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The map label for this gene is ispD

Identifier: 182681128

GI number: 182681128

Start: 679392

End: 680087

Strand: Direct

Name: ispD

Synonym: XfasM23_0570

Alternate gene names: 182681128

Gene position: 679392-680087 (Clockwise)

Preceding gene: 182681127

Following gene: 182681129

Centisome position: 26.79

GC content: 57.9

Gene sequence:

>696_bases
ATGAGTGTTGGTGTTTGGGCGGTGATCCCTGCGGCTGGCCGTGGGGTCCGATTTGGTTCTCCTGTGCCCAAACAGTATTT
ACCCGTTGTTGGGCGGCCGTTGATTGTCTATACCCTGGAAGCATTGGCAGCACATCCGGCGGTGTGTGGGTTGATGGTGG
TGGTGGCTGAGGGCGATCTGGCTTGGTCCGGCTGGACAGAGCTGGCAGGTAAGCCATTGTTGACTTGCAGTGGAGGCGTG
ACCCGTGCTGCTTCGGTATTGTCTGGCTTGTTGGCGTTGCCACAGGTGGTGCACGCTGATGATTTCGTGTTGGTCCATGA
TGCTGCGCGTCCCAACGTGGCTTTGTCCGATCTGGAACGATTGCTGGAGGCGGGCTGTGCCCATCCAGTGGGTGCGATCC
TGGCTGTACCGGTGCGCGACACGCTGAAGCGTGCCGGTTCTGATGGCAGTATTGACGGCACCGAGCCACGTGAACGGTTA
TGGCGTGCATTCACTCCACAGTTGTTTCGGCGATCTCAGTTGGTGCGCGGTTTGCAGGTCGCTGCAGCGGATGGGATCGA
GATGACCGATGAAGCGATGGTCATGGAGCGGCAGGGATTGCGTCCTCTTCTGGTGGAGTGTGCCGAGAGTAATTTTAAGA
TCACGACGCCAGATGATTTGGTGCGTTTCGAGTTTGAATTGGCGCGGCGTGTTTGA

Upstream 100 bases:

>100_bases
AGATCTTCTATCGTGTGGTTGAGGATATTCCTGTGCCTCTCCCCAACGATACATCTGCAGATCATGGAGTTGATCTGTCG
CAACCTCGTAGAGAAAAACG

Downstream 100 bases:

>100_bases
GTGGCTTATTTAGGGGGAGGGTGATGACGGGTTTGAATGTTCGCATCGGTCAGGGGTATGACGTGCATGCGTTTGGACCC
AGTGATCATGTGATGTTGGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSVGVWAVIPAAGRGVRFGSPVPKQYLPVVGRPLIVYTLEALAAHPAVCGLMVVVAEGDLAWSGWTELAGKPLLTCSGGV
TRAASVLSGLLALPQVVHADDFVLVHDAARPNVALSDLERLLEAGCAHPVGAILAVPVRDTLKRAGSDGSIDGTEPRERL
WRAFTPQLFRRSQLVRGLQVAAADGIEMTDEAMVMERQGLRPLLVECAESNFKITTPDDLVRFEFELARRV

Sequences:

>Translated_231_residues
MSVGVWAVIPAAGRGVRFGSPVPKQYLPVVGRPLIVYTLEALAAHPAVCGLMVVVAEGDLAWSGWTELAGKPLLTCSGGV
TRAASVLSGLLALPQVVHADDFVLVHDAARPNVALSDLERLLEAGCAHPVGAILAVPVRDTLKRAGSDGSIDGTEPRERL
WRAFTPQLFRRSQLVRGLQVAAADGIEMTDEAMVMERQGLRPLLVECAESNFKITTPDDLVRFEFELARRV
>Mature_230_residues
SVGVWAVIPAAGRGVRFGSPVPKQYLPVVGRPLIVYTLEALAAHPAVCGLMVVVAEGDLAWSGWTELAGKPLLTCSGGVT
RAASVLSGLLALPQVVHADDFVLVHDAARPNVALSDLERLLEAGCAHPVGAILAVPVRDTLKRAGSDGSIDGTEPRERLW
RAFTPQLFRRSQLVRGLQVAAADGIEMTDEAMVMERQGLRPLLVECAESNFKITTPDDLVRFEFELARRV

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=241, Percent_Identity=30.7053941908714, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI157671913, Length=135, Percent_Identity=36.2962962962963, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789104, Length=231, Percent_Identity=41.991341991342, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_XYLF2 (B2I939)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829288.1
- ProteinModelPortal:   B2I939
- SMR:   B2I939
- GeneID:   6202693
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0570
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24767; Mature: 24636

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGVWAVIPAAGRGVRFGSPVPKQYLPVVGRPLIVYTLEALAAHPAVCGLMVVVAEGDL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCHHHHHEEEEEECCCC
AWSGWTELAGKPLLTCSGGVTRAASVLSGLLALPQVVHADDFVLVHDAARPNVALSDLER
CCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
LLEAGCAHPVGAILAVPVRDTLKRAGSDGSIDGTEPRERLWRAFTPQLFRRSQLVRGLQV
HHHHCCCCCCHHEEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
AAADGIEMTDEAMVMERQGLRPLLVECAESNFKITTPDDLVRFEFELARRV
HHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVGVWAVIPAAGRGVRFGSPVPKQYLPVVGRPLIVYTLEALAAHPAVCGLMVVVAEGDL
CCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCHHHHHEEEEEECCCC
AWSGWTELAGKPLLTCSGGVTRAASVLSGLLALPQVVHADDFVLVHDAARPNVALSDLER
CCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
LLEAGCAHPVGAILAVPVRDTLKRAGSDGSIDGTEPRERLWRAFTPQLFRRSQLVRGLQV
HHHHCCCCCCHHEEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
AAADGIEMTDEAMVMERQGLRPLLVECAESNFKITTPDDLVRFEFELARRV
HHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA