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The map label for this gene is traC [H]

Identifier: 182681385

GI number: 182681385

Start: 986870

End: 987943

Strand: Reverse

Name: traC [H]

Synonym: XfasM23_0834

Alternate gene names: 182681385

Gene position: 987943-986870 (Counterclockwise)

Preceding gene: 182681386

Following gene: 182681380

Centisome position: 38.96

GC content: 51.4

Gene sequence:

>1074_bases
ATGAGTCGGCAAGTTCCGGCACTTTCTCCAGAAAAAGAATTTGCAGAGACGTTACGAAGCATGGGATGTCTCGTGAGTGG
TGAACATCCCTTCATGGACGCTAAGAAATACCGTATTGAGGTCGAAGGAGACAAAAAGGGGGGAAAGGCTGGTTTTTATG
TCGCTTACCTCGACGGTCACCCATACGGCTATATCAAGAACAACCGTACTGGCATCGAATTGAAGTGGAAAAGCAAAGGG
TACTCGCTTGATCCAGAGCAAAAGGCCGCAATGCAAGCGGAGGCAACCGCAAAATTGGCTGCACGCGCAGAGCAGCAGCA
GCAAGAACAAGAGGCTGTCGCGCAACATGTCGTAAAGCAGATGGATGATCTTGTGACTGTTATCGAGCCGACGCCCTATC
TAAAAGCTAAAGGCATTACGCCACAGGCTGGTATATACACTGACAGTGATGGCCAGAAGACCTACATTCCAGCTATCGAT
TCGGATGGTAAGCAATGGACGATGCAATACATCCAAGAGGACGGTACAAAGCGATTTGCAAAAGGGGGGCGTAAGGAGGG
TTGTTTTCACCCCATAGGGGGTCTTGCTGCTATCGCAGCAGCGCCCGTGATCGTCATTGGCCAGGGCTATGCCACTGTGG
CGCAAGTGTCTCAGGCATTGGGTTATGGGACCGTTGCCGCATTTGATGCTGGCAATCTGGAAGGTGTTGCCAAAGCATTG
CACAAGTCTTTCCCAGACAAGCCTATTCTGATTGCCGGTGACGATGATCAGTCTTTACCTAACAATCCAGGAAGGACCAA
GGCTCAAGAGGCAGCGCGTGCTGTAGACGGTACGGCGTTCTTCCCAATCTTCGCGCCAGGTGAACAGGCGGATAATCCGA
AAGGCTTCACCGATTTCAATGATCTTGCCACTAAGAGTGCGCTCGGTATCGAAGCGGTTGAACGGCAGGTACATGCGGCT
GTTAGCAAGGCTATCGATAATCATCAGAAACGTGTTCAGAAGCAGACTCAACAACAAGGGCAACAAGGGCAACACGAGCA
GAAGCAGGAACGTCGACGGCTTCGCGTTCGCTAG

Upstream 100 bases:

>100_bases
CGCTAATAATGCAGTTGATTGGGATAATGCTGCCCAATGTTGGTATGCAAATCCTGGAGCTGATCTTGATAAGTTGAAGC
CGTGGATTGCTACGGAGATC

Downstream 100 bases:

>100_bases
CAGCCAGCCAAGAGGTGGGGGAGCAGCCGACCGGCGAAGAAGTGCGCGAGGTGTATGTATCCGATCAAAATGCAAAAGTG
CATCATTGGATAGATGTATG

Product: TOPRIM domain-containing protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGHPYGYIKNNRTGIELKWKSKG
YSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAID
SDGKQWTMQYIQEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAGNLEGVAKAL
HKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFNDLATKSALGIEAVERQVHAA
VSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR

Sequences:

>Translated_357_residues
MSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGHPYGYIKNNRTGIELKWKSKG
YSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAID
SDGKQWTMQYIQEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAGNLEGVAKAL
HKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFNDLATKSALGIEAVERQVHAA
VSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR
>Mature_356_residues
SRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGHPYGYIKNNRTGIELKWKSKGY
SLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQMDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAIDS
DGKQWTMQYIQEDGTKRFAKGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAGNLEGVAKALH
KSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFNDLATKSALGIEAVERQVHAAV
SKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4643

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 38912; Mature: 38781

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGH
CCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCC
PYGYIKNNRTGIELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQ
CEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAIDSDGKQWTMQYIQEDGTKRFA
HHHHEEEECCCCCEEECCCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
KGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAGNLEGVAKAL
CCCCCCCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
HKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFN
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHH
DLATKSALGIEAVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SRQVPALSPEKEFAETLRSMGCLVSGEHPFMDAKKYRIEVEGDKKGGKAGFYVAYLDGH
CCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCC
PYGYIKNNRTGIELKWKSKGYSLDPEQKAAMQAEATAKLAARAEQQQQEQEAVAQHVVKQ
CEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDDLVTVIEPTPYLKAKGITPQAGIYTDSDGQKTYIPAIDSDGKQWTMQYIQEDGTKRFA
HHHHEEEECCCCCEEECCCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
KGGRKEGCFHPIGGLAAIAAAPVIVIGQGYATVAQVSQALGYGTVAAFDAGNLEGVAKAL
CCCCCCCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
HKSFPDKPILIAGDDDQSLPNNPGRTKAQEAARAVDGTAFFPIFAPGEQADNPKGFTDFN
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHH
DLATKSALGIEAVERQVHAAVSKAIDNHQKRVQKQTQQQGQQGQHEQKQERRRLRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]