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The map label for this gene is eda [H]

Identifier: 182680896

GI number: 182680896

Start: 431717

End: 432376

Strand: Reverse

Name: eda [H]

Synonym: XfasM23_0334

Alternate gene names: 182680896

Gene position: 432376-431717 (Counterclockwise)

Preceding gene: 182680897

Following gene: 182680894

Centisome position: 17.05

GC content: 56.21

Gene sequence:

>660_bases
ATGAGCATTACTGAAACCCAAAACAAAGCCGAATCCCTGTTATACAACGCCGGCATCCTACCTGTTGTAACCGTGGACAC
AGTGGATCAGGCGCGCCGTGTCGTCGGCGCACTGCTGGAAGGCGGCCTCACCACGATCGAACTGACCTTACGCACCCCAG
TGGCCCTGGAAGCATTAGCAACTCTCAAGCGCGAACAACCAGAAATCACGATCGGAGCAGGGACCGTACTCAACGAACAC
CAACTCCGCCAATCAATCGACGCTGGAGCCGACTTTCTTATTACACCAGGCACCCCTCCCACCTTGGCACAGCTGCTAGC
CGATGCACCAATCCCCGCAGTACCGGGTGCGGCCACAGCAACAGAATTGCTCGCACTCATGGTGTTGGGCTTCCACGTCT
GCAAACTGTTTCCGGCCAATGTGGTGGGCGGCCTGCACATGCTCACCAGCTTGGCCGGCCCCCTAACCAATCTGAAACTA
TGCCCCACTGGTGGTATCAACGAAACCAATGCTAGCGACTATCTGGCACAACCCAACGTCATCTGCATCGGCGGCTCATG
GATGGTTCCCAAAGACTGGCTCGTTCAAGGGCATTGGGACAACATCAAAAACTCCTCCTCAAAGGCCGCAGCAATCATCC
AACAAGCGCGCAGCCACTAA

Upstream 100 bases:

>100_bases
AAGCCCCCTGAGACATCACCCAATACACAGCCCCAAATAGCACACACCGCTTGCCCTGAATCCCCTGTCCTATTCGACAT
CGCCCTATTTAAAGACACAA

Downstream 100 bases:

>100_bases
ATATCGATTCATCTGCAGATCTAGGGACATATCTGTAATCCCATGCCCAGTAGCCACTTATCGAAAAAGTAATCTGAAAA
ATCTGTTGCAGTTTTAGCGA

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSITETQNKAESLLYNAGILPVVTVDTVDQARRVVGALLEGGLTTIELTLRTPVALEALATLKREQPEITIGAGTVLNEH
QLRQSIDAGADFLITPGTPPTLAQLLADAPIPAVPGAATATELLALMVLGFHVCKLFPANVVGGLHMLTSLAGPLTNLKL
CPTGGINETNASDYLAQPNVICIGGSWMVPKDWLVQGHWDNIKNSSSKAAAIIQQARSH

Sequences:

>Translated_219_residues
MSITETQNKAESLLYNAGILPVVTVDTVDQARRVVGALLEGGLTTIELTLRTPVALEALATLKREQPEITIGAGTVLNEH
QLRQSIDAGADFLITPGTPPTLAQLLADAPIPAVPGAATATELLALMVLGFHVCKLFPANVVGGLHMLTSLAGPLTNLKL
CPTGGINETNASDYLAQPNVICIGGSWMVPKDWLVQGHWDNIKNSSSKAAAIIQQARSH
>Mature_218_residues
SITETQNKAESLLYNAGILPVVTVDTVDQARRVVGALLEGGLTTIELTLRTPVALEALATLKREQPEITIGAGTVLNEHQ
LRQSIDAGADFLITPGTPPTLAQLLADAPIPAVPGAATATELLALMVLGFHVCKLFPANVVGGLHMLTSLAGPLTNLKLC
PTGGINETNASDYLAQPNVICIGGSWMVPKDWLVQGHWDNIKNSSSKAAAIIQQARSH

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=203, Percent_Identity=41.3793103448276, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 23026; Mature: 22895

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITETQNKAESLLYNAGILPVVTVDTVDQARRVVGALLEGGLTTIELTLRTPVALEALA
CCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHH
TLKREQPEITIGAGTVLNEHQLRQSIDAGADFLITPGTPPTLAQLLADAPIPAVPGAATA
HHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCCCCCCHHH
TELLALMVLGFHVCKLFPANVVGGLHMLTSLAGPLTNLKLCPTGGINETNASDYLAQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHCCCCE
ICIGGSWMVPKDWLVQGHWDNIKNSSSKAAAIIQQARSH
EEECCCEECCHHHEEECCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SITETQNKAESLLYNAGILPVVTVDTVDQARRVVGALLEGGLTTIELTLRTPVALEALA
CCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHH
TLKREQPEITIGAGTVLNEHQLRQSIDAGADFLITPGTPPTLAQLLADAPIPAVPGAATA
HHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCCCCCCHHH
TELLALMVLGFHVCKLFPANVVGGLHMLTSLAGPLTNLKLCPTGGINETNASDYLAQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHCCCCE
ICIGGSWMVPKDWLVQGHWDNIKNSSSKAAAIIQQARSH
EEECCCEECCHHHEEECCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]