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The map label for this gene is slt [H]

Identifier: 182681189

GI number: 182681189

Start: 748737

End: 750686

Strand: Reverse

Name: slt [H]

Synonym: XfasM23_0633

Alternate gene names: 182681189

Gene position: 750686-748737 (Counterclockwise)

Preceding gene: 182681190

Following gene: 182681188

Centisome position: 29.6

GC content: 55.23

Gene sequence:

>1950_bases
ATGACTATGCTATTACGTTTAGTGACTGCACTAACGCTGGGCATTGCAGCCTGTGCCCGTGCACAAGGTGACGACCCACA
ACTGATGGCTGTTCGCGATGCCATCGCCGCAGCCGAAAATGGTCGCCTCGACCCATCTCAAGCAGCAACTCTGAACAAAC
ATCCGTTATACGGCTGGATCGAATACGCCAATCTACGCCGCAATATCGACACAATCAGCAATGCGCAGGCGCAAAGCTTC
CTATCACGTTACGCCGACCAAGCCGTCGCTCAGGCATTCCGCTCAGCATGGCTGCCAATCGTCGTGCGGCGTAAAGACTG
GAACACACTGCTAACACATTGGAAGCCGACCCGTACCGTCAGTCTACGTTGTGCCCAACTGGAAGCACGCTATCGTTTAG
GCAAAGCAGACACCCAGTGGACCAAAGAAGCACAAGAACTATGGCGCGATGCCGACAAACCAGTGCCTAGCATCTGTGAT
TCCGTATTCGCCACACTCTCTGCTCAAGGAGGCCTGGATAATGCACTGCGCTGGGCACGCATCGAAGCTGCTGCCGATGC
GCAACAACCCGAAGTCATGCGCGCCGCAGCACGCGGCCTGCCAGCCAGTGATCAAACCCTGGCTAACAACTACGCCGCAT
TTGTCGAAGCGCCAAATCAAGCCCAGGCCGTATCGAATTGGCCCAAGACGGAACGTAGCCAACGCATCGTTATTGACGGA
TTAGCACGCATGGCCAAAGCTGATCCGAGCGCCGCTGAACGTTCCTTACCCAAGTACACCGATGCCCTTAACTTCAATGA
GGCGCAGCGTGGTCAAGTGCTGTACCAAATCGCATTATGGACCGTCGCCTCCTACCAACCAGAATCAGCACGGCGACTGG
CAGCAGTACCAGAATCGGCCTACGACGAACGTTTGCACGAATGGCGTACCCGCGAAGCAATGGCACGTGGCGACTGGCAA
GCTGCATTAAATGCGATCCGCGAAATGCCACCGACACAGCGTGGCGAGCCCCGCTGGCAATACTTCGAAGCACGCTTGGC
CGAAAAGACCGGCGACAGCGTACAGAGCCAAACGCTGTACCGTGCTGCTGCACGCAGTGCGACATTTCATGGTTTCCTTG
CAGCAGACCATCTGCAACAACCCTATTCACTCTGCCCGTGGCATCCCAACGATACCCCTGACCACGCCCAGGCCAAAGCC
GCTGTTGCACGCACTCCAGCACTGGTACGTGCGATAGCACTGTTCCAGATTGACCGGACAGCTTGGGCCACACTTGAATG
GAACAACGCACTCACACGCTTTAATGACAACGAACGCCGACTCGCAGTAGAAGTGGCCAGTGACGCAGAATGGTTCGACC
GTGCCGTATTCGCACTTGGTAAGCAGCCTGCCGAGCTACGCATGTACGACCTGCGCTTCCCATTACGCCATGAAACTACG
ATCCGCAGTGAAGCAACCAAAAACGGATTGGACCCAGCCTGGATCACTGCCGAAATCCGAGCAGAAAGCGTCTTCAACCC
ACGCGCCCGCTCCCCAGCAAATGCACGTGGTCTGATGCAAGTGCTACCCAGCACAGCAGCAAGCGTCGCCAAACGCATTG
GTCTCACAGACTATGTCAACGCCGATAGCCTATACGATGCAGACACAAACATCATTATTGGCAGTGCTTACCTACGCCAA
CTCCTTGACCAATACAGCCAGACGTATCTGACCATTGCAGCTTACAACGGAGGCCCAGGCTCAGTGCAACGCTGGTTATC
ACAGCGCCCCAACTATGATCCGGATCTCTGGATCGAAACCATCAACTACAAAGAAACACGCGAATATGTCGCGCGTGTGC
TTGCTTTTAGCGTGATCTATGACTGGCGCATCAACGGCGATACACTGCTCCTCACTGATCGTTTACTTGGCAAGTCCACT
CACCGACGTAAACAAATCATCTGCCAGTGA

Upstream 100 bases:

>100_bases
ATTGCAAACACAACTATCACGTGAAACAAATCACCTACTCCCACACAAGCCCCGCATAGTGAACAAATATTCAGGCGGCG
ACTTTGATATATTCCCTGGA

Downstream 100 bases:

>100_bases
GCACGACGCATCCCATAACAACCCAGCCCAGGCCACTTGGCGGCCACTTTTGGGCAGTGTCCCCCCTTCGCACCTCATCG
CTGAGATGATGCATGAAGAG

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MTMLLRLVTALTLGIAACARAQGDDPQLMAVRDAIAAAENGRLDPSQAATLNKHPLYGWIEYANLRRNIDTISNAQAQSF
LSRYADQAVAQAFRSAWLPIVVRRKDWNTLLTHWKPTRTVSLRCAQLEARYRLGKADTQWTKEAQELWRDADKPVPSICD
SVFATLSAQGGLDNALRWARIEAAADAQQPEVMRAAARGLPASDQTLANNYAAFVEAPNQAQAVSNWPKTERSQRIVIDG
LARMAKADPSAAERSLPKYTDALNFNEAQRGQVLYQIALWTVASYQPESARRLAAVPESAYDERLHEWRTREAMARGDWQ
AALNAIREMPPTQRGEPRWQYFEARLAEKTGDSVQSQTLYRAAARSATFHGFLAADHLQQPYSLCPWHPNDTPDHAQAKA
AVARTPALVRAIALFQIDRTAWATLEWNNALTRFNDNERRLAVEVASDAEWFDRAVFALGKQPAELRMYDLRFPLRHETT
IRSEATKNGLDPAWITAEIRAESVFNPRARSPANARGLMQVLPSTAASVAKRIGLTDYVNADSLYDADTNIIIGSAYLRQ
LLDQYSQTYLTIAAYNGGPGSVQRWLSQRPNYDPDLWIETINYKETREYVARVLAFSVIYDWRINGDTLLLTDRLLGKST
HRRKQIICQ

Sequences:

>Translated_649_residues
MTMLLRLVTALTLGIAACARAQGDDPQLMAVRDAIAAAENGRLDPSQAATLNKHPLYGWIEYANLRRNIDTISNAQAQSF
LSRYADQAVAQAFRSAWLPIVVRRKDWNTLLTHWKPTRTVSLRCAQLEARYRLGKADTQWTKEAQELWRDADKPVPSICD
SVFATLSAQGGLDNALRWARIEAAADAQQPEVMRAAARGLPASDQTLANNYAAFVEAPNQAQAVSNWPKTERSQRIVIDG
LARMAKADPSAAERSLPKYTDALNFNEAQRGQVLYQIALWTVASYQPESARRLAAVPESAYDERLHEWRTREAMARGDWQ
AALNAIREMPPTQRGEPRWQYFEARLAEKTGDSVQSQTLYRAAARSATFHGFLAADHLQQPYSLCPWHPNDTPDHAQAKA
AVARTPALVRAIALFQIDRTAWATLEWNNALTRFNDNERRLAVEVASDAEWFDRAVFALGKQPAELRMYDLRFPLRHETT
IRSEATKNGLDPAWITAEIRAESVFNPRARSPANARGLMQVLPSTAASVAKRIGLTDYVNADSLYDADTNIIIGSAYLRQ
LLDQYSQTYLTIAAYNGGPGSVQRWLSQRPNYDPDLWIETINYKETREYVARVLAFSVIYDWRINGDTLLLTDRLLGKST
HRRKQIICQ
>Mature_648_residues
TMLLRLVTALTLGIAACARAQGDDPQLMAVRDAIAAAENGRLDPSQAATLNKHPLYGWIEYANLRRNIDTISNAQAQSFL
SRYADQAVAQAFRSAWLPIVVRRKDWNTLLTHWKPTRTVSLRCAQLEARYRLGKADTQWTKEAQELWRDADKPVPSICDS
VFATLSAQGGLDNALRWARIEAAADAQQPEVMRAAARGLPASDQTLANNYAAFVEAPNQAQAVSNWPKTERSQRIVIDGL
ARMAKADPSAAERSLPKYTDALNFNEAQRGQVLYQIALWTVASYQPESARRLAAVPESAYDERLHEWRTREAMARGDWQA
ALNAIREMPPTQRGEPRWQYFEARLAEKTGDSVQSQTLYRAAARSATFHGFLAADHLQQPYSLCPWHPNDTPDHAQAKAA
VARTPALVRAIALFQIDRTAWATLEWNNALTRFNDNERRLAVEVASDAEWFDRAVFALGKQPAELRMYDLRFPLRHETTI
RSEATKNGLDPAWITAEIRAESVFNPRARSPANARGLMQVLPSTAASVAKRIGLTDYVNADSLYDADTNIIIGSAYLRQL
LDQYSQTYLTIAAYNGGPGSVQRWLSQRPNYDPDLWIETINYKETREYVARVLAFSVIYDWRINGDTLLLTDRLLGKSTH
RRKQIICQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=631, Percent_Identity=25.6735340729002, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082191, Length=117, Percent_Identity=39.3162393162393, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73015; Mature: 72884

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLLRLVTALTLGIAACARAQGDDPQLMAVRDAIAAAENGRLDPSQAATLNKHPLYGWI
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHH
EYANLRRNIDTISNAQAQSFLSRYADQAVAQAFRSAWLPIVVRRKDWNTLLTHWKPTRTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEE
SLRCAQLEARYRLGKADTQWTKEAQELWRDADKPVPSICDSVFATLSAQGGLDNALRWAR
EHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
IEAAADAQQPEVMRAAARGLPASDQTLANNYAAFVEAPNQAQAVSNWPKTERSQRIVIDG
HHHHCCCCCHHHHHHHHCCCCCCCHHHHCCCEEEEECCCHHHHHHCCCCCCCCCEEHHHH
LARMAKADPSAAERSLPKYTDALNFNEAQRGQVLYQIALWTVASYQPESARRLAAVPESA
HHHHHCCCCCHHHHCCCHHHHCCCCCCHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
YDERLHEWRTREAMARGDWQAALNAIREMPPTQRGEPRWQYFEARLAEKTGDSVQSQTLY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
RAAARSATFHGFLAADHLQQPYSLCPWHPNDTPDHAQAKAAVARTPALVRAIALFQIDRT
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AWATLEWNNALTRFNDNERRLAVEVASDAEWFDRAVFALGKQPAELRMYDLRFPLRHETT
EEEEEEECCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHEEEEEECCCCCCCHH
IRSEATKNGLDPAWITAEIRAESVFNPRARSPANARGLMQVLPSTAASVAKRIGLTDYVN
HHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
ADSLYDADTNIIIGSAYLRQLLDQYSQTYLTIAAYNGGPGSVQRWLSQRPNYDPDLWIET
CCCCCCCCCCEEECHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEE
INYKETREYVARVLAFSVIYDWRINGDTLLLTDRLLGKSTHRRKQIICQ
CCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEHHHHCCCHHHHHHCCCC
>Mature Secondary Structure 
TMLLRLVTALTLGIAACARAQGDDPQLMAVRDAIAAAENGRLDPSQAATLNKHPLYGWI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHH
EYANLRRNIDTISNAQAQSFLSRYADQAVAQAFRSAWLPIVVRRKDWNTLLTHWKPTRTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEE
SLRCAQLEARYRLGKADTQWTKEAQELWRDADKPVPSICDSVFATLSAQGGLDNALRWAR
EHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
IEAAADAQQPEVMRAAARGLPASDQTLANNYAAFVEAPNQAQAVSNWPKTERSQRIVIDG
HHHHCCCCCHHHHHHHHCCCCCCCHHHHCCCEEEEECCCHHHHHHCCCCCCCCCEEHHHH
LARMAKADPSAAERSLPKYTDALNFNEAQRGQVLYQIALWTVASYQPESARRLAAVPESA
HHHHHCCCCCHHHHCCCHHHHCCCCCCHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
YDERLHEWRTREAMARGDWQAALNAIREMPPTQRGEPRWQYFEARLAEKTGDSVQSQTLY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
RAAARSATFHGFLAADHLQQPYSLCPWHPNDTPDHAQAKAAVARTPALVRAIALFQIDRT
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AWATLEWNNALTRFNDNERRLAVEVASDAEWFDRAVFALGKQPAELRMYDLRFPLRHETT
EEEEEEECCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHEEEEEECCCCCCCHH
IRSEATKNGLDPAWITAEIRAESVFNPRARSPANARGLMQVLPSTAASVAKRIGLTDYVN
HHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
ADSLYDADTNIIIGSAYLRQLLDQYSQTYLTIAAYNGGPGSVQRWLSQRPNYDPDLWIET
CCCCCCCCCCEEECHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEE
INYKETREYVARVLAFSVIYDWRINGDTLLLTDRLLGKSTHRRKQIICQ
CCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]