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The map label for this gene is xpsO [H]

Identifier: 182682541

GI number: 182682541

Start: 2270084

End: 2270947

Strand: Reverse

Name: xpsO [H]

Synonym: XfasM23_2029

Alternate gene names: 182682541

Gene position: 2270947-2270084 (Counterclockwise)

Preceding gene: 182682542

Following gene: 182682540

Centisome position: 89.56

GC content: 51.04

Gene sequence:

>864_bases
ATGGCATTTCTTGATCAGCACCCCGGACTCGGCTATCCCCTTGCGTCCGGTTTGGGGTTGTTGCTTGGTAGTTTTCTCAA
TGTGGTGATCCTGCGTTTGCCGCGCCGTTTGGAATGGCAATGGAAGCGCGATGCGCGCGAAATCCTGGAAATGCCGGACA
TTTATGATCCGCCACCGCCGGGAATTGTGGTCGAGCCTTCCCACGATCCAGTGACTGGTGATCGGTTGAAGTGGTGGGAA
AACATTCCGGTGCTTAGCTGGTTGTTGCTACGTGGACGTTCGCGTTACAGCGGCAAGTCAATCTCGTTGCAGTATCCGTT
GGTCGAATTGCTGACTTGCCTGCTGGTGCTGGCCAGTGTTTGGCGTTTTGGATTCGGTTGGCATGGTTTTGGGGCAATTG
TGTTCAGTTGTTTCCTGGTTGCGTTGGCTGGGATTGATTTGCGTACTCGGCTCTTACCTGACCAGTTAACTTTAATGCTC
ATGTGGCTGGGTTTGATTGGATCGGCGGATAACTTGTTTATGGCAGCCAAACCTGCGTTATTGGGGGCTGCAGTTGGTTA
TTTATCTCTGTGGATTGTGGCGGCGTTGTTCAAACTGATCTTTAACAAGGAAGGGATGGGCCATGGTGACTTCAAAATGC
TGGGCGCGCTTGGGGCATGGTGTGGGTTAAAGGGTATTCTCCCGATCATTTTGATTTCATCACTGGCTGGAGTGGTATTG
GGCTCTGTTTGGCTTGTTGTGCGTGGTCATAACCGTACGACGATGATTCCGTTTGGACCTTATCTGGCAGTTGGTGGATG
GATCGTGTTCTTTTTCGGCGATCAGATCATCCATGCTTATCTGGAGCTATTAAGTATCGGGTGA

Upstream 100 bases:

>100_bases
ACCGCTGATCATGGTCTTTATTGGTGTTATCGTCGGCGGCATGGTCATTGCTATGTATTTGCCGATCTTTAAGCTTGCTT
CTGTCGTTGGATAAAACGTT

Downstream 100 bases:

>100_bases
GGTTGGAGTTGATGAGTGTCTATGCGGTTGGTCTCACTGGAGGGGTCGCTTGCGGTAAGAGCTTGCTGGCGCAGCTGTTC
GAGGCGTTAAACACGACGGT

Product: peptidase A24A domain-containing protein

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWE
NIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLML
MWLGLIGSADNLFMAAKPALLGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVL
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG

Sequences:

>Translated_287_residues
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWE
NIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLML
MWLGLIGSADNLFMAAKPALLGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVL
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG
>Mature_286_residues
AFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWEN
IPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLMLM
WLGLIGSADNLFMAAKPALLGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVLG
SVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=265, Percent_Identity=33.2075471698113, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789732, Length=135, Percent_Identity=45.1851851851852, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31705; Mature: 31574

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCCCCCC
GIVVEPSHDPVTGDRLKWWENIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASV
CEEECCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
WRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLMLMWLGLIGSADNLFMAAKPAL
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG
HHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCCCCCC
GIVVEPSHDPVTGDRLKWWENIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASV
CEEECCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
WRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLMLMWLGLIGSADNLFMAAKPAL
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG
HHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8817497; 12024217 [H]