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The map label for this gene is nfi [H]

Identifier: 182681500

GI number: 182681500

Start: 1118463

End: 1119251

Strand: Direct

Name: nfi [H]

Synonym: XfasM23_0954

Alternate gene names: 182681500

Gene position: 1118463-1119251 (Clockwise)

Preceding gene: 182681498

Following gene: 182681502

Centisome position: 44.11

GC content: 50.32

Gene sequence:

>789_bases
ATGATTGTAACGTCAGGTCGAGATTGCCTGTATACGCTCTTAATATCTGATATAGGCATGATGGTATTTATGAAGATTTC
ATCCATTGATTCTATCTTTGCAGCCTGGGATGGCAGTATTACCGAGGCGCGACGCTTGCAAAGCGATATGGCCGAACGCA
TTGTGCTTAAGGACGAGCCTAATCTATTGTCTGAACCAACATTACTGGCTGGTTTTGACGTTGGCTTTGAGGACGAAGGA
CGCACGACACGGGCTGCTGCTGTTTTAATGAATGCAGGCGATTTAAGGTTGTTAGAAACCCATGTCGTACGTGTACCAAC
GTCAATGCCCTATGTGCCTGGACTCCTCAGCTTTCGCGAGTTGCCTGCACTCCTGCAAGCTCTGACCCAATTGTCACGAA
TTCCAGCACTTGTATTTGTAGATGGACATGGAATCGCGCATCCACGTCGGCTCGGGATCGCCGCGCATTTTGGCTTAGTG
ACCAACCTACCTTGTATCGGCGTAGCTAAAAAGCGCCTCGTTGGCGACTTTGTCGAACCAGGTACCGCCTTCGGTGAGCA
CACTCCGATCCTATTACATGGGACCCAGGTTGGCTGGGCATTGCGCAGCAAGATGCACTGCAAACCGCTGATTATTTCAC
CAGGCCATAAAGTTTCACTTCATAGCGCGCTTACATGGACCCAGCGCTGTCTTACCGGCTATCGTCTACCAGAACCCACT
CGGCAAGCGGATCGTCTGGCATCGCGACGTGGTCAAAAAATCGTCAGTGACTTACCGTCCCTACTATAA

Upstream 100 bases:

>100_bases
GCCAGTAAAACGGTTCATTGAGTTCCATTGACTCTGTCCATGGCGTAGCAATACAAGTTTACGAGTCATGCGGCTTTCCT
GAATTGAAAGTATCTAAAAC

Downstream 100 bases:

>100_bases
AATACCCCTGGTGAAAAAATATCTTAGGTATCCACAACACAACACCATTTCATTACGCGTTTTACCTGTGATTTGTAACG
ATGCCGGTACTCACACCTAC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIVTSGRDCLYTLLISDIGMMVFMKISSIDSIFAAWDGSITEARRLQSDMAERIVLKDEPNLLSEPTLLAGFDVGFEDEG
RTTRAAAVLMNAGDLRLLETHVVRVPTSMPYVPGLLSFRELPALLQALTQLSRIPALVFVDGHGIAHPRRLGIAAHFGLV
TNLPCIGVAKKRLVGDFVEPGTAFGEHTPILLHGTQVGWALRSKMHCKPLIISPGHKVSLHSALTWTQRCLTGYRLPEPT
RQADRLASRRGQKIVSDLPSLL

Sequences:

>Translated_262_residues
MIVTSGRDCLYTLLISDIGMMVFMKISSIDSIFAAWDGSITEARRLQSDMAERIVLKDEPNLLSEPTLLAGFDVGFEDEG
RTTRAAAVLMNAGDLRLLETHVVRVPTSMPYVPGLLSFRELPALLQALTQLSRIPALVFVDGHGIAHPRRLGIAAHFGLV
TNLPCIGVAKKRLVGDFVEPGTAFGEHTPILLHGTQVGWALRSKMHCKPLIISPGHKVSLHSALTWTQRCLTGYRLPEPT
RQADRLASRRGQKIVSDLPSLL
>Mature_262_residues
MIVTSGRDCLYTLLISDIGMMVFMKISSIDSIFAAWDGSITEARRLQSDMAERIVLKDEPNLLSEPTLLAGFDVGFEDEG
RTTRAAAVLMNAGDLRLLETHVVRVPTSMPYVPGLLSFRELPALLQALTQLSRIPALVFVDGHGIAHPRRLGIAAHFGLV
TNLPCIGVAKKRLVGDFVEPGTAFGEHTPILLHGTQVGWALRSKMHCKPLIISPGHKVSLHSALTWTQRCLTGYRLPEPT
RQADRLASRRGQKIVSDLPSLL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=225, Percent_Identity=35.5555555555556, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI257467548, Length=218, Percent_Identity=32.5688073394495, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI257467550, Length=218, Percent_Identity=32.5688073394495, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI87082357, Length=207, Percent_Identity=54.1062801932367, Blast_Score=226, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 28705; Mature: 28705

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTSGRDCLYTLLISDIGMMVFMKISSIDSIFAAWDGSITEARRLQSDMAERIVLKDEP
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCC
NLLSEPTLLAGFDVGFEDEGRTTRAAAVLMNAGDLRLLETHVVRVPTSMPYVPGLLSFRE
CCCCCCCEEEEECCCCCCCCCHHHHHHEEECCCCEEEEEHHEEECCCCCCCCCHHHHHHH
LPALLQALTQLSRIPALVFVDGHGIAHPRRLGIAAHFGLVTNLPCIGVAKKRLVGDFVEP
HHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
GTAFGEHTPILLHGTQVGWALRSKMHCKPLIISPGHKVSLHSALTWTQRCLTGYRLPEPT
CCCCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCH
RQADRLASRRGQKIVSDLPSLL
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVTSGRDCLYTLLISDIGMMVFMKISSIDSIFAAWDGSITEARRLQSDMAERIVLKDEP
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCC
NLLSEPTLLAGFDVGFEDEGRTTRAAAVLMNAGDLRLLETHVVRVPTSMPYVPGLLSFRE
CCCCCCCEEEEECCCCCCCCCHHHHHHEEECCCCEEEEEHHEEECCCCCCCCCHHHHHHH
LPALLQALTQLSRIPALVFVDGHGIAHPRRLGIAAHFGLVTNLPCIGVAKKRLVGDFVEP
HHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
GTAFGEHTPILLHGTQVGWALRSKMHCKPLIISPGHKVSLHSALTWTQRCLTGYRLPEPT
CCCCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCH
RQADRLASRRGQKIVSDLPSLL
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]