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The map label for this gene is folD [H]

Identifier: 182682055

GI number: 182682055

Start: 1704815

End: 1705690

Strand: Reverse

Name: folD [H]

Synonym: XfasM23_1533

Alternate gene names: 182682055

Gene position: 1705690-1704815 (Counterclockwise)

Preceding gene: 182682056

Following gene: 182682054

Centisome position: 67.27

GC content: 54.57

Gene sequence:

>876_bases
ATGCTGGCAGCCACCGATTTGGCTCGTATTCTCGACGGGCGTCGTATTGCTGATGACCTGCTTGATGCTTTGAAAACACG
CGTGGATGCACGTGTGGCGGCAGGCAAACTGCCTCCAACGTTGGCCGTAGTGCTCGTCGGTTCCGATCCGGCATCAGTGG
TGTATGTCCGCAACAAGCGGCGTGCTGCAGAGAAGGTGGGGATTAAAGCTTATGACTTCGATCTTCCAGAAGCGACGACT
GAGGCTGAGTTGGCCGCGTTGATTGACCGGCTTAATGCCGATCCAAAGATCCACGGTGTCCTGATCCAATTACCATTGCC
GGGCATCCCTGATGCGCATCGATTGATTCAACGTATTGACCCGCGCAAAGATGTGGATGGTTTCCACCCTCAAAATGTTG
GGCACTTAGCATTGCGTGAGTTTGGTCTCAGACCATGCACGCCACGGGGTATTGTCACCTTGCTCGGCCATACCGACCGA
CCGGTCCGAGGGCGTAATGCCACCATTGTTGGAGTGAGCAACCATGTTGGCCGACCGATGGGACTGGAGTTGTTGATGGC
TGGTTGTACTGTCACCAGTTGCCATAAATTCACCCCACCACAGATGTTGGAAGCAGCAGTGCGTCAAGCCGATATTCTGA
TCGTTGCAGTGGGCCGTCCGGGAGTGATTCCTGGAGAGTGGGTGAAGCCAGGTGCAGTCGTGATTGATGTTGGCATTAAT
CGACTTGATGATGGTCAGTTGGTGGGTGATGTTGGTTTTGAGAGTGCAGTTAAGCGTGCTAGCTGGATTACCCCGGTACC
TGGCGGAGTAGGACCGATGACCGTAGCGACGTTGATGCAAAACACCCTGGAAGCCGCCGAAGCGACGGATTGTTGA

Upstream 100 bases:

>100_bases
TCTGACCTTAGGTGGTGATATCGCTTGCAGGGATGTCAGTCATGAAGGAATTGGGCCACAATGGGCGACAAAATATTTTA
CCTTTGGTGTGATCGTATCT

Downstream 100 bases:

>100_bases
TCCCCTAGTTCTGGCATGCGCACGTTGCAAGGTGTCATTAACATAGGCCCTTAGTGTCGATCCGAGAGATTTCGGCGTAA
AATTAAGCATTACCTAAAAC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLAATDLARILDGRRIADDLLDALKTRVDARVAAGKLPPTLAVVLVGSDPASVVYVRNKRRAAEKVGIKAYDFDLPEATT
EAELAALIDRLNADPKIHGVLIQLPLPGIPDAHRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDR
PVRGRNATIVGVSNHVGRPMGLELLMAGCTVTSCHKFTPPQMLEAAVRQADILIVAVGRPGVIPGEWVKPGAVVIDVGIN
RLDDGQLVGDVGFESAVKRASWITPVPGGVGPMTVATLMQNTLEAAEATDC

Sequences:

>Translated_291_residues
MLAATDLARILDGRRIADDLLDALKTRVDARVAAGKLPPTLAVVLVGSDPASVVYVRNKRRAAEKVGIKAYDFDLPEATT
EAELAALIDRLNADPKIHGVLIQLPLPGIPDAHRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDR
PVRGRNATIVGVSNHVGRPMGLELLMAGCTVTSCHKFTPPQMLEAAVRQADILIVAVGRPGVIPGEWVKPGAVVIDVGIN
RLDDGQLVGDVGFESAVKRASWITPVPGGVGPMTVATLMQNTLEAAEATDC
>Mature_291_residues
MLAATDLARILDGRRIADDLLDALKTRVDARVAAGKLPPTLAVVLVGSDPASVVYVRNKRRAAEKVGIKAYDFDLPEATT
EAELAALIDRLNADPKIHGVLIQLPLPGIPDAHRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDR
PVRGRNATIVGVSNHVGRPMGLELLMAGCTVTSCHKFTPPQMLEAAVRQADILIVAVGRPGVIPGEWVKPGAVVIDVGIN
RLDDGQLVGDVGFESAVKRASWITPVPGGVGPMTVATLMQNTLEAAEATDC

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=44.3298969072165, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI94721354, Length=300, Percent_Identity=42.6666666666667, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.6621160409556, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI36796743, Length=203, Percent_Identity=24.6305418719212, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=60.2150537634409, Blast_Score=330, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=39.3835616438356, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=41.8060200668896, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=43.205574912892, Blast_Score=201, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=25.6944444444444, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=47.4916387959866, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=48.122866894198, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=42.2145328719723, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=42.2145328719723, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=42.2145328719723, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=42.2145328719723, Blast_Score=205, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31002; Mature: 31002

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAATDLARILDGRRIADDLLDALKTRVDARVAAGKLPPTLAVVLVGSDPASVVYVRNKR
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCH
RAAEKVGIKAYDFDLPEATTEAELAALIDRLNADPKIHGVLIQLPLPGIPDAHRLIQRID
HHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCC
PRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDRPVRGRNATIVGVSNHVGRPM
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCC
GLELLMAGCTVTSCHKFTPPQMLEAAVRQADILIVAVGRPGVIPGEWVKPGAVVIDVGIN
CHHHHHHCCCEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCC
RLDDGQLVGDVGFESAVKRASWITPVPGGVGPMTVATLMQNTLEAAEATDC
CCCCCCEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAATDLARILDGRRIADDLLDALKTRVDARVAAGKLPPTLAVVLVGSDPASVVYVRNKR
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCH
RAAEKVGIKAYDFDLPEATTEAELAALIDRLNADPKIHGVLIQLPLPGIPDAHRLIQRID
HHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCC
PRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDRPVRGRNATIVGVSNHVGRPM
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCC
GLELLMAGCTVTSCHKFTPPQMLEAAVRQADILIVAVGRPGVIPGEWVKPGAVVIDVGIN
CHHHHHHCCCEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCC
RLDDGQLVGDVGFESAVKRASWITPVPGGVGPMTVATLMQNTLEAAEATDC
CCCCCCEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]