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The map label for this gene is lipA

Identifier: 182681112

GI number: 182681112

Start: 660944

End: 662065

Strand: Reverse

Name: lipA

Synonym: XfasM23_0554

Alternate gene names: 182681112

Gene position: 662065-660944 (Counterclockwise)

Preceding gene: 182681113

Following gene: 182681111

Centisome position: 26.11

GC content: 53.21

Gene sequence:

>1122_bases
ATGACTCAACCTATTATCCGTTCTATCCCGTTGCATGTTGTCTCAAATGATCATCCTTCATCTTCCCCATTGCAGCCTGG
AGTAAAGCAATCGGGTGAAGACAAGATTGGGCGTTCGCCTGTGCAGTTTGCAGATGTTCCGGTGTTACGTAAGCCATCAT
GGATTCGCGTTCGCATTCCATCCGGCAATGCGGTGCAAAGTCTCAAAGCCAAGCTACGTGAGAATCGTTTAGTCACGGTG
TGTGAGGAGGCTGCCTGCCCTAATATCCATGAGTGTTTTAGCCATGGCACAGCGACGTTCATGATTCTGGGTGAAGTGTG
CACGCGTCGTTGTTCGTTTTGCGATGTGGCGCATGGCCGCCCCAAACCGCCGGACCCGGAGGAACCAATCAGTTTGGCAC
GCACCGTCGCTGAGATGGGTTTGAAGTACGTGGTGGTGACCAGTGTGGATCGCGATGATTTGCGTGACGGTGGTGCCCAG
CATTTTGTTGATTGCATTGCTGCGATCCGTCAGAGTGCACCACAAACACGGATCGAGATTCTGACCCCAGATTTCCGAGG
AAAGGGACGCATGGATCGTGCATTGGACATTTTGGCGGCATGTCCGCCGGATGTATTCAATCATAACGTTGAAACGGTGC
CCGCTTTGTATCCCAATGTGCGACCCGGAGCGGATTATCAATGGTCATTGACGCTCTTAAAACGCTTTAAGGCACAGCAT
CCGCAGGTGCCTACGAAGTCCGGGATCATGCTCGGTTTAGGGGAGACTCTGGATCAGGTACAGGCGACGTTGCGTGACTT
GCGTGCTCATGACGTGGATATGGTCACGGTTGGTCAGTATCTGCAGCCGACGCCGCATCATCACCCAGTGTTGCGGTATT
GGACTCCGGATGAGTACAAGGCGTTGGAAGAGTACGGTATGGCGTTGGGTTTCAGCCACGTTGCTTCAGGTCCGATGGTG
CGTTCTTCGTATCACGCCGACCATCAGGCGAAAGAGGCGGGCTTAGGCTTCAATGCCACCGTTTCGCTGGGCTCTCCAGC
GGTATCGTCGACTGAACATCGGGAGCGCAATACCATCGCATCAAAATCAGCCTCTAAGACTGAATCGATCCATCATAGAT
AG

Upstream 100 bases:

>100_bases
CGCTGAGCTAGCGCGGCAATTTGGTTTCGTGCTGCACCCCACGTCCGGATGGCTTAGTTCTTAGTTTTTTGCAAGCGGTC
TAGCCTATTCTGGTATTGCG

Downstream 100 bases:

>100_bases
CTCAGAATGCGTACCACCAGTCCAGAAACAGTCAGAACACCCGCGCATTCTCTCGTGTAGTCAGGTTGCGTTTGGAGGAT
GAGGCGTTGCACGAAACAAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTQPIIRSIPLHVVSNDHPSSSPLQPGVKQSGEDKIGRSPVQFADVPVLRKPSWIRVRIPSGNAVQSLKAKLRENRLVTV
CEEAACPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDPEEPISLARTVAEMGLKYVVVTSVDRDDLRDGGAQ
HFVDCIAAIRQSAPQTRIEILTPDFRGKGRMDRALDILAACPPDVFNHNVETVPALYPNVRPGADYQWSLTLLKRFKAQH
PQVPTKSGIMLGLGETLDQVQATLRDLRAHDVDMVTVGQYLQPTPHHHPVLRYWTPDEYKALEEYGMALGFSHVASGPMV
RSSYHADHQAKEAGLGFNATVSLGSPAVSSTEHRERNTIASKSASKTESIHHR

Sequences:

>Translated_373_residues
MTQPIIRSIPLHVVSNDHPSSSPLQPGVKQSGEDKIGRSPVQFADVPVLRKPSWIRVRIPSGNAVQSLKAKLRENRLVTV
CEEAACPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDPEEPISLARTVAEMGLKYVVVTSVDRDDLRDGGAQ
HFVDCIAAIRQSAPQTRIEILTPDFRGKGRMDRALDILAACPPDVFNHNVETVPALYPNVRPGADYQWSLTLLKRFKAQH
PQVPTKSGIMLGLGETLDQVQATLRDLRAHDVDMVTVGQYLQPTPHHHPVLRYWTPDEYKALEEYGMALGFSHVASGPMV
RSSYHADHQAKEAGLGFNATVSLGSPAVSSTEHRERNTIASKSASKTESIHHR
>Mature_372_residues
TQPIIRSIPLHVVSNDHPSSSPLQPGVKQSGEDKIGRSPVQFADVPVLRKPSWIRVRIPSGNAVQSLKAKLRENRLVTVC
EEAACPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDPEEPISLARTVAEMGLKYVVVTSVDRDDLRDGGAQH
FVDCIAAIRQSAPQTRIEILTPDFRGKGRMDRALDILAACPPDVFNHNVETVPALYPNVRPGADYQWSLTLLKRFKAQHP
QVPTKSGIMLGLGETLDQVQATLRDLRAHDVDMVTVGQYLQPTPHHHPVLRYWTPDEYKALEEYGMALGFSHVASGPMVR
SSYHADHQAKEAGLGFNATVSLGSPAVSSTEHRERNTIASKSASKTESIHHR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=49.1228070175439, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=48.5943775100402, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1786846, Length=312, Percent_Identity=60.8974358974359, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=46.3667820069204, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=48.936170212766, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=47.3684210526316, Blast_Score=260, Evalue=9e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_XYLF2 (B2I923)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829272.1
- ProteinModelPortal:   B2I923
- SMR:   B2I923
- GeneID:   6204121
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0554
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 41126; Mature: 40995

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPIIRSIPLHVVSNDHPSSSPLQPGVKQSGEDKIGRSPVQFADVPVLRKPSWIRVRIP
CCCCHHHHCCEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
SGNAVQSLKAKLRENRLVTVCEEAACPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGR
CCCHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCC
PKPPDPEEPISLARTVAEMGLKYVVVTSVDRDDLRDGGAQHFVDCIAAIRQSAPQTRIEI
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
LTPDFRGKGRMDRALDILAACPPDVFNHNVETVPALYPNVRPGADYQWSLTLLKRFKAQH
ECCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCC
PQVPTKSGIMLGLGETLDQVQATLRDLRAHDVDMVTVGQYLQPTPHHHPVLRYWTPDEYK
CCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEECCCHHHH
ALEEYGMALGFSHVASGPMVRSSYHADHQAKEAGLGFNATVSLGSPAVSSTEHRERNTIA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHH
SKSASKTESIHHR
HCCCHHHHHHCCC
>Mature Secondary Structure 
TQPIIRSIPLHVVSNDHPSSSPLQPGVKQSGEDKIGRSPVQFADVPVLRKPSWIRVRIP
CCCHHHHCCEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
SGNAVQSLKAKLRENRLVTVCEEAACPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGR
CCCHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCC
PKPPDPEEPISLARTVAEMGLKYVVVTSVDRDDLRDGGAQHFVDCIAAIRQSAPQTRIEI
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
LTPDFRGKGRMDRALDILAACPPDVFNHNVETVPALYPNVRPGADYQWSLTLLKRFKAQH
ECCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCC
PQVPTKSGIMLGLGETLDQVQATLRDLRAHDVDMVTVGQYLQPTPHHHPVLRYWTPDEYK
CCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEECCCHHHH
ALEEYGMALGFSHVASGPMVRSSYHADHQAKEAGLGFNATVSLGSPAVSSTEHRERNTIA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHH
SKSASKTESIHHR
HCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA