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The map label for this gene is glpK

Identifier: 182681921

GI number: 182681921

Start: 1544536

End: 1546035

Strand: Direct

Name: glpK

Synonym: XfasM23_1389

Alternate gene names: 182681921

Gene position: 1544536-1546035 (Clockwise)

Preceding gene: 182681920

Following gene: 182681923

Centisome position: 60.91

GC content: 56.67

Gene sequence:

>1500_bases
ATGAAAAAAAAATACATACTGGCCATTGACCAAGGCACCACCAGCTCCCGGGCCATTCTGTTTGACCACAAAGGCCGCAT
CATCGGGATGGCACAGCGGGAATTCACCCAGATATTTCCCCAACCTGGCTGGGTCGAACACAACCCGCGCGACATCATGA
CCAGTGTGTACACCACGATCACCGAACTGCTCAACAACACCCAGATTGACGTGCGCGCCATCGCCGGAATCGGTATTACC
AACCAACGCGAAACAACCGTCATCTGGGATCGTCAGACCGGCCAACCGATCTACAACGCAATCGTTTGGCAATCACGCCA
AACCAAGGACATCTGCGACCAACTCACGACCGCCGGGTACCAAGATATGGTTCACGCCAAAACCGGACTCCTGATCGACG
CCTATTTTTCAGGCACCAAGGTGAAATGGATTCTGGACCATGTCGAAAACGCACATACACAAGCAGCGCGCGGAGAACTG
GCATTCGGCACCATCGACACCTGGATCATCTGGAACCTGACCGGCGGCCAAGTCCATGTCACCGATTACAGCAACGCCTC
ACGCACCCTGCTGTACGACATCCACGCCCTGCGTTGGGATCCAGACCTGCTGACAATGCTAGACATACCAGCGGCGATCC
TGCCGGACGTCCGTTCATCCAGCGAAATTTACGGTCTCACCCAAACCCCGTATTTCCACGGAGAACAGATACCGATTGCC
GGAATCGCCGGCGATCAGCAAGCCGCACTCTTCGGCCAAGCCTGTTTCGAACCTGGCATGGCCAAAAACACCTACGGCAC
CGGCTGTTTCATGCTCATGCACACCGGCAAAAAAGCCGTGGAATCCCAAAATGGACTACTCACTACGATCGCTTGGGGCC
TGAATGGAGAAATCGAGTACGCACTAGAAGGCTCGATCTTCATCGCTGGCTCTGTGGTGCAATGGCTGCGCGATGGATTA
CGCATGTTCGGCAAAGCCAGCGATTCACAAGCCTACGCCGACCGTGTCAGCGACAACGGCGGCGTATACGTTGTACCGGC
CTTCGTTGGCCTGGGTGCGCCCTACTGGCGTAGCGATGTCCGCGGCGCAGTGTTCGGACTGACACGCAGCACCACCAAAG
AACACTTCGTCCGCGCCGCACTAGAATCAATGGCCTACCAAACCCGCGACGTCCTGAGCGCAATGCAGGCAGATGCAGAC
ATCGAACTCAAAGAACTACGCACTGACGGCGCGGCCATCACCAACGACTTCATGGCCCAATTCCAGAGCGATATCCTCGC
CGTGCCAGTGCTGCGCTCGCAGATCGCCGAAACCACAGCACTCGGTGCCGCCTACCTGGCTGGACTTGCGACCGGATTTT
GGTCCAGCCGCGAAGAGATGACGCAACACTGGGCGATCAATCGCTGCTTCAAGCCACAGATGGATAAGGAACAACGCGAG
CACCTATACGCAGGCTGGAAACAGGCCGTCGAAGCCACATTAGGTTTCCGTGTGGCCTGA

Upstream 100 bases:

>100_bases
GCCAAACAACAAGCCGCGATACTGCTGCACAGGAAGCATCCCTGAATCATTCCCCTTCACCAAGACCACGCACCCTGTCC
GTATCGAAAACACTGCACTG

Downstream 100 bases:

>100_bases
ACGCGGCAGTGGCAGCGACATCACCAACACGTATCCCTTTTCTCAGACCACCCTGCACCAGCGCATCACATCCACAGCGA
CATACAACGCCCGAGCCACA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKKKYILAIDQGTTSSRAILFDHKGRIIGMAQREFTQIFPQPGWVEHNPRDIMTSVYTTITELLNNTQIDVRAIAGIGIT
NQRETTVIWDRQTGQPIYNAIVWQSRQTKDICDQLTTAGYQDMVHAKTGLLIDAYFSGTKVKWILDHVENAHTQAARGEL
AFGTIDTWIIWNLTGGQVHVTDYSNASRTLLYDIHALRWDPDLLTMLDIPAAILPDVRSSSEIYGLTQTPYFHGEQIPIA
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMHTGKKAVESQNGLLTTIAWGLNGEIEYALEGSIFIAGSVVQWLRDGL
RMFGKASDSQAYADRVSDNGGVYVVPAFVGLGAPYWRSDVRGAVFGLTRSTTKEHFVRAALESMAYQTRDVLSAMQADAD
IELKELRTDGAAITNDFMAQFQSDILAVPVLRSQIAETTALGAAYLAGLATGFWSSREEMTQHWAINRCFKPQMDKEQRE
HLYAGWKQAVEATLGFRVA

Sequences:

>Translated_499_residues
MKKKYILAIDQGTTSSRAILFDHKGRIIGMAQREFTQIFPQPGWVEHNPRDIMTSVYTTITELLNNTQIDVRAIAGIGIT
NQRETTVIWDRQTGQPIYNAIVWQSRQTKDICDQLTTAGYQDMVHAKTGLLIDAYFSGTKVKWILDHVENAHTQAARGEL
AFGTIDTWIIWNLTGGQVHVTDYSNASRTLLYDIHALRWDPDLLTMLDIPAAILPDVRSSSEIYGLTQTPYFHGEQIPIA
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMHTGKKAVESQNGLLTTIAWGLNGEIEYALEGSIFIAGSVVQWLRDGL
RMFGKASDSQAYADRVSDNGGVYVVPAFVGLGAPYWRSDVRGAVFGLTRSTTKEHFVRAALESMAYQTRDVLSAMQADAD
IELKELRTDGAAITNDFMAQFQSDILAVPVLRSQIAETTALGAAYLAGLATGFWSSREEMTQHWAINRCFKPQMDKEQRE
HLYAGWKQAVEATLGFRVA
>Mature_499_residues
MKKKYILAIDQGTTSSRAILFDHKGRIIGMAQREFTQIFPQPGWVEHNPRDIMTSVYTTITELLNNTQIDVRAIAGIGIT
NQRETTVIWDRQTGQPIYNAIVWQSRQTKDICDQLTTAGYQDMVHAKTGLLIDAYFSGTKVKWILDHVENAHTQAARGEL
AFGTIDTWIIWNLTGGQVHVTDYSNASRTLLYDIHALRWDPDLLTMLDIPAAILPDVRSSSEIYGLTQTPYFHGEQIPIA
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMHTGKKAVESQNGLLTTIAWGLNGEIEYALEGSIFIAGSVVQWLRDGL
RMFGKASDSQAYADRVSDNGGVYVVPAFVGLGAPYWRSDVRGAVFGLTRSTTKEHFVRAALESMAYQTRDVLSAMQADAD
IELKELRTDGAAITNDFMAQFQSDILAVPVLRSQIAETTALGAAYLAGLATGFWSSREEMTQHWAINRCFKPQMDKEQRE
HLYAGWKQAVEATLGFRVA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=45.2191235059761, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=45.2191235059761, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=45.2191235059761, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=44.8616600790514, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=30.5882352941176, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=58.989898989899, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=524, Percent_Identity=22.5190839694656, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787789, Length=491, Percent_Identity=22.4032586558045, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789168, Length=497, Percent_Identity=22.9376257545272, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789987, Length=478, Percent_Identity=22.5941422594142, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=40.7554671968191, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=528, Percent_Identity=25.5681818181818, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=31.7164179104478, Blast_Score=141, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=35.7400722021661, Blast_Score=300, Evalue=4e-82,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=41.5204678362573, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=40.1197604790419, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=40.1197604790419, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=40.1197604790419, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=40.1197604790419, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=40.1197604790419, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=439, Percent_Identity=43.0523917995444, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=33.0152671755725, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=33.6048879837067, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=29.2682926829268, Blast_Score=224, Evalue=9e-59,
Organism=Drosophila melanogaster, GI21357867, Length=513, Percent_Identity=29.0448343079922, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656372, Length=338, Percent_Identity=31.6568047337278, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45552909, Length=293, Percent_Identity=31.0580204778157, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_XYLF2 (B2I618)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830081.1
- ProteinModelPortal:   B2I618
- SMR:   B2I618
- GeneID:   6202318
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1389
- HOGENOM:   HBG511469
- OMA:   IQRSADV
- ProtClustDB:   PRK00047
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55343; Mature: 55343

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 245-245 BINDING 267-267 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKYILAIDQGTTSSRAILFDHKGRIIGMAQREFTQIFPQPGWVEHNPRDIMTSVYTTI
CCCEEEEEEECCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TELLNNTQIDVRAIAGIGITNQRETTVIWDRQTGQPIYNAIVWQSRQTKDICDQLTTAGY
HHHHCCCCEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCH
QDMVHAKTGLLIDAYFSGTKVKWILDHVENAHTQAARGELAFGTIDTWIIWNLTGGQVHV
HHHHHHHCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECEEEEEEECCCEEEE
TDYSNASRTLLYDIHALRWDPDLLTMLDIPAAILPDVRSSSEIYGLTQTPYFHGEQIPIA
EECCCCCCEEEEEEEHEEECCCHHHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEE
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMHTGKKAVESQNGLLTTIAWGLNGEIEY
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEE
ALEGSIFIAGSVVQWLRDGLRMFGKASDSQAYADRVSDNGGVYVVPAFVGLGAPYWRSDV
EECCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEHHHHCCCCHHHHCC
RGAVFGLTRSTTKEHFVRAALESMAYQTRDVLSAMQADADIELKELRTDGAAITNDFMAQ
CHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHH
FQSDILAVPVLRSQIAETTALGAAYLAGLATGFWSSREEMTQHWAINRCFKPQMDKEQRE
HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHH
HLYAGWKQAVEATLGFRVA
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKKYILAIDQGTTSSRAILFDHKGRIIGMAQREFTQIFPQPGWVEHNPRDIMTSVYTTI
CCCEEEEEEECCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TELLNNTQIDVRAIAGIGITNQRETTVIWDRQTGQPIYNAIVWQSRQTKDICDQLTTAGY
HHHHCCCCEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCH
QDMVHAKTGLLIDAYFSGTKVKWILDHVENAHTQAARGELAFGTIDTWIIWNLTGGQVHV
HHHHHHHCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECEEEEEEECCCEEEE
TDYSNASRTLLYDIHALRWDPDLLTMLDIPAAILPDVRSSSEIYGLTQTPYFHGEQIPIA
EECCCCCCEEEEEEEHEEECCCHHHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEE
GIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMHTGKKAVESQNGLLTTIAWGLNGEIEY
EECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEE
ALEGSIFIAGSVVQWLRDGLRMFGKASDSQAYADRVSDNGGVYVVPAFVGLGAPYWRSDV
EECCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEHHHHCCCCHHHHCC
RGAVFGLTRSTTKEHFVRAALESMAYQTRDVLSAMQADADIELKELRTDGAAITNDFMAQ
CHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHH
FQSDILAVPVLRSQIAETTALGAAYLAGLATGFWSSREEMTQHWAINRCFKPQMDKEQRE
HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHH
HLYAGWKQAVEATLGFRVA
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA