPQDIAANHGDLAKYALAQKLVDGLRTREQVNQLMIQRGVADKKADGGFRNVDFGTYL
SLLDAQSALLGTSSQIAVVVAAGEIKGGDQPAGYIGGESTSMLLRKVRDEDAVKAVVLRVNSPGGEVFASEQIRREVVAL
RKVGKPVVVSMGDMAASGGYWISMDADRIYADPSTISGSIGIFGLVPNITRTLDKVGVHTDGVGTTRFAGALDITRALDP
QVGQIFQNVIDKGYADFTTKVAQARHRSVKEIDSIARGRVWSGAQAKERGLVDELGGVREAITDAAKRAKLGEGHYSVRY
VDKAVSPFMQFIINMMSTRIGNWALGDSGLARVLLTRISPETEVQLRFVEDAVREAKTGMLPVKPLVYCFCGL

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=576, Percent_Identity=40.4513888888889, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1787527, Length=196, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 69274; Mature: 69274

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSVPRNPIARFFIGLWGAMNFTRRLILNLVFFGFLFFLLLMMLIGMTHGLKGKQLDDR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTLLIAPQGTLVEQFSADPASRALAKATGDKSGQEVQLRDLIRVIEAAAKDKKIERVLLD
EEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LDKLQPSGYASLREVVAALQNLKDSGKQLVAFSESMTQSQYLLAAQADEIYLDPMGSVLL
HHHCCCCCHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHEECCCCEEECCHHHHHH
EGLAHYRQYFRKGLQEKLGVDVHLFRVGEYKSAAEPFVLDAASADAKEADLFWMNDIWQR
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHH
YLADIAKARKLDSAHLNTIVERLPQDIAANHGDLAKYALAQKLVDGLRTREQVNQLMIQR
HHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVADKKADGGFRNVDFGTYLSLLDAQSALLGTSSQIAVVVAAGEIKGGDQPAGYIGGEST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHH
SMLLRKVRDEDAVKAVVLRVNSPGGEVFASEQIRREVVALRKVGKPVVVSMGDMAASGGY
HHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
WISMDADRIYADPSTISGSIGIFGLVPNITRTLDKVGVHTDGVGTTRFAGALDITRALDP
EEEECCCEEECCCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCH
QVGQIFQNVIDKGYADFTTKVAQARHRSVKEIDSIARGRVWSGAQAKERGLVDELGGVRE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHH
AITDAAKRAKLGEGHYSVRYVDKAVSPFMQFIINMMSTRIGNWALGDSGLARVLLTRISP
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
ETEVQLRFVEDAVREAKTGMLPVKPLVYCFCGL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNQSVPRNPIARFFIGLWGAMNFTRRLILNLVFFGFLFFLLLMMLIGMTHGLKGKQLDDR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTLLIAPQGTLVEQFSADPASRALAKATGDKSGQEVQLRDLIRVIEAAAKDKKIERVLLD
EEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LDKLQPSGYASLREVVAALQNLKDSGKQLVAFSESMTQSQYLLAAQADEIYLDPMGSVLL
HHHCCCCCHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHEECCCCEEECCHHHHHH
EGLAHYRQYFRKGLQEKLGVDVHLFRVGEYKSAAEPFVLDAASADAKEADLFWMNDIWQR
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHH
YLADIAKARKLDSAHLNTIVERLPQDIAANHGDLAKYALAQKLVDGLRTREQVNQLMIQR
HHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVADKKADGGFRNVDFGTYLSLLDAQSALLGTSSQIAVVVAAGEIKGGDQPAGYIGGEST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHH
SMLLRKVRDEDAVKAVVLRVNSPGGEVFASEQIRREVVALRKVGKPVVVSMGDMAASGGY
HHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
WISMDADRIYADPSTISGSIGIFGLVPNITRTLDKVGVHTDGVGTTRFAGALDITRALDP
EEEECCCEEECCCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCH
QVGQIFQNVIDKGYADFTTKVAQARHRSVKEIDSIARGRVWSGAQAKERGLVDELGGVRE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHH
AITDAAKRAKLGEGHYSVRYVDKAVSPFMQFIINMMSTRIGNWALGDSGLARVLLTRISP
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
ETEVQLRFVEDAVREAKTGMLPVKPLVYCFCGL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]