Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 182682396

GI number: 182682396

Start: 2085850

End: 2087343

Strand: Direct

Name: miaB

Synonym: XfasM23_1882

Alternate gene names: 182682396

Gene position: 2085850-2087343 (Clockwise)

Preceding gene: 182682387

Following gene: 182682397

Centisome position: 82.26

GC content: 54.95

Gene sequence:

>1494_bases
ATGACCGGGACATCAAACATCCCCACACACGGCAAAGAGCACAAAGACGCCCCCGCACTGCTGCCATTGCCCGCACCAAA
CACACACCATACCCACGCAGCACATCCTGGCGACCCATCACACGACCGCCACCCATCGCGCGGAAAGCTCTTTATTAAAA
CCCACGGCTGCCAAATGAACGAATACGACTCGGCCAAAATGGCCGACGTACTCACCACCACCGAAGCACTGGAACTGACC
GACAATCCCGAAGAAGCAGACATTATTCTCATCAACACCTGTTCAATCCGCGAAAAGGCCCAAGAAAAAGTATTCAGCCA
ACTCGGACGCTGGAGAGCACTGAAAACCAATGGCAGAGATGTCATCATCGGCGTCGGCGGTTGCGTTGCCTCCCAAGAAG
GAGAAACAATCGTCAAACGCGCCCCTTACGTTGACCTTGTCTTTGGCCCGCAAACATTGCATCGCCTCCCAGACATGATC
CGCGCCCGACGTGAACAGAACCGACCACAGGTCGACATCAGCTTCCCGGAAATCGAAAAATTCGACCACCTTCCCACACC
GCGTGCCGAAGGCCCAAGCGCATTCGTCTCGATTATGGAAGGCTGCTCCAAATATTGCTCATTTTGTGTCGTTCCCTACA
CCCGCGGCGAAGAAGTCAGCCGCCCATTTGAAGACGTGTTGACCGAAATAGCACACCTAGCGACCCAAGGCGTCCGTGAA
ATCAACCTGCTCGGCCAAAACGTCAACGCCTATCGCGGAGCGATGGACCCTGGTCCATCCAACAACACCAACCCTGCGCC
CCCCCCTTATGCCGATCTCGGCCTCCTCATCCGTGCAATCGCCCAATTCGAAAGCATCGGACGTATCCGCTTCACCACAT
CCCATCCCCTGGAATTTTCGGATTCCCTCGTAGAAGCCTACCGCGACGTCCCACAACTGGCTAACCACCTGCACCTTCCC
GTTCAATCCGGCAGCGACCGTATCCTCTCAGCGATGAAACGTGGCTACACCGCACTGGAATTCAAATCCAAAATCCGCAA
ACTGCGCGCAGTTCGCCCGGACATTTCGATCAGCTCCGACTTCATCATCGGCTTCCCTGGTGAAAGCGACACCGACTTCC
AGAAGACCATGCAGCTCATCAAAGACATTGGTTTCGACCAGAGCTTCTCTTTCATCTACTCCCGCCGCCCTGGCACACCG
GCCTCCAACCTTGAGGACCACACACCCGATGAAATCAAACGCACCCGCCTAGAACACCTACAAAAGCACATCAACGCCTA
TGCCGCCGACATCTCAAAACGCATGATTGGCACCGTACAAACCGTGCTGGTGGAAGGACCATCAAAAAAGAACCCTAACG
AACTGACCGGAAAAACCGAAAACATGCGGCCAGTCAACTTCCCTGGCCACCCACGCCTCATCGGACAATTTATTGACGTA
CACATCACCGAAGCACTGACCAACTCATTACGCGGCCGCGTGCATACCAACTGA

Upstream 100 bases:

>100_bases
GACAACAGCACAGCCAACGCCCCTACATCTCCATCCCCCTGAAGCTCCTGCAACAACACGCTAAACTACACCCCTTACCC
ACCCGACTGGATTAAGCACC

Downstream 100 bases:

>100_bases
CAACGAAAACATGACCTCCCACATCCCTTCTGACGTTTTTTTCATCAAAAACAAAAAATACTTACACACCAAAAAATTAC
AGTGATGACCCACCGCTTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTGTSNIPTHGKEHKDAPALLPLPAPNTHHTHAAHPGDPSHDRHPSRGKLFIKTHGCQMNEYDSAKMADVLTTTEALELT
DNPEEADIILINTCSIREKAQEKVFSQLGRWRALKTNGRDVIIGVGGCVASQEGETIVKRAPYVDLVFGPQTLHRLPDMI
RARREQNRPQVDISFPEIEKFDHLPTPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLTEIAHLATQGVRE
INLLGQNVNAYRGAMDPGPSNNTNPAPPPYADLGLLIRAIAQFESIGRIRFTTSHPLEFSDSLVEAYRDVPQLANHLHLP
VQSGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIIGFPGESDTDFQKTMQLIKDIGFDQSFSFIYSRRPGTP
ASNLEDHTPDEIKRTRLEHLQKHINAYAADISKRMIGTVQTVLVEGPSKKNPNELTGKTENMRPVNFPGHPRLIGQFIDV
HITEALTNSLRGRVHTN

Sequences:

>Translated_497_residues
MTGTSNIPTHGKEHKDAPALLPLPAPNTHHTHAAHPGDPSHDRHPSRGKLFIKTHGCQMNEYDSAKMADVLTTTEALELT
DNPEEADIILINTCSIREKAQEKVFSQLGRWRALKTNGRDVIIGVGGCVASQEGETIVKRAPYVDLVFGPQTLHRLPDMI
RARREQNRPQVDISFPEIEKFDHLPTPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLTEIAHLATQGVRE
INLLGQNVNAYRGAMDPGPSNNTNPAPPPYADLGLLIRAIAQFESIGRIRFTTSHPLEFSDSLVEAYRDVPQLANHLHLP
VQSGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIIGFPGESDTDFQKTMQLIKDIGFDQSFSFIYSRRPGTP
ASNLEDHTPDEIKRTRLEHLQKHINAYAADISKRMIGTVQTVLVEGPSKKNPNELTGKTENMRPVNFPGHPRLIGQFIDV
HITEALTNSLRGRVHTN
>Mature_496_residues
TGTSNIPTHGKEHKDAPALLPLPAPNTHHTHAAHPGDPSHDRHPSRGKLFIKTHGCQMNEYDSAKMADVLTTTEALELTD
NPEEADIILINTCSIREKAQEKVFSQLGRWRALKTNGRDVIIGVGGCVASQEGETIVKRAPYVDLVFGPQTLHRLPDMIR
ARREQNRPQVDISFPEIEKFDHLPTPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLTEIAHLATQGVREI
NLLGQNVNAYRGAMDPGPSNNTNPAPPPYADLGLLIRAIAQFESIGRIRFTTSHPLEFSDSLVEAYRDVPQLANHLHLPV
QSGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIIGFPGESDTDFQKTMQLIKDIGFDQSFSFIYSRRPGTPA
SNLEDHTPDEIKRTRLEHLQKHINAYAADISKRMIGTVQTVLVEGPSKKNPNELTGKTENMRPVNFPGHPRLIGQFIDVH
ITEALTNSLRGRVHTN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=36.1169102296451, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=36.1169102296451, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI93277076, Length=473, Percent_Identity=26.8498942917548, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=64.5739910313901, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1787057, Length=383, Percent_Identity=27.9373368146214, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=34.9240780911063, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=333, Percent_Identity=26.7267267267267, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356207, Length=503, Percent_Identity=32.4055666003976, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=27.9661016949153, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_XYLF2 (B2I8U1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830556.1
- GeneID:   6203163
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1882
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 55315; Mature: 55184

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTSNIPTHGKEHKDAPALLPLPAPNTHHTHAAHPGDPSHDRHPSRGKLFIKTHGCQMN
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
EYDSAKMADVLTTTEALELTDNPEEADIILINTCSIREKAQEKVFSQLGRWRALKTNGRD
CCCCHHHHHHHHHHHHEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCE
VIIGVGGCVASQEGETIVKRAPYVDLVFGPQTLHRLPDMIRARREQNRPQVDISFPEIEK
EEEECCCEECCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
FDHLPTPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLTEIAHLATQGVRE
HCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHH
INLLGQNVNAYRGAMDPGPSNNTNPAPPPYADLGLLIRAIAQFESIGRIRFTTSHPLEFS
HHHHCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHH
DSLVEAYRDVPQLANHLHLPVQSGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSD
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
FIIGFPGESDTDFQKTMQLIKDIGFDQSFSFIYSRRPGTPASNLEDHTPDEIKRTRLEHL
EEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHH
QKHINAYAADISKRMIGTVQTVLVEGPSKKNPNELTGKTENMRPVNFPGHPRLIGQFIDV
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHH
HITEALTNSLRGRVHTN
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TGTSNIPTHGKEHKDAPALLPLPAPNTHHTHAAHPGDPSHDRHPSRGKLFIKTHGCQMN
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
EYDSAKMADVLTTTEALELTDNPEEADIILINTCSIREKAQEKVFSQLGRWRALKTNGRD
CCCCHHHHHHHHHHHHEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCE
VIIGVGGCVASQEGETIVKRAPYVDLVFGPQTLHRLPDMIRARREQNRPQVDISFPEIEK
EEEECCCEECCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
FDHLPTPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLTEIAHLATQGVRE
HCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHH
INLLGQNVNAYRGAMDPGPSNNTNPAPPPYADLGLLIRAIAQFESIGRIRFTTSHPLEFS
HHHHCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHH
DSLVEAYRDVPQLANHLHLPVQSGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSD
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
FIIGFPGESDTDFQKTMQLIKDIGFDQSFSFIYSRRPGTPASNLEDHTPDEIKRTRLEHL
EEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHH
QKHINAYAADISKRMIGTVQTVLVEGPSKKNPNELTGKTENMRPVNFPGHPRLIGQFIDV
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHH
HITEALTNSLRGRVHTN
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA