Click here to switch to the map view.

The map label for this gene is cca

Identifier: 182681188

GI number: 182681188

Start: 747394

End: 748644

Strand: Reverse

Name: cca

Synonym: XfasM23_0632

Alternate gene names: 182681188

Gene position: 748644-747394 (Counterclockwise)

Preceding gene: 182681189

Following gene: 182681185

Centisome position: 29.52

GC content: 54.68

Gene sequence:

>1251_bases
ATGAAGAGCTATCTTGTCGGTGGTGCCGTCCGCGACGCATTACTCGGTCAGCCTGCTGGCGACTGCGACTGGGTCGTCGT
TGGTGCCGACCCAGCACACATGAAGAGTTTGGGTTTTAAGCCAGTAGGCCGTGACTTTCCTGTGTTCCTACACCCCAAGA
CCGGCGAGGAATTCGCACTAGCACGTACCGAGCGCAAAAACGGCCACGGCTACCGCGGCTTTATCGTCAACGCTGACCCA
ACAGTCACGCTTGAACAAGATCTGCAACGCCGTGACTTCACCATTAATGCGATCGCACGTGACCAAACCAATGGCACATT
GATCGACCCCTATGGCGGCGTGAATGATCTTGAGCAACGTGTATTGCGCCATATTAGCCCCGCATTTGCCGAGGACCCGT
TGCGCGTGCTGCGTGCAGCACGCTTTATGGCACGGTTGGCACCTTTGGGCTTCAGCATTGCTCCTGAAACGCTGGCAATG
ATGCGTCAGATGGCTGCAAACGGGGAACTCAACAGCTTGATACCCGAAAGAATATGGAAGGAATTAAGCCGATCACTGAC
CTACACACAACCAGCGGCCTTCCTCCATACACTACGCACAGTGAATGCACTGGAGGTGGTGTTACCCGAATTAAACGCAT
TGTATGGAGTACCACAACACGCAGACTATCACCCGGAAATCGACACCGGCCTCCACCAAGAATTGGTCAGCGATATAGCT
GCAAAGCTGGCACCAGGGGATATGCTGATCGGCTTCGCAGCGCTATGTCACGACCTCGGAAAAGCCCTGACACCACGCGC
AACATGGCCTCATCATCCGATGCACGAACAACGCGGGATGGCACCGACACAACAGCTCAGTGAGCGCCTGAAAGTCCCCC
GGAATTACCAGCAACTCGCATTGATCGCCTGCCGCGAACACCTCAACGTACACCGTCTGTCAAAGTTGCATGACAATACC
GTATACGAACTGCTGCAACGCTGTGATGCCTTCCGCCGCCCCGAACGGATCGCACAGCTTGCGATCGTTTGCGAAGCCGA
TTATCGAGGGCGCTATGGACATGAAGATGCTAACTACCCACAAGGACAACATTTATGCCGACTGCACGCCGCAGCGTTGG
CAATCAACGCACGTGACCTTAATAGACAAGATTTACACGGTACACAAATCGGCGAAGCGCTGGCTCAAGCACGCATCCGT
GCGATCAGCTCAGCTGGTGTCTATGATGGGGGAACGGGAACAAATTTCTGA

Upstream 100 bases:

>100_bases
GCCAGTGAGCACGACGCATCCCATAACAACCCAGCCCAGGCCACTTGGCGGCCACTTTTGGGCAGTGTCCCCCCTTCGCA
CCTCATCGCTGAGATGATGC

Downstream 100 bases:

>100_bases
CGTTTGCTACGAAGCATAAAATCAAAACGGCATATTTCAGTTGATTGGCCACATATCGAAGAAACCGGACACAACCACCG
ATATCTGCCTCGCCGTACCA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKSYLVGGAVRDALLGQPAGDCDWVVVGADPAHMKSLGFKPVGRDFPVFLHPKTGEEFALARTERKNGHGYRGFIVNADP
TVTLEQDLQRRDFTINAIARDQTNGTLIDPYGGVNDLEQRVLRHISPAFAEDPLRVLRAARFMARLAPLGFSIAPETLAM
MRQMAANGELNSLIPERIWKELSRSLTYTQPAAFLHTLRTVNALEVVLPELNALYGVPQHADYHPEIDTGLHQELVSDIA
AKLAPGDMLIGFAALCHDLGKALTPRATWPHHPMHEQRGMAPTQQLSERLKVPRNYQQLALIACREHLNVHRLSKLHDNT
VYELLQRCDAFRRPERIAQLAIVCEADYRGRYGHEDANYPQGQHLCRLHAAALAINARDLNRQDLHGTQIGEALAQARIR
AISSAGVYDGGTGTNF

Sequences:

>Translated_416_residues
MKSYLVGGAVRDALLGQPAGDCDWVVVGADPAHMKSLGFKPVGRDFPVFLHPKTGEEFALARTERKNGHGYRGFIVNADP
TVTLEQDLQRRDFTINAIARDQTNGTLIDPYGGVNDLEQRVLRHISPAFAEDPLRVLRAARFMARLAPLGFSIAPETLAM
MRQMAANGELNSLIPERIWKELSRSLTYTQPAAFLHTLRTVNALEVVLPELNALYGVPQHADYHPEIDTGLHQELVSDIA
AKLAPGDMLIGFAALCHDLGKALTPRATWPHHPMHEQRGMAPTQQLSERLKVPRNYQQLALIACREHLNVHRLSKLHDNT
VYELLQRCDAFRRPERIAQLAIVCEADYRGRYGHEDANYPQGQHLCRLHAAALAINARDLNRQDLHGTQIGEALAQARIR
AISSAGVYDGGTGTNF
>Mature_416_residues
MKSYLVGGAVRDALLGQPAGDCDWVVVGADPAHMKSLGFKPVGRDFPVFLHPKTGEEFALARTERKNGHGYRGFIVNADP
TVTLEQDLQRRDFTINAIARDQTNGTLIDPYGGVNDLEQRVLRHISPAFAEDPLRVLRAARFMARLAPLGFSIAPETLAM
MRQMAANGELNSLIPERIWKELSRSLTYTQPAAFLHTLRTVNALEVVLPELNALYGVPQHADYHPEIDTGLHQELVSDIA
AKLAPGDMLIGFAALCHDLGKALTPRATWPHHPMHEQRGMAPTQQLSERLKVPRNYQQLALIACREHLNVHRLSKLHDNT
VYELLQRCDAFRRPERIAQLAIVCEADYRGRYGHEDANYPQGQHLCRLHAAALAINARDLNRQDLHGTQIGEALAQARIR
AISSAGVYDGGTGTNF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=52.7227722772277, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI87081691, Length=198, Percent_Identity=35.3535353535354, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_XYLF2 (B2I9I0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829348.1
- ProteinModelPortal:   B2I9I0
- SMR:   B2I9I0
- GeneID:   6203222
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0632
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- ProtClustDB:   PRK10885
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46204; Mature: 46204

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 138-138 BINDING 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYLVGGAVRDALLGQPAGDCDWVVVGADPAHMKSLGFKPVGRDFPVFLHPKTGEEFAL
CCCCCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCCHHHH
ARTERKNGHGYRGFIVNADPTVTLEQDLQRRDFTINAIARDQTNGTLIDPYGGVNDLEQR
HHHHCCCCCCEEEEEECCCCCEEHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHH
VLRHISPAFAEDPLRVLRAARFMARLAPLGFSIAPETLAMMRQMAANGELNSLIPERIWK
HHHHCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ELSRSLTYTQPAAFLHTLRTVNALEVVLPELNALYGVPQHADYHPEIDTGLHQELVSDIA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
AKLAPGDMLIGFAALCHDLGKALTPRATWPHHPMHEQRGMAPTQQLSERLKVPRNYQQLA
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHH
LIACREHLNVHRLSKLHDNTVYELLQRCDAFRRPERIAQLAIVCEADYRGRYGHEDANYP
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCCCCCCC
QGQHLCRLHAAALAINARDLNRQDLHGTQIGEALAQARIRAISSAGVYDGGTGTNF
CHHHHHHHHHHHHHCCHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKSYLVGGAVRDALLGQPAGDCDWVVVGADPAHMKSLGFKPVGRDFPVFLHPKTGEEFAL
CCCCCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCCHHHH
ARTERKNGHGYRGFIVNADPTVTLEQDLQRRDFTINAIARDQTNGTLIDPYGGVNDLEQR
HHHHCCCCCCEEEEEECCCCCEEHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHH
VLRHISPAFAEDPLRVLRAARFMARLAPLGFSIAPETLAMMRQMAANGELNSLIPERIWK
HHHHCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ELSRSLTYTQPAAFLHTLRTVNALEVVLPELNALYGVPQHADYHPEIDTGLHQELVSDIA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
AKLAPGDMLIGFAALCHDLGKALTPRATWPHHPMHEQRGMAPTQQLSERLKVPRNYQQLA
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHH
LIACREHLNVHRLSKLHDNTVYELLQRCDAFRRPERIAQLAIVCEADYRGRYGHEDANYP
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCCCCCCC
QGQHLCRLHAAALAINARDLNRQDLHGTQIGEALAQARIRAISSAGVYDGGTGTNF
CHHHHHHHHHHHHHCCHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA