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The map label for this gene is acrA [H]

Identifier: 182681380

GI number: 182681380

Start: 976282

End: 977472

Strand: Reverse

Name: acrA [H]

Synonym: XfasM23_0828

Alternate gene names: 182681380

Gene position: 977472-976282 (Counterclockwise)

Preceding gene: 182681385

Following gene: 182681379

Centisome position: 38.55

GC content: 53.48

Gene sequence:

>1191_bases
ATGACTGTCCCGTTACGCGTACTCGGCTTGGTCTACACCGTCTTACTAGCACTTACCGCATGCAGTAAGTCCGAACAGCC
GCAGATGCCAACACCAGAAGTTAGTATCGTCGAAGCAAAGCCGCAAACAACTCCCATACAACTTGATTTAGTCGGACGTC
TATCGGCATACCGCTCCGCAGACGTACGCGCACGGGTGGCTGGAATATTACAAAAGCGGATATACACCGAAGGCACCGAA
GTAAAAGAAGGTCAGCCGCTGTTCCAAATCGACCCTGCGCCATTTCAAGCCACGCTATTAGAAGCGCAAGGCCGTTTAGC
TGCAGCAGAAGCCACGTATAAAAATGCCAAAATCGTCGCTGACCGCGCCCGCCGCTTGTCCCCTGAGCAATACATTTCGC
GCTCGGATGTAGACAATGCCGAGGCAGCAGAACGCACGGCAGCCGCCAGTGTCGAACAAGCACGCGCCGCAATGCAGAGC
GCACGCATCAACCTCAATTACGCCAACGTCACTGCCCCAATCAGCGGTCTTGCTGGCCAACAGCGAGTCACTGAAGGAGC
TTTAGTTGGATCTGGGAGCAGCACCCTACTCACAACCGTTGATCAAATTGATCCGTTGTACGTGAACTTCTCAATGAGCA
ATGATGAGTTGATGCAACTACGCCAAGCACAGCACCAGGGAAGTGTGCAATTGAGTAGCGACAATACATCGACCATCGAC
GTACTCCTCAGTGATGGCAGCAAATATCCACACCAAGGGACATTGGATTTCTCCGGAGCAACTGTTGATCCGAGCACCGG
CACAGTCTCACTGCGTGCAGTGCTTCCAAATACTGATCATCTCCTGATGCCAGGAGCCTTTGTCACATTTAAAGCCAACC
TCGGCCAGCGCAACAATACGATCCTGCTACCCGGCGCAGGGGTGCAACGTGATGCGAACAGCGCTTACGTCCTGGTGATA
GACAAAGATGGCAAGGTCATCCGCAAGAACGTGACCACGACAGGGATGAAAGATAACCAGTGGTTGATTACTGCTGGACT
CTCCAGCGGAGACCGTGTGATCGTTGATGGTCTCCAGAAAGTCAAAGAAGGCGCTCCGGCCAAGGGCATCCCCTGGAAAC
CAGATCCCATTTCCAGCAGCGCACACGCAGCAGCCAAGACACCCACTCACACCGCTAAGACCGAACAATAA

Upstream 100 bases:

>100_bases
AGAATTTTTCAGAAGCATGCAATACATAAATAAAACGTGTCTGCAACAACCAGGGCATATTTCTGTGTCAGATCAACTCA
CAACCTCGGAGCCATTCCCG

Downstream 100 bases:

>100_bases
CGGACGCATCCACCATGCCAAAATTTTTCATTGAGCACCCAATCTTTGCCTGGGTGGTGGCGATCCTGATCTCACTTGCG
GGTGTGATCTCGATCTTCAA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSADVRARVAGILQKRIYTEGTE
VKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQS
ARINLNYANVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGSVQLSSDNTSTID
VLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNTILLPGAGVQRDANSAYVLVI
DKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ

Sequences:

>Translated_396_residues
MTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSADVRARVAGILQKRIYTEGTE
VKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQS
ARINLNYANVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGSVQLSSDNTSTID
VLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNTILLPGAGVQRDANSAYVLVI
DKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ
>Mature_395_residues
TVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSADVRARVAGILQKRIYTEGTEV
KEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVADRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQSA
RINLNYANVTAPISGLAGQQRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGSVQLSSDNTSTIDV
LLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNTILLPGAGVQRDANSAYVLVID
KDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQKVKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=42.3913043478261, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1789929, Length=373, Percent_Identity=39.142091152815, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1789665, Length=360, Percent_Identity=41.6666666666667, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI87082036, Length=339, Percent_Identity=31.2684365781711, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI87081796, Length=323, Percent_Identity=24.1486068111455, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42240; Mature: 42109

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCC
DVRARVAGILQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEHHHHCCCCEEE
DRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQSARINLNYANVTAPISGLAGQ
HHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCC
QRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGSVQLSSDNTSTID
HHHCCCEEECCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCEEE
VLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNT
EEEECCCCCCCCCEEECCCCEECCCCCEEEEEEECCCCCCEEECCEEEEEEECCCCCCCE
ILLPGAGVQRDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQK
EEEECCCCCCCCCCEEEEEECCCCCEEEECCEECCCCCCCEEEEECCCCCCEEHHHHHHH
VKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVPLRVLGLVYTVLLALTACSKSEQPQMPTPEVSIVEAKPQTTPIQLDLVGRLSAYRSA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCC
DVRARVAGILQKRIYTEGTEVKEGQPLFQIDPAPFQATLLEAQGRLAAAEATYKNAKIVA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEHHHHCCCCEEE
DRARRLSPEQYISRSDVDNAEAAERTAAASVEQARAAMQSARINLNYANVTAPISGLAGQ
HHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCC
QRVTEGALVGSGSSTLLTTVDQIDPLYVNFSMSNDELMQLRQAQHQGSVQLSSDNTSTID
HHHCCCEEECCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCEEE
VLLSDGSKYPHQGTLDFSGATVDPSTGTVSLRAVLPNTDHLLMPGAFVTFKANLGQRNNT
EEEECCCCCCCCCEEECCCCEECCCCCEEEEEEECCCCCCEEECCEEEEEEECCCCCCCE
ILLPGAGVQRDANSAYVLVIDKDGKVIRKNVTTTGMKDNQWLITAGLSSGDRVIVDGLQK
EEEECCCCCCCCCCEEEEEECCCCCEEEECCEECCCCCCCEEEEECCCCCCEEHHHHHHH
VKEGAPAKGIPWKPDPISSSAHAAAKTPTHTAKTEQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]