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The map label for this gene is kdsA

Identifier: 182681125

GI number: 182681125

Start: 676462

End: 677292

Strand: Direct

Name: kdsA

Synonym: XfasM23_0567

Alternate gene names: 182681125

Gene position: 676462-677292 (Clockwise)

Preceding gene: 182681124

Following gene: 182681126

Centisome position: 26.68

GC content: 52.11

Gene sequence:

>831_bases
ATGAAGCTTTGTGGTTTTGAAGTCGGTCTTAACCAGCCACTTTTCCTGATTGCCGGTCCTTGTGTGATTGAGTCGCTACA
GTTGCAGTTGGATACTGCCGGTGTACTGAAGGAGATCACTTCTAAGCTTGGTTTGAACTTTATCTTTAAGTCCAGTTTTG
ATAAGGCGAACCGAACTTCAGGGAGTAGTTTTCGTGGCCCTGGCTTGGAAGAGGGGTTGAAGGTACTGGAGGCGGTGAAG
ACGCAGATTGGGGTGCCAGTACTGACGGATGTGCACGAGTACACGCCAATAGACGAGGTAGCCACTGTTGTTGATGTGCT
GCAAACGCCAGCGTTTTTGGTACGTCAGACTGATTTCATACGGAATGTCTGTGCGGTCGGTAAACCGGTCAACATCAAGA
AGGGACAGTTTTTGTCGCCATGGGATATGAAACCTGTGGTTGAGAAAGCAAAGTCGACGGGTAATTCGCAGATTCTTGTA
TGTGAACGCGGCGCTTCGTTCGGTTACAACAACTTGGTTAGCGATATGCGTTCACTGGCGGTGATGCGCGAGACCGGCTG
TCCGGTGGTATTCGACGCGACTCATTCTGTGCAACTTCCGGGTGCTCAGGGTGGTCGTTCCGGTGGGCAACGCGAGTTCG
TACCGGTGTTGGCGAGGGCAGCTGTGGCGGTGGGTATTTCCGGATTGTTCGCTGAGACTCACCCAGACCCACCCAATGCA
TTGTCTGATGGCCCCAATGCTTGGCCGTTGCGCACAATGGCGATGTTGTTGGAAACCTTGGTGGAGTTGGATGCGGTCAC
CAAAAAACGAGGTTTCCTCGAGCAGGATTGA

Upstream 100 bases:

>100_bases
TCAAGATATCTTCAGGGAGGTTGAGGGAGGGACATGTCCTGCGTTGGGTTTGTCGTTTTTTTCTGTGTTGCTCGTGATTC
CTAAACAATCAGGTGATGTG

Downstream 100 bases:

>100_bases
ATCAATCTGCCGGTTGCAGGAAGGCTTTGGTTTGGATGGATGGTATTCCTTAGTTGCCTTGCTTATTTTTGTGTGTTGGG
CTGGTGATCGTCCTCCGTGT

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKLCGFEVGLNQPLFLIAGPCVIESLQLQLDTAGVLKEITSKLGLNFIFKSSFDKANRTSGSSFRGPGLEEGLKVLEAVK
TQIGVPVLTDVHEYTPIDEVATVVDVLQTPAFLVRQTDFIRNVCAVGKPVNIKKGQFLSPWDMKPVVEKAKSTGNSQILV
CERGASFGYNNLVSDMRSLAVMRETGCPVVFDATHSVQLPGAQGGRSGGQREFVPVLARAAVAVGISGLFAETHPDPPNA
LSDGPNAWPLRTMAMLLETLVELDAVTKKRGFLEQD

Sequences:

>Translated_276_residues
MKLCGFEVGLNQPLFLIAGPCVIESLQLQLDTAGVLKEITSKLGLNFIFKSSFDKANRTSGSSFRGPGLEEGLKVLEAVK
TQIGVPVLTDVHEYTPIDEVATVVDVLQTPAFLVRQTDFIRNVCAVGKPVNIKKGQFLSPWDMKPVVEKAKSTGNSQILV
CERGASFGYNNLVSDMRSLAVMRETGCPVVFDATHSVQLPGAQGGRSGGQREFVPVLARAAVAVGISGLFAETHPDPPNA
LSDGPNAWPLRTMAMLLETLVELDAVTKKRGFLEQD
>Mature_276_residues
MKLCGFEVGLNQPLFLIAGPCVIESLQLQLDTAGVLKEITSKLGLNFIFKSSFDKANRTSGSSFRGPGLEEGLKVLEAVK
TQIGVPVLTDVHEYTPIDEVATVVDVLQTPAFLVRQTDFIRNVCAVGKPVNIKKGQFLSPWDMKPVVEKAKSTGNSQILV
CERGASFGYNNLVSDMRSLAVMRETGCPVVFDATHSVQLPGAQGGRSGGQREFVPVLARAAVAVGISGLFAETHPDPPNA
LSDGPNAWPLRTMAMLLETLVELDAVTKKRGFLEQD

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=266, Percent_Identity=46.2406015037594, Blast_Score=244, Evalue=4e-66,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_XYLF2 (B2I936)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829285.1
- ProteinModelPortal:   B2I936
- SMR:   B2I936
- GeneID:   6202903
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0567
- HOGENOM:   HBG575990
- OMA:   GVFLECH
- ProtClustDB:   PRK05198
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29698; Mature: 29698

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCGFEVGLNQPLFLIAGPCVIESLQLQLDTAGVLKEITSKLGLNFIFKSSFDKANRTS
CCCCCEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
GSSFRGPGLEEGLKVLEAVKTQIGVPVLTDVHEYTPIDEVATVVDVLQTPAFLVRQTDFI
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHH
RNVCAVGKPVNIKKGQFLSPWDMKPVVEKAKSTGNSQILVCERGASFGYNNLVSDMRSLA
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHH
VMRETGCPVVFDATHSVQLPGAQGGRSGGQREFVPVLARAAVAVGISGLFAETHPDPPNA
HHHHCCCCEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LSDGPNAWPLRTMAMLLETLVELDAVTKKRGFLEQD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLCGFEVGLNQPLFLIAGPCVIESLQLQLDTAGVLKEITSKLGLNFIFKSSFDKANRTS
CCCCCEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
GSSFRGPGLEEGLKVLEAVKTQIGVPVLTDVHEYTPIDEVATVVDVLQTPAFLVRQTDFI
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHH
RNVCAVGKPVNIKKGQFLSPWDMKPVVEKAKSTGNSQILVCERGASFGYNNLVSDMRSLA
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHH
VMRETGCPVVFDATHSVQLPGAQGGRSGGQREFVPVLARAAVAVGISGLFAETHPDPPNA
HHHHCCCCEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LSDGPNAWPLRTMAMLLETLVELDAVTKKRGFLEQD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA