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The map label for this gene is glpF [H]

Identifier: 182681920

GI number: 182681920

Start: 1543653

End: 1544480

Strand: Direct

Name: glpF [H]

Synonym: XfasM23_1388

Alternate gene names: 182681920

Gene position: 1543653-1544480 (Clockwise)

Preceding gene: 182681919

Following gene: 182681921

Centisome position: 60.88

GC content: 55.68

Gene sequence:

>828_bases
ATGAACCGAAAGATGTTTGGCGAATTAATTTCCGAAGCAATCGCAATGTTCATCATCATCGCATTGGGCGAATCTGCGGC
CGCAATGTACATCCTTTACGACCCCAGTCCTTATCAAAACGCCTACTGGGGCCTATGTATCTGCTGGGGATTGGCGGTGA
CAATCGCCATCTACGTCACCGCTTCGGTTTCCGGAACCCATGCCAATCCGGCCGTTACCTTGGCACTGGCGCTCTACCGC
GGCTTCCCTTGGAAAAAAGTATTGCCGTACTGGGCAGCACAAGTCATCGGCGCCTTCATCGGTGCAATGATTGTGTACCA
GCTCTATAGCCCTGTCATCGACTACTACAACCACATTCACCAACTCACCCGAGACGCTGGCGGGGCTGCTGGCGTGTTTT
TCACTTCCACAGGGATGGCAATCACCCCCATACACGCACTCGGTGATGAAATCATTCTGACCGCATTCCTGGTCTTCGGC
ATTTTCGCAATCACCGAACGCTTCAACGAAGCAGCGCCCACCGCCAACAGCGGCGCACTGGTCATCGGACTCCTCGTAGC
AACCATCGGTGCCTGCATGGGCTACCTAGAAGGATGGGCCATCAACCCAGCACGCGATCTCGGCCCACGTCTGTTCGCCT
TTCTGGCGGGCTGGGGTGAATCGGCATTCCCCGGAAAAGATCACTACTGGTGGATTCCGATTATCGGCCCCCTGATCGGC
GGCGTGATGGGTGCAACCGCATTCCAATGCCTGATCTATCCATTCCTACCGGCACGGGTACAGGCCAAACAACAAGCCGC
GATACTGCTGCACAGGAAGCATCCCTGA

Upstream 100 bases:

>100_bases
ACATCAATTGCACTGCCCCTGAAGCTAAGACGTTTCAGCCACCACGGAATGGAGATAAATGCACTCAATCCCCCTGCCCC
CTGCCTGAAGGAACACCGCA

Downstream 100 bases:

>100_bases
ATCATTCCCCTTCACCAAGACCACGCACCCTGTCCGTATCGAAAACACTGCACTGATGAAAAAAAAATACATACTGGCCA
TTGACCAAGGCACCACCAGC

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNRKMFGELISEAIAMFIIIALGESAAAMYILYDPSPYQNAYWGLCICWGLAVTIAIYVTASVSGTHANPAVTLALALYR
GFPWKKVLPYWAAQVIGAFIGAMIVYQLYSPVIDYYNHIHQLTRDAGGAAGVFFTSTGMAITPIHALGDEIILTAFLVFG
IFAITERFNEAAPTANSGALVIGLLVATIGACMGYLEGWAINPARDLGPRLFAFLAGWGESAFPGKDHYWWIPIIGPLIG
GVMGATAFQCLIYPFLPARVQAKQQAAILLHRKHP

Sequences:

>Translated_275_residues
MNRKMFGELISEAIAMFIIIALGESAAAMYILYDPSPYQNAYWGLCICWGLAVTIAIYVTASVSGTHANPAVTLALALYR
GFPWKKVLPYWAAQVIGAFIGAMIVYQLYSPVIDYYNHIHQLTRDAGGAAGVFFTSTGMAITPIHALGDEIILTAFLVFG
IFAITERFNEAAPTANSGALVIGLLVATIGACMGYLEGWAINPARDLGPRLFAFLAGWGESAFPGKDHYWWIPIIGPLIG
GVMGATAFQCLIYPFLPARVQAKQQAAILLHRKHP
>Mature_275_residues
MNRKMFGELISEAIAMFIIIALGESAAAMYILYDPSPYQNAYWGLCICWGLAVTIAIYVTASVSGTHANPAVTLALALYR
GFPWKKVLPYWAAQVIGAFIGAMIVYQLYSPVIDYYNHIHQLTRDAGGAAGVFFTSTGMAITPIHALGDEIILTAFLVFG
IFAITERFNEAAPTANSGALVIGLLVATIGACMGYLEGWAINPARDLGPRLFAFLAGWGESAFPGKDHYWWIPIIGPLIG
GVMGATAFQCLIYPFLPARVQAKQQAAILLHRKHP

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=272, Percent_Identity=34.1911764705882, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI4502187, Length=257, Percent_Identity=32.6848249027237, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI22538420, Length=265, Percent_Identity=30.9433962264151, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI157266307, Length=255, Percent_Identity=35.2941176470588, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI310133356, Length=209, Percent_Identity=33.0143540669856, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI310114181, Length=209, Percent_Identity=33.0143540669856, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4755125, Length=225, Percent_Identity=29.7777777777778, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502181, Length=225, Percent_Identity=29.7777777777778, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502179, Length=268, Percent_Identity=27.6119402985075, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI6912506, Length=261, Percent_Identity=27.9693486590038, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI86792455, Length=255, Percent_Identity=28.2352941176471, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790362, Length=264, Percent_Identity=39.3939393939394, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17544068, Length=232, Percent_Identity=36.6379310344828, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71992966, Length=253, Percent_Identity=33.201581027668, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994009, Length=252, Percent_Identity=30.1587301587302, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531431, Length=263, Percent_Identity=29.6577946768061, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=263, Percent_Identity=29.6577946768061, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71992961, Length=254, Percent_Identity=31.1023622047244, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17533613, Length=241, Percent_Identity=29.8755186721992, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32564052, Length=241, Percent_Identity=29.8755186721992, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321054, Length=184, Percent_Identity=40.2173913043478, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322985, Length=215, Percent_Identity=33.4883720930233, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24762342, Length=248, Percent_Identity=29.8387096774194, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550503, Length=248, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24762344, Length=198, Percent_Identity=29.7979797979798, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24762346, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24762348, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20130305, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29777; Mature: 29777

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKMFGELISEAIAMFIIIALGESAAAMYILYDPSPYQNAYWGLCICWGLAVTIAIYVT
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHE
ASVSGTHANPAVTLALALYRGFPWKKVLPYWAAQVIGAFIGAMIVYQLYSPVIDYYNHIH
ECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTRDAGGAAGVFFTSTGMAITPIHALGDEIILTAFLVFGIFAITERFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VIGLLVATIGACMGYLEGWAINPARDLGPRLFAFLAGWGESAFPGKDHYWWIPIIGPLIG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHH
GVMGATAFQCLIYPFLPARVQAKQQAAILLHRKHP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MNRKMFGELISEAIAMFIIIALGESAAAMYILYDPSPYQNAYWGLCICWGLAVTIAIYVT
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHE
ASVSGTHANPAVTLALALYRGFPWKKVLPYWAAQVIGAFIGAMIVYQLYSPVIDYYNHIH
ECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTRDAGGAAGVFFTSTGMAITPIHALGDEIILTAFLVFGIFAITERFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VIGLLVATIGACMGYLEGWAINPARDLGPRLFAFLAGWGESAFPGKDHYWWIPIIGPLIG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHH
GVMGATAFQCLIYPFLPARVQAKQQAAILLHRKHP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]