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The map label for this gene is fmt

Identifier: 182682378

GI number: 182682378

Start: 2068554

End: 2069477

Strand: Reverse

Name: fmt

Synonym: XfasM23_1864

Alternate gene names: 182682378

Gene position: 2069477-2068554 (Counterclockwise)

Preceding gene: 182682379

Following gene: 182682377

Centisome position: 81.61

GC content: 58.98

Gene sequence:

>924_bases
ATGAGAATTGTTTTTGCCGGTACACCGGATTTTGCGGTGCCATCCTTGCGCTCGGTGACTCAGCGAGCCGATGTTGTTGC
GGTCTACACGCAACCAGATCGCCCCGCTGGTCGTGGCCGTGAACTGATGCCTTCGCCAGTCAAGCTGGAGGCGGTCGCGC
GTGGTTTGCCGGTGTACCAACCCCAGACCTTGCGTTCGCCCGAGGTGTTGGAGCAGCTACGTGCATTGCGTCCAGATCTG
ATTGTTGTCGTTGCTTACGGGGTGATTCTCCCCGAGGCGGTGTTGACGATTCCTGATGATGGTTGCTGGAACGTGCATGC
TTCCCTGTTACCGCGCTGGCGCGGCGCTGCACCGATTCAGCGTGCGATTGAGGCCGGTGATACCGAAACCGGCGTTTGTC
TGATGCAGATGGAAGCGGGTCTTGATACTGGCCCTGTGTTGATGTCCCTGAAGACTCCGATTAATGCGCATGAAACCAGT
GGGCAGTTACATGATCGTCTTGCTGAGATGGGAGCACAGCTACTTTCTGATGGCCTGGGCTTGTTGCGTGCTGGGCTACG
GCCTGTGCCACAGCCGCAGTTGGCTGTTGGTGTGACCTATGCGCACAAACTCGGCAAGGTTGAATCCCGGCTTGATTGGG
AGCAACCGGCCGAACGGCTTGCCTGCAGGGTACGGGCGTTCCAGCCATGGCCGGTGGCTGAGGTCGTATTGAGTGGTGAG
CGGGTGCGTATCCATGAGGCGTTGGCTCTGGATCTGGATCACAGCCAGCCGCCGGGTACGGTGTTGGCGGCGAGTAAAGA
GGGGATTGATGTGGCCTGTGTGCAGGGCGCGTTGCGTTTGTGCAGATTACAGAGGGAAGGTGGGAAAGCGATCACCGTGG
CTGATTACCTTAACGCCCGCCGAGACTTGCAGGTCCGTGCGTGA

Upstream 100 bases:

>100_bases
GAGGTAGGATGAGGTTTGTATTGGAGAGTGGTGCTGGTCTCGCTTGGGAGTCCACCTCATCCACAGGCCCTAATATTCCC
CATCAGGATCACCATTATTT

Downstream 100 bases:

>100_bases
GCGATGGCATTGCACCGCGTGTGGTTGCGGCGCGGGTGCTAGCTTTGGTCGTGGATCAGGGGCGTTCGCTCAAGACGGAG
CTGGCCGCAGCGTTGCCAAC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRIVFAGTPDFAVPSLRSVTQRADVVAVYTQPDRPAGRGRELMPSPVKLEAVARGLPVYQPQTLRSPEVLEQLRALRPDL
IVVVAYGVILPEAVLTIPDDGCWNVHASLLPRWRGAAPIQRAIEAGDTETGVCLMQMEAGLDTGPVLMSLKTPINAHETS
GQLHDRLAEMGAQLLSDGLGLLRAGLRPVPQPQLAVGVTYAHKLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGE
RVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNARRDLQVRA

Sequences:

>Translated_307_residues
MRIVFAGTPDFAVPSLRSVTQRADVVAVYTQPDRPAGRGRELMPSPVKLEAVARGLPVYQPQTLRSPEVLEQLRALRPDL
IVVVAYGVILPEAVLTIPDDGCWNVHASLLPRWRGAAPIQRAIEAGDTETGVCLMQMEAGLDTGPVLMSLKTPINAHETS
GQLHDRLAEMGAQLLSDGLGLLRAGLRPVPQPQLAVGVTYAHKLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGE
RVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNARRDLQVRA
>Mature_307_residues
MRIVFAGTPDFAVPSLRSVTQRADVVAVYTQPDRPAGRGRELMPSPVKLEAVARGLPVYQPQTLRSPEVLEQLRALRPDL
IVVVAYGVILPEAVLTIPDDGCWNVHASLLPRWRGAAPIQRAIEAGDTETGVCLMQMEAGLDTGPVLMSLKTPINAHETS
GQLHDRLAEMGAQLLSDGLGLLRAGLRPVPQPQLAVGVTYAHKLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGE
RVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNARRDLQVRA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=291, Percent_Identity=28.8659793814433, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI164663775, Length=318, Percent_Identity=30.188679245283, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI21614513, Length=295, Percent_Identity=26.7796610169492, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=51.6339869281046, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788589, Length=271, Percent_Identity=29.1512915129151, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=261, Percent_Identity=27.9693486590038, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319458, Length=349, Percent_Identity=25.214899713467, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550868, Length=307, Percent_Identity=31.9218241042345, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=34.2105263157895, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585660, Length=262, Percent_Identity=28.6259541984733, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_XYLF2 (B2I8S3)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830538.1
- ProteinModelPortal:   B2I8S3
- SMR:   B2I8S3
- GeneID:   6202180
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1864
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33310; Mature: 33310

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVFAGTPDFAVPSLRSVTQRADVVAVYTQPDRPAGRGRELMPSPVKLEAVARGLPVYQ
CEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
PQTLRSPEVLEQLRALRPDLIVVVAYGVILPEAVLTIPDDGCWNVHASLLPRWRGAAPIQ
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCHHEEECCCCCCCCCHHHHCCCCCCCCHHH
RAIEAGDTETGVCLMQMEAGLDTGPVLMSLKTPINAHETSGQLHDRLAEMGAQLLSDGLG
HHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLRAGLRPVPQPQLAVGVTYAHKLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGE
HHHHCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHCCC
RVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNAR
EEEEEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC
RDLQVRA
CCEEECC
>Mature Secondary Structure
MRIVFAGTPDFAVPSLRSVTQRADVVAVYTQPDRPAGRGRELMPSPVKLEAVARGLPVYQ
CEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
PQTLRSPEVLEQLRALRPDLIVVVAYGVILPEAVLTIPDDGCWNVHASLLPRWRGAAPIQ
CCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCHHEEECCCCCCCCCHHHHCCCCCCCCHHH
RAIEAGDTETGVCLMQMEAGLDTGPVLMSLKTPINAHETSGQLHDRLAEMGAQLLSDGLG
HHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLRAGLRPVPQPQLAVGVTYAHKLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGE
HHHHCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHCCC
RVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNAR
EEEEEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC
RDLQVRA
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA