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The map label for this gene is eno

Identifier: 182681126

GI number: 182681126

Start: 677743

End: 679035

Strand: Direct

Name: eno

Synonym: XfasM23_0568

Alternate gene names: 182681126

Gene position: 677743-679035 (Clockwise)

Preceding gene: 182681125

Following gene: 182681127

Centisome position: 26.73

GC content: 50.89

Gene sequence:

>1293_bases
ATGACCGTTATTGCCAAAATTTATGCTCGTGAGATTCTTGATTCCCGTGGCAACCCTACTCTTGAAGCGGAGGTCACTCT
GGAGAATGCTGTCTGTGGCCGTGCCGCAGTCCCCTCAGGTGCTTCGACTGGTACCAAGGAAGCGGTGGAATTACGTGATG
GTGACAAGACACGTTACTTAGGCAAGGGGGTGCGTGCAGCGGTGGATAACGTCAATGGTGTTATTGCTGCGGCGTTGGTG
GGTTTCGATGGAGCTGACCAAACAGGCTTGGACCATCGTTTAATCAATCTGGACGGCACCGAGAATAAGGGGCGTTTGGG
CGCTAACGCGCTGCTCGGTGTTTCGTTAGCAACCGCACATGCGGTTGCCGCCGCGCGTAAACAGCCGCTATGGATGTATT
TGTCCACCCTTGGTGAATCCAAGTTATCGTTGCCGGTGCCAATGATGAACATCATCAATGGTGGTGCGCATGCGGATAAC
AACGTTGATTTCCAGGAGTTTATGGTGTTGCCCGTTGGTTTTGCTTCGTTTTCCGAAGCACTGCGTGCCGGTACCGAGAT
CTTTCATGCGCTAAAGTCAGTGCTGAAGGGACAGGGTTTAAGTACTGCGGTGGGTGATGAAGGGGGCTTTGCTCCGGACT
TCCGCAGTAATGTTGAGGCACTGGATGCCATTCTTGAGGCAATAGGTAGGGCTGGTTATATCGCTGGAGAGGATGTGTTG
TTAGGTTTGGATGTTGCTTCTTCCGAGTTTCGCAACAACGGTAAGTACAACTTGGTTGGTGAGAACAAGCGTCTGACCAG
TGAGCAGTTTGTTGATTTCTTGGATGATTTGGTTGCGCAGTACCCAATCATCAGTATTGAGGATGGTTTAGCCGAGGATG
ATTGGGTGGGTTGGAAGCAATTGACTGAACGTATCGGCCATAAAGTACAGTTGGTGGGCGATGATCTGTTTGTGACTAAT
CCTAAGGTTTTCCAGGAAGGCATCACTTCTGGCATCGCGAATGCGATCCTTATAAAACTTAACCAAATCGGTACGTTGAC
GGAGACTCTGGAATCAATTGCGATGGCTCACCGTGCTCAGTACGCTGCTATCGTCTCGCATCGATCAGGCGAGACTGAGG
ATACATCAATTGCGGACATTGCGGTGGCGACTACGGCAACTCAAATCAAGACTGGCTCGTTATGCCGTTCTGATCGTGTT
GCCAAGTACAACCAGTTGTTACGCATTGAGCAGGCGCTTGGTGTTGGTGCGCGTTATGCTGGTCGTGATGCGTTCGTTTC
CCTGAAGTCCTGA

Upstream 100 bases:

>100_bases
AGATCTTTTGGGGCCGGAGCTATTTTGCGAGTCGCGCTACCGCGATGATAATCCGATGGTTCTCTGGTATTGCTCCCTTC
TTTGGTTGTTGACTGGACCC

Downstream 100 bases:

>100_bases
TTTGTGCGTAACTGGCGCTGGTTATTGCTGGTGCTTGCGGCATTGCTGGCTTGGTTACAGCACCGCTTCTGGTTTGGCCC
TGGTAACTCCGGGGAGGTCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTVIAKIYAREILDSRGNPTLEAEVTLENAVCGRAAVPSGASTGTKEAVELRDGDKTRYLGKGVRAAVDNVNGVIAAALV
GFDGADQTGLDHRLINLDGTENKGRLGANALLGVSLATAHAVAAARKQPLWMYLSTLGESKLSLPVPMMNIINGGAHADN
NVDFQEFMVLPVGFASFSEALRAGTEIFHALKSVLKGQGLSTAVGDEGGFAPDFRSNVEALDAILEAIGRAGYIAGEDVL
LGLDVASSEFRNNGKYNLVGENKRLTSEQFVDFLDDLVAQYPIISIEDGLAEDDWVGWKQLTERIGHKVQLVGDDLFVTN
PKVFQEGITSGIANAILIKLNQIGTLTETLESIAMAHRAQYAAIVSHRSGETEDTSIADIAVATTATQIKTGSLCRSDRV
AKYNQLLRIEQALGVGARYAGRDAFVSLKS

Sequences:

>Translated_430_residues
MTVIAKIYAREILDSRGNPTLEAEVTLENAVCGRAAVPSGASTGTKEAVELRDGDKTRYLGKGVRAAVDNVNGVIAAALV
GFDGADQTGLDHRLINLDGTENKGRLGANALLGVSLATAHAVAAARKQPLWMYLSTLGESKLSLPVPMMNIINGGAHADN
NVDFQEFMVLPVGFASFSEALRAGTEIFHALKSVLKGQGLSTAVGDEGGFAPDFRSNVEALDAILEAIGRAGYIAGEDVL
LGLDVASSEFRNNGKYNLVGENKRLTSEQFVDFLDDLVAQYPIISIEDGLAEDDWVGWKQLTERIGHKVQLVGDDLFVTN
PKVFQEGITSGIANAILIKLNQIGTLTETLESIAMAHRAQYAAIVSHRSGETEDTSIADIAVATTATQIKTGSLCRSDRV
AKYNQLLRIEQALGVGARYAGRDAFVSLKS
>Mature_429_residues
TVIAKIYAREILDSRGNPTLEAEVTLENAVCGRAAVPSGASTGTKEAVELRDGDKTRYLGKGVRAAVDNVNGVIAAALVG
FDGADQTGLDHRLINLDGTENKGRLGANALLGVSLATAHAVAAARKQPLWMYLSTLGESKLSLPVPMMNIINGGAHADNN
VDFQEFMVLPVGFASFSEALRAGTEIFHALKSVLKGQGLSTAVGDEGGFAPDFRSNVEALDAILEAIGRAGYIAGEDVLL
GLDVASSEFRNNGKYNLVGENKRLTSEQFVDFLDDLVAQYPIISIEDGLAEDDWVGWKQLTERIGHKVQLVGDDLFVTNP
KVFQEGITSGIANAILIKLNQIGTLTETLESIAMAHRAQYAAIVSHRSGETEDTSIADIAVATTATQIKTGSLCRSDRVA
KYNQLLRIEQALGVGARYAGRDAFVSLKS

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=51.3953488372093, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=49.7674418604651, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=50.1165501165501, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=50.1165501165501, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=45.9016393442623, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=62.5882352941176, Blast_Score=521, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=51.9721577726218, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=51.9721577726218, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=47.1204188481675, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=50.5773672055427, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.9166666666667, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.9166666666667, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.9166666666667, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=50.1154734411085, Blast_Score=368, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=48.2678983833718, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=48.2678983833718, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=48.2678983833718, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=48.2678983833718, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=48.2678983833718, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=48.2678983833718, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_XYLF2 (B2I937)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829286.1
- ProteinModelPortal:   B2I937
- SMR:   B2I937
- GeneID:   6203023
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0568
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45747; Mature: 45616

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 339-339 BINDING 157-157 BINDING 166-166 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIAKIYAREILDSRGNPTLEAEVTLENAVCGRAAVPSGASTGTKEAVELRDGDKTRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECHHCCCCCCCCCCCCCCCCHHHEECCCCCHHHH
GKGVRAAVDNVNGVIAAALVGFDGADQTGLDHRLINLDGTENKGRLGANALLGVSLATAH
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
AVAAARKQPLWMYLSTLGESKLSLPVPMMNIINGGAHADNNVDFQEFMVLPVGFASFSEA
HHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHH
LRAGTEIFHALKSVLKGQGLSTAVGDEGGFAPDFRSNVEALDAILEAIGRAGYIAGEDVL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEE
LGLDVASSEFRNNGKYNLVGENKRLTSEQFVDFLDDLVAQYPIISIEDGLAEDDWVGWKQ
EEEECHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHH
LTERIGHKVQLVGDDLFVTNPKVFQEGITSGIANAILIKLNQIGTLTETLESIAMAHRAQ
HHHHHCCEEEEECCCEEECCHHHHHHHHHHHHHHEEEEEEHHCCHHHHHHHHHHHHHHHH
YAAIVSHRSGETEDTSIADIAVATTATQIKTGSLCRSDRVAKYNQLLRIEQALGVGARYA
HHHHHHCCCCCCCCCCHHHEEEEHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCC
GRDAFVSLKS
CCCCEEEECC
>Mature Secondary Structure 
TVIAKIYAREILDSRGNPTLEAEVTLENAVCGRAAVPSGASTGTKEAVELRDGDKTRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECHHCCCCCCCCCCCCCCCCHHHEECCCCCHHHH
GKGVRAAVDNVNGVIAAALVGFDGADQTGLDHRLINLDGTENKGRLGANALLGVSLATAH
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
AVAAARKQPLWMYLSTLGESKLSLPVPMMNIINGGAHADNNVDFQEFMVLPVGFASFSEA
HHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHH
LRAGTEIFHALKSVLKGQGLSTAVGDEGGFAPDFRSNVEALDAILEAIGRAGYIAGEDVL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEE
LGLDVASSEFRNNGKYNLVGENKRLTSEQFVDFLDDLVAQYPIISIEDGLAEDDWVGWKQ
EEEECHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHH
LTERIGHKVQLVGDDLFVTNPKVFQEGITSGIANAILIKLNQIGTLTETLESIAMAHRAQ
HHHHHCCEEEEECCCEEECCHHHHHHHHHHHHHHEEEEEEHHCCHHHHHHHHHHHHHHHH
YAAIVSHRSGETEDTSIADIAVATTATQIKTGSLCRSDRVAKYNQLLRIEQALGVGARYA
HHHHHHCCCCCCCCCCHHHEEEEHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCC
GRDAFVSLKS
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA