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The map label for this gene is ispF

Identifier: 182681129

GI number: 182681129

Start: 680111

End: 680614

Strand: Direct

Name: ispF

Synonym: XfasM23_0571

Alternate gene names: 182681129

Gene position: 680111-680614 (Clockwise)

Preceding gene: 182681128

Following gene: 182681133

Centisome position: 26.82

GC content: 54.37

Gene sequence:

>504_bases
ATGACGGGTTTGAATGTTCGCATCGGTCAGGGGTATGACGTGCATGCGTTTGGACCCAGTGATCATGTGATGTTGGGTGG
GGTGCGAGTGCCGCATCGTTGCGGTGTGTTGGCACACAGTGATGGTGATGTGATCTTGCATGCGTTGTGTGATGCCATGC
TTGGTGCGTTGGGGTTGGGTGACATTGGTCAGCACTTTCCACCTTCCGATGTACGTTGGAAGGGAGCAGACAGTGGTGTC
TTTGTGCGTCACTGCAACCTGTTGCTGCGCGAACGTGATTGGTGTGTCGGTAATGTTGATGCAACTGCAATCTGCGAATC
GCCGAAGATTGCTCCGTATGTCAATGCGATGCGTGAGGGGATTGCGCGCTTGCTTGAAGTGCAGCCGGAGTGTGTCAGTG
TAAAGGCTACGACGAGCGAGGGACTTGGTTTTATTGGTCGCGGTGAGGGCCTTGCTGCTCAGGTCGTGGTATTGCTGTAT
CGCCTTAACGGTGTCGTGGTGTAG

Upstream 100 bases:

>100_bases
GTGCCGAGAGTAATTTTAAGATCACGACGCCAGATGATTTGGTGCGTTTCGAGTTTGAATTGGCGCGGCGTGTTTGAGTG
GCTTATTTAGGGGGAGGGTG

Downstream 100 bases:

>100_bases
GGGACAAAGAATATTGCTTGATCGCTATAGAGTTAAGTCTTGGGTGTATCCACTCCGATCTTCCGTTGCGATGTGGGTGG
TTGATGAGTTAGGGGTTGTG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTGLNVRIGQGYDVHAFGPSDHVMLGGVRVPHRCGVLAHSDGDVILHALCDAMLGALGLGDIGQHFPPSDVRWKGADSGV
FVRHCNLLLRERDWCVGNVDATAICESPKIAPYVNAMREGIARLLEVQPECVSVKATTSEGLGFIGRGEGLAAQVVVLLY
RLNGVVV

Sequences:

>Translated_167_residues
MTGLNVRIGQGYDVHAFGPSDHVMLGGVRVPHRCGVLAHSDGDVILHALCDAMLGALGLGDIGQHFPPSDVRWKGADSGV
FVRHCNLLLRERDWCVGNVDATAICESPKIAPYVNAMREGIARLLEVQPECVSVKATTSEGLGFIGRGEGLAAQVVVLLY
RLNGVVV
>Mature_166_residues
TGLNVRIGQGYDVHAFGPSDHVMLGGVRVPHRCGVLAHSDGDVILHALCDAMLGALGLGDIGQHFPPSDVRWKGADSGVF
VRHCNLLLRERDWCVGNVDATAICESPKIAPYVNAMREGIARLLEVQPECVSVKATTSEGLGFIGRGEGLAAQVVVLLYR
LNGVVV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=51.2820512820513, Blast_Score=148, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17767; Mature: 17636

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLNVRIGQGYDVHAFGPSDHVMLGGVRVPHRCGVLAHSDGDVILHALCDAMLGALGLG
CCCCEEEECCCEEEEEECCCCCEEECCEECCHHCCEEEECCCCHHHHHHHHHHHHHCCCC
DIGQHFPPSDVRWKGADSGVFVRHCNLLLRERDWCVGNVDATAICESPKIAPYVNAMREG
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCEECCCCCEEEECCCCCCHHHHHHHHH
IARLLEVQPECVSVKATTSEGLGFIGRGEGLAAQVVVLLYRLNGVVV
HHHHHHCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TGLNVRIGQGYDVHAFGPSDHVMLGGVRVPHRCGVLAHSDGDVILHALCDAMLGALGLG
CCCEEEECCCEEEEEECCCCCEEECCEECCHHCCEEEECCCCHHHHHHHHHHHHHCCCC
DIGQHFPPSDVRWKGADSGVFVRHCNLLLRERDWCVGNVDATAICESPKIAPYVNAMREG
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCEECCCCCEEEECCCCCCHHHHHHHHH
IARLLEVQPECVSVKATTSEGLGFIGRGEGLAAQVVVLLYRLNGVVV
HHHHHHCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]