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The map label for this gene is lipB

Identifier: 182681113

GI number: 182681113

Start: 662102

End: 662782

Strand: Reverse

Name: lipB

Synonym: XfasM23_0555

Alternate gene names: 182681113

Gene position: 662782-662102 (Counterclockwise)

Preceding gene: 182681114

Following gene: 182681112

Centisome position: 26.14

GC content: 58.15

Gene sequence:

>681_bases
GTGAATGCGTGTGGTGCTGTTGCGCCCTGGCATGTTGGCCGTGCCGCGCTGCTGCGTATGCTGGGTGCTCAGCCTTATGT
GCCGGTTTGGCATGCGATGCAACGTTTTACGGATGTACGCGATGCTACTGCGGTTGACGAGCTGTGGGTAGTTGAGCATG
AGCCGGTATTCACTCTTGGGCAGGCTGGGAAGCTTGAGCATGTCCTGGCCCCTGGGGAGATCCCGGTGGTGCACGTGGAC
CGCGGTGGTCAGGTGACCTATCACGGTCCTGGTCAATTGGTGGTGTATCCACTGCTGGATCTATCCCGTCTGGGGCTTGG
TGTCCGTGATTATGTTTATGGGATTGAGCAGGCGGTGATTAATACGCTGGCTCAGTGGAATATTCTCGGTGAGCGGCGCG
AGCATGCTCCTGGGGTCTATGTCGGTGACGCCAAAATTGCCGCGCTGGGTATTCGGGTACGCCGTGGTTGCAGTTTCCAC
GGTGTGGCGTTCAACGTTGCAATGGATTTGGAGCCGTTCCATCGTATTCATCCTTGTGGTTACCGGGGGCTGCAGGTGAC
CTCGGTGTTAGACTTGGGGGGTCCTTCCGAGATGGACACGGTGGCGGCTGCATTGCTCGCTGAGCTAGCGCGGCAATTTG
GTTTCGTGCTGCACCCCACGTCCGGATGGCTTAGTTCTTAG

Upstream 100 bases:

>100_bases
AGTATGTGTCGGTGCGGATTGGCTTCCGTGCGCTGGATCGGGAGCAATATGACGCTGCTCACCAAGTGCTGCGTGATCAT
CCGGAAGTGAAGTGGACGCT

Downstream 100 bases:

>100_bases
TTTTTTGCAAGCGGTCTAGCCTATTCTGGTATTGCGATGACTCAACCTATTATCCGTTCTATCCCGTTGCATGTTGTCTC
AAATGATCATCCTTCATCTT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLGQAGKLEHVLAPGEIPVVHVD
RGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFH
GVAFNVAMDLEPFHRIHPCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS

Sequences:

>Translated_226_residues
MNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLGQAGKLEHVLAPGEIPVVHVD
RGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFH
GVAFNVAMDLEPFHRIHPCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS
>Mature_226_residues
MNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLGQAGKLEHVLAPGEIPVVHVD
RGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVINTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFH
GVAFNVAMDLEPFHRIHPCGYRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=112, Percent_Identity=43.75, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI87081767, Length=193, Percent_Identity=56.4766839378238, Blast_Score=233, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6323268, Length=199, Percent_Identity=30.1507537688442, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21355483, Length=174, Percent_Identity=37.9310344827586, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24586; Mature: 24586

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLG
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEC
QAGKLEHVLAPGEIPVVHVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVI
CCCCEEEEECCCCEEEEEECCCCEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHHHH
NTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFHGVAFNVAMDLEPFHRIHPCG
HHHHHHHHHCCHHHCCCCEEECCCEEEEEEEHHHCCCCCCCEEEEEEECCCHHHHCCCCC
YRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCC
>Mature Secondary Structure
MNACGAVAPWHVGRAALLRMLGAQPYVPVWHAMQRFTDVRDATAVDELWVVEHEPVFTLG
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEC
QAGKLEHVLAPGEIPVVHVDRGGQVTYHGPGQLVVYPLLDLSRLGLGVRDYVYGIEQAVI
CCCCEEEEECCCCEEEEEECCCCEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHHHH
NTLAQWNILGERREHAPGVYVGDAKIAALGIRVRRGCSFHGVAFNVAMDLEPFHRIHPCG
HHHHHHHHHCCHHHCCCCEEECCCEEEEEEEHHHCCCCCCCEEEEEEECCCHHHHCCCCC
YRGLQVTSVLDLGGPSEMDTVAAALLAELARQFGFVLHPTSGWLSS
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]