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The map label for this gene is dusA [H]

Identifier: 182682292

GI number: 182682292

Start: 1965831

End: 1966829

Strand: Reverse

Name: dusA [H]

Synonym: XfasM23_1775

Alternate gene names: 182682292

Gene position: 1966829-1965831 (Counterclockwise)

Preceding gene: 182682293

Following gene: 182682291

Centisome position: 77.57

GC content: 51.45

Gene sequence:

>999_bases
GTGATTCCGGTGATTAATCAAGAAAAACAAGGTATATCAGGCGATTTTAGGTTATCTGTCGCCCCGATGATGAATTGGAC
TGATCGCTATTGCCGCGTCTTCCATCGAGTGTTGGCCCCGTCGGCCCGTTTGTACACAGAGATGGTGCATGCCAAAGCAG
TGATTTATGGTGATCGTGAGCGGTTATTGGGTTTTGCGTCGGTTGAGCAGCCGGTTGCGTTGCAATTGGGGGGGAGTGAG
CCAGCATTGCTGGCAAAAGCAGCGAGCATTGCCGTAGATTGGGGGTACAGCGAGATCAATCTCAACTGTGGTTGTCCTTC
TGATCGGGTTCAGGCTGGGTCCTTTGGTGCCTGTCTGATGCGTGAGCCTGCGTTAGTAGCCGATTGTGTGGCGGCGATGG
CAGCCGTAGTTTCAGTTCCAGTAACAGTAAAATGTCGACTGGGGGTGAATGAAGATGATGATTACGGACGTTTTGCTAAG
TTCGTTGACTGGGTGATCGGGGCGTCGAGTAGTAGGATGATTGTGGTACATGCACGTAATGCTTGGTTACAGGGACTTTC
GCCTAAGGAAAATCGTGAAATACCACCGTTACGTTACGATTGGGTTTACCGTTTGAAGCGTGAGTGTCCTGAATTGGCAG
TGGTACTGAATGGCGGTATCACTTCGGTTGAGGCTGGATTGGATCATCTGCTTATGGTCGATGGCGTGATGCTTGGCCGA
GCCGCTTATCAGGATCCTTACATTCTGCACCAATTTTATTGTGCTTTATCTAATGCGCCGGTGCTACCGCGCGCGTTGCT
GCTGCGTGCGTTGCGACCATATGTCGAGGCATGGTTGGAGCAAGGGGTGACGCTCAGGCACATTGTTCGCCATCTGCTTG
GCCTATTTCATGGCCAGCCTGGCGGTCGTGTATTCCGCCAAGTTCTTACCCAAGGTGGGCAAAGATCGGATGCCGACTGG
TCATTGGTGGAACAGGCACTATCAATAATTGAGGGTTAA

Upstream 100 bases:

>100_bases
GCTAGCGTATTGAAGGTTTCAGCTTCTGAATGGACGATTTTACCTTAGAAATACAGAAAACCCTCCAAAAAACCGTAGGG
ATATAAGAGGCTAAAACGCT

Downstream 100 bases:

>100_bases
GAAACATATGCTGCGGTAGTTTGATTAGAGCTTCTCCTCCAATAATATATGGGATTTTTGGCTATACGAATTGTCTTTAG
GTTCTTAAATTGTATTATTT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MIPVINQEKQGISGDFRLSVAPMMNWTDRYCRVFHRVLAPSARLYTEMVHAKAVIYGDRERLLGFASVEQPVALQLGGSE
PALLAKAASIAVDWGYSEINLNCGCPSDRVQAGSFGACLMREPALVADCVAAMAAVVSVPVTVKCRLGVNEDDDYGRFAK
FVDWVIGASSSRMIVVHARNAWLQGLSPKENREIPPLRYDWVYRLKRECPELAVVLNGGITSVEAGLDHLLMVDGVMLGR
AAYQDPYILHQFYCALSNAPVLPRALLLRALRPYVEAWLEQGVTLRHIVRHLLGLFHGQPGGRVFRQVLTQGGQRSDADW
SLVEQALSIIEG

Sequences:

>Translated_332_residues
MIPVINQEKQGISGDFRLSVAPMMNWTDRYCRVFHRVLAPSARLYTEMVHAKAVIYGDRERLLGFASVEQPVALQLGGSE
PALLAKAASIAVDWGYSEINLNCGCPSDRVQAGSFGACLMREPALVADCVAAMAAVVSVPVTVKCRLGVNEDDDYGRFAK
FVDWVIGASSSRMIVVHARNAWLQGLSPKENREIPPLRYDWVYRLKRECPELAVVLNGGITSVEAGLDHLLMVDGVMLGR
AAYQDPYILHQFYCALSNAPVLPRALLLRALRPYVEAWLEQGVTLRHIVRHLLGLFHGQPGGRVFRQVLTQGGQRSDADW
SLVEQALSIIEG
>Mature_332_residues
MIPVINQEKQGISGDFRLSVAPMMNWTDRYCRVFHRVLAPSARLYTEMVHAKAVIYGDRERLLGFASVEQPVALQLGGSE
PALLAKAASIAVDWGYSEINLNCGCPSDRVQAGSFGACLMREPALVADCVAAMAAVVSVPVTVKCRLGVNEDDDYGRFAK
FVDWVIGASSSRMIVVHARNAWLQGLSPKENREIPPLRYDWVYRLKRECPELAVVLNGGITSVEAGLDHLLMVDGVMLGR
AAYQDPYILHQFYCALSNAPVLPRALLLRALRPYVEAWLEQGVTLRHIVRHLLGLFHGQPGGRVFRQVLTQGGQRSDADW
SLVEQALSIIEG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=306, Percent_Identity=26.1437908496732, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI145693211, Length=326, Percent_Identity=51.2269938650307, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1789660, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788462, Length=224, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=253, Percent_Identity=25.296442687747, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=165, Percent_Identity=28.4848484848485, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36654; Mature: 36654

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVINQEKQGISGDFRLSVAPMMNWTDRYCRVFHRVLAPSARLYTEMVHAKAVIYGDRE
CCCCCCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCHH
RLLGFASVEQPVALQLGGSEPALLAKAASIAVDWGYSEINLNCGCPSDRVQAGSFGACLM
HHHHHHCCCCCEEEEECCCCCHHHHHHHHEEEECCCEEEEEECCCCCCCCCCCCCCHHHH
REPALVADCVAAMAAVVSVPVTVKCRLGVNEDDDYGRFAKFVDWVIGASSSRMIVVHARN
CCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
AWLQGLSPKENREIPPLRYDWVYRLKRECPELAVVLNGGITSVEAGLDHLLMVDGVMLGR
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AAYQDPYILHQFYCALSNAPVLPRALLLRALRPYVEAWLEQGVTLRHIVRHLLGLFHGQP
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
GGRVFRQVLTQGGQRSDADWSLVEQALSIIEG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIPVINQEKQGISGDFRLSVAPMMNWTDRYCRVFHRVLAPSARLYTEMVHAKAVIYGDRE
CCCCCCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCHH
RLLGFASVEQPVALQLGGSEPALLAKAASIAVDWGYSEINLNCGCPSDRVQAGSFGACLM
HHHHHHCCCCCEEEEECCCCCHHHHHHHHEEEECCCEEEEEECCCCCCCCCCCCCCHHHH
REPALVADCVAAMAAVVSVPVTVKCRLGVNEDDDYGRFAKFVDWVIGASSSRMIVVHARN
CCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
AWLQGLSPKENREIPPLRYDWVYRLKRECPELAVVLNGGITSVEAGLDHLLMVDGVMLGR
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AAYQDPYILHQFYCALSNAPVLPRALLLRALRPYVEAWLEQGVTLRHIVRHLLGLFHGQP
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
GGRVFRQVLTQGGQRSDADWSLVEQALSIIEG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]