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The map label for this gene is birA [H]

Identifier: 182681678

GI number: 182681678

Start: 1282988

End: 1283941

Strand: Direct

Name: birA [H]

Synonym: XfasM23_1136

Alternate gene names: 182681678

Gene position: 1282988-1283941 (Clockwise)

Preceding gene: 182681677

Following gene: 182681679

Centisome position: 50.6

GC content: 50.73

Gene sequence:

>954_bases
GTGGACGAGCGTGTACTGCTGACCTGCTTGGGGTGTAGTTATGTCTCTGGCAATGTTTTGGCCAGCATGACTGGAATGAC
AGAGGCTACAATTGCGTCGTGTATTCAAGCACTTCGTGCCGTCGGCATTCCTATCAAGTCACGTGCAGAGCAGGGGTATG
CTTTAGCTGCACCGTTATCCTTACTGACTGCTGAGCGTATCCGTGCAGAGTTATCCCACTCTATTGCTGCCGAGCTTCGT
GTGTTGGATGTGGCTTGGACGCTTGAATCAACAAATACTTCCTTGTTAGCACGTCCGGCGTTGGCCGATGGTGTAGAAGT
ATTGCTGGCAGAGCGTCAGACGGGTGGTCGCGGCCAGCGTGGTAGGAGCTGGATTTCTCCATTAGCAGCAAATATTTATT
TGTCATTGTCAAGAGTTTTTTCTGGCGGCGCGTCACAAGTGATGGGGTTGAGCGTGGCGGTTGGCGTAGCAATTGCTGAA
GCATTAATTGCCGCTGGCTTCTCCCACGTCCGACTAAAATGGCCTAATGATCTCGTTGATAATGGTCGGAAGCTTGGCGG
CGTGTTAGTTGAACGACAGGGAGTGGCACGTGCAGTGATCGGATTAGGGTTGAATGTGCGGATGCCAAAAAATTTTGCTG
AGCAGATTGACCAGCCTTGGGTCGATTTGGAGACTCTTGCGGGCAGTGCAGTAGAGCGTAATGCATTGGTTGCACTATTG
TTGTCGGCATTGCTGCCTGCACTACATTTGTTTCAACTTGAGGGCTTGGCACCGTTTTTGCCGCGCTATGCCAAGTTGGA
TTGTCTTGTTGGGTGTGCGGTATGGATTGAAGAGGCTAATCTAAAATATGTTGGTATTGCGCGTGGTTTGGCCGCCGATG
GTGCACTCTTGGTAGAAATTAACGGCGTGTTGCGTCCTTTTCATACTGGCCAAGTTTCGGTCAGACCGATATGA

Upstream 100 bases:

>100_bases
GATTTTCGAAAAATATCTAATTTGATGTAGTTTTCAAATTTTGTTTGAGCACCCTTGGCAGCATCTCGCATAATGGTGAT
TCCGTGGTTGGAAATTTAAT

Downstream 100 bases:

>100_bases
ATGATTGGTTATTCGATCTAGGTAATTCGCGTTTTAAATGTGCATCTCTCAGGGAGGGTGTGATTGGTCCTGTAACGGTT
TTGCCGTACTTAACAGAAAC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDERVLLTCLGCSYVSGNVLASMTGMTEATIASCIQALRAVGIPIKSRAEQGYALAAPLSLLTAERIRAELSHSIAAELR
VLDVAWTLESTNTSLLARPALADGVEVLLAERQTGGRGQRGRSWISPLAANIYLSLSRVFSGGASQVMGLSVAVGVAIAE
ALIAAGFSHVRLKWPNDLVDNGRKLGGVLVERQGVARAVIGLGLNVRMPKNFAEQIDQPWVDLETLAGSAVERNALVALL
LSALLPALHLFQLEGLAPFLPRYAKLDCLVGCAVWIEEANLKYVGIARGLAADGALLVEINGVLRPFHTGQVSVRPI

Sequences:

>Translated_317_residues
MDERVLLTCLGCSYVSGNVLASMTGMTEATIASCIQALRAVGIPIKSRAEQGYALAAPLSLLTAERIRAELSHSIAAELR
VLDVAWTLESTNTSLLARPALADGVEVLLAERQTGGRGQRGRSWISPLAANIYLSLSRVFSGGASQVMGLSVAVGVAIAE
ALIAAGFSHVRLKWPNDLVDNGRKLGGVLVERQGVARAVIGLGLNVRMPKNFAEQIDQPWVDLETLAGSAVERNALVALL
LSALLPALHLFQLEGLAPFLPRYAKLDCLVGCAVWIEEANLKYVGIARGLAADGALLVEINGVLRPFHTGQVSVRPI
>Mature_317_residues
MDERVLLTCLGCSYVSGNVLASMTGMTEATIASCIQALRAVGIPIKSRAEQGYALAAPLSLLTAERIRAELSHSIAAELR
VLDVAWTLESTNTSLLARPALADGVEVLLAERQTGGRGQRGRSWISPLAANIYLSLSRVFSGGASQVMGLSVAVGVAIAE
ALIAAGFSHVRLKWPNDLVDNGRKLGGVLVERQGVARAVIGLGLNVRMPKNFAEQIDQPWVDLETLAGSAVERNALVALL
LSALLPALHLFQLEGLAPFLPRYAKLDCLVGCAVWIEEANLKYVGIARGLAADGALLVEINGVLRPFHTGQVSVRPI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=307, Percent_Identity=39.0879478827362, Blast_Score=205, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 33574; Mature: 33574

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERVLLTCLGCSYVSGNVLASMTGMTEATIASCIQALRAVGIPIKSRAEQGYALAAPLS
CCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHH
LLTAERIRAELSHSIAAELRVLDVAWTLESTNTSLLARPALADGVEVLLAERQTGGRGQR
HHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCEEECCCHHCCHHHHHHHCCCCCCCCC
GRSWISPLAANIYLSLSRVFSGGASQVMGLSVAVGVAIAEALIAAGFSHVRLKWPNDLVD
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
NGRKLGGVLVERQGVARAVIGLGLNVRMPKNFAEQIDQPWVDLETLAGSAVERNALVALL
CCHHHCCEEEECCCHHHHHHHCCCEEECCHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHH
LSALLPALHLFQLEGLAPFLPRYAKLDCLVGCAVWIEEANLKYVGIARGLAADGALLVEI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEE
NGVLRPFHTGQVSVRPI
CCCCCCCCCCEEEEECC
>Mature Secondary Structure
MDERVLLTCLGCSYVSGNVLASMTGMTEATIASCIQALRAVGIPIKSRAEQGYALAAPLS
CCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHH
LLTAERIRAELSHSIAAELRVLDVAWTLESTNTSLLARPALADGVEVLLAERQTGGRGQR
HHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCEEECCCHHCCHHHHHHHCCCCCCCCC
GRSWISPLAANIYLSLSRVFSGGASQVMGLSVAVGVAIAEALIAAGFSHVRLKWPNDLVD
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
NGRKLGGVLVERQGVARAVIGLGLNVRMPKNFAEQIDQPWVDLETLAGSAVERNALVALL
CCHHHCCEEEECCCHHHHHHHCCCEEECCHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHH
LSALLPALHLFQLEGLAPFLPRYAKLDCLVGCAVWIEEANLKYVGIARGLAADGALLVEI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEE
NGVLRPFHTGQVSVRPI
CCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]