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The map label for this gene is def

Identifier: 182682379

GI number: 182682379

Start: 2069583

End: 2070095

Strand: Reverse

Name: def

Synonym: XfasM23_1865

Alternate gene names: 182682379

Gene position: 2070095-2069583 (Counterclockwise)

Preceding gene: 182682385

Following gene: 182682378

Centisome position: 81.64

GC content: 53.8

Gene sequence:

>513_bases
ATGGCCTTATTGCCGATTCTTGAGTTTCCTGATCCTCGCCTGCGGACCAAGGCGGTGCGTGTGGGGGTTGCCGAGGTGGT
TTCATCGTCGTTCCAGACTCTGCTTGATGACATGTTCGAGACGATGTATGCCGCCCCTGGTATTGGCTTGGCTGCGACTC
AAGTGAATGTGCATCAACGCTTTATGGTGATTGATGTCAGCGAGGAAAAAAATGTGCCGATGGTCTTTATTAATCCGGAG
ATCGTCACCAGGGAGGGCGATCAGGTGTTTCAGGAAGGCTGTTTATCGGTGCCTGGAATCCATGCCGATGTGACCCGTGC
TCTTAGCATTGTGGTGCGCTTTCTGGACCGACACGGCGACGAACAACAGCTCACCGCTGAGGGGCTGTTGGCGGTATGTA
TCCAGCATGAGATGGACCATCTGGATGGCAAGCTTTTCATTGATTATCTCTCTCCTCTCAAGCGTGACATGGTGCGTAGG
AAGCTTGAGAAGCAGCGTCGGCGCGCCTCCTGA

Upstream 100 bases:

>100_bases
ATAGTCCAGATTCTAAAGAGCTGCGCAAGTATTGAGCTAGAATCGCGTTGAAAGTTTTCCGGATATTCCGGCCTCTACGA
ATATGAATTGAGGTGCCGCT

Downstream 100 bases:

>100_bases
CGCCCGAGGTAGGATGAGGTTTGTATTGGAGAGTGGTGCTGGTCTCGCTTGGGAGTCCACCTCATCCACAGGCCCTAATA
TTCCCCATCAGGATCACCAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MALLPILEFPDPRLRTKAVRVGVAEVVSSSFQTLLDDMFETMYAAPGIGLAATQVNVHQRFMVIDVSEEKNVPMVFINPE
IVTREGDQVFQEGCLSVPGIHADVTRALSIVVRFLDRHGDEQQLTAEGLLAVCIQHEMDHLDGKLFIDYLSPLKRDMVRR
KLEKQRRRAS

Sequences:

>Translated_170_residues
MALLPILEFPDPRLRTKAVRVGVAEVVSSSFQTLLDDMFETMYAAPGIGLAATQVNVHQRFMVIDVSEEKNVPMVFINPE
IVTREGDQVFQEGCLSVPGIHADVTRALSIVVRFLDRHGDEQQLTAEGLLAVCIQHEMDHLDGKLFIDYLSPLKRDMVRR
KLEKQRRRAS
>Mature_169_residues
ALLPILEFPDPRLRTKAVRVGVAEVVSSSFQTLLDDMFETMYAAPGIGLAATQVNVHQRFMVIDVSEEKNVPMVFINPEI
VTREGDQVFQEGCLSVPGIHADVTRALSIVVRFLDRHGDEQQLTAEGLLAVCIQHEMDHLDGKLFIDYLSPLKRDMVRRK
LEKQRRRAS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=31.4814814814815, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=55.4878048780488, Blast_Score=181, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=30.5732484076433, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=30.379746835443, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_XYLF2 (B2I8S4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830539.1
- ProteinModelPortal:   B2I8S4
- SMR:   B2I8S4
- GeneID:   6203139
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1865
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- ProtClustDB:   PRK00150
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19201; Mature: 19069

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILEFPDPRLRTKAVRVGVAEVVSSSFQTLLDDMFETMYAAPGIGLAATQVNVHQR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEHHHHE
FMVIDVSEEKNVPMVFINPEIVTREGDQVFQEGCLSVPGIHADVTRALSIVVRFLDRHGD
EEEEEECCCCCCCEEEECCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EQQLTAEGLLAVCIQHEMDHLDGKLFIDYLSPLKRDMVRRKLEKQRRRAS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLPILEFPDPRLRTKAVRVGVAEVVSSSFQTLLDDMFETMYAAPGIGLAATQVNVHQR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEHHHHE
FMVIDVSEEKNVPMVFINPEIVTREGDQVFQEGCLSVPGIHADVTRALSIVVRFLDRHGD
EEEEEECCCCCCCEEEECCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EQQLTAEGLLAVCIQHEMDHLDGKLFIDYLSPLKRDMVRRKLEKQRRRAS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA