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The map label for this gene is proB

Identifier: 182680853

GI number: 182680853

Start: 374057

End: 375211

Strand: Direct

Name: proB

Synonym: XfasM23_0290

Alternate gene names: 182680853

Gene position: 374057-375211 (Clockwise)

Preceding gene: 182680852

Following gene: 182680854

Centisome position: 14.75

GC content: 62.77

Gene sequence:

>1155_bases
ATGACCGGCATCCCTCCCCCCTCCCGCTTCCCCGAACAACCCATCCCCCCGTGGCGCCGCGCCGTCCTCAAAGTGGGTAG
CAGCCTCCTCGCCGCCGATGGCGGCGGCCTTTCCCCCCGCTTTGCCTTGGACTTAGCACACTTCGTCTCCGCCAACATCA
CCGCCGGACGCCAACTCGTCATCGTCTCCTCTGGCGCTGTCGCCGCTGGTCGTGCCCTGATACCCCCACTGCCCGAATCC
GGCGGCGCTCTCGCCGCCCGCCAAGCCTTAGCTGCCCTTGGACAAGCACAACTCATCGCACTGTGGCAACGCTTCTTCGA
CCGCCCCGTTGCCCAGGTCCTCCTGACCCACGACGACCTCCGCAACCGCCGTCGCTACCTCAACGCACGTGCCACATTGC
GTGAACTACTGCACCTGGGCACCTTGCCTGTCGTCAACGAAAACGACACCGTCTCCGTCGATGAACTCAAACTAGGCGAC
AACGACAACCTGGCAGCCATTGTTGCCGCACTGATTGATGCCCAAGCCCTGTTTATCGCCACCGATATTGACGGCTTATA
CACCACCGACCCACGCCACCATTCTGACGCACAGCCCCTGCATGAAGTCCGCACCCTGACCCCTGAAAACCTTGCCATGG
CTGGTGACAGCAGCAGCACCGTTGGCACCGGCGGTATGCGTACCAAACTTGAAGCAGCACTTAAAGCCGGCGCCGCTGGC
ATTGACACCTACCTATTTAACGGCCGCAGCAGCGACGTTGTCCGCGGACTCGCACAACACCGCCTCCGCGGCACCCGCAT
CCATCCCACCTGTACCCCGATTGCCGCCCGTAAATATTGGTTGCGTCACGCCCCAGTGGAGCCCGGCGCGATCCTCATCG
ATGCCGGTGCCGCCGCTGCCTTGGCACAACAAGGCGCTTCCCTACTTCCGGGCGGCGTTTTGAGTGCCGAAGGCGACTTC
CGCCGCGGCGATATGATCCAGATAGCCACCCGCAGCCCAGATCACCCCTCCCACCCCCTGGCACGCGGACTTGTCCAATA
CTCAGCCGCCGATGTCCGCCGCATTGCCGGTTGCCATTCCCGCGATATTCAAACCCTCCTTGGATACACCTATGGCGACA
CAATCGTTCACCGTGATGACCTCGTCCTCCTCTGA

Upstream 100 bases:

>100_bases
CCATCACCCCCCCCCACACACACCAACCCATCCCCTCCCTGACCCCCCACAGCAACTGCCCATGCCTCCCATCTATCCCT
GCAACTGACCCCGTCTCCCC

Downstream 100 bases:

>100_bases
CCGCCCCCCACTCTTGCAACCATCACCACCATCAAATAAATAGCGAACCGCAACTGACCTCCCGATAGCCAGCCCCCCAT
ACCATTGCACCCCCTGCCTA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTGIPPPSRFPEQPIPPWRRAVLKVGSSLLAADGGGLSPRFALDLAHFVSANITAGRQLVIVSSGAVAAGRALIPPLPES
GGALAARQALAALGQAQLIALWQRFFDRPVAQVLLTHDDLRNRRRYLNARATLRELLHLGTLPVVNENDTVSVDELKLGD
NDNLAAIVAALIDAQALFIATDIDGLYTTDPRHHSDAQPLHEVRTLTPENLAMAGDSSSTVGTGGMRTKLEAALKAGAAG
IDTYLFNGRSSDVVRGLAQHRLRGTRIHPTCTPIAARKYWLRHAPVEPGAILIDAGAAAALAQQGASLLPGGVLSAEGDF
RRGDMIQIATRSPDHPSHPLARGLVQYSAADVRRIAGCHSRDIQTLLGYTYGDTIVHRDDLVLL

Sequences:

>Translated_384_residues
MTGIPPPSRFPEQPIPPWRRAVLKVGSSLLAADGGGLSPRFALDLAHFVSANITAGRQLVIVSSGAVAAGRALIPPLPES
GGALAARQALAALGQAQLIALWQRFFDRPVAQVLLTHDDLRNRRRYLNARATLRELLHLGTLPVVNENDTVSVDELKLGD
NDNLAAIVAALIDAQALFIATDIDGLYTTDPRHHSDAQPLHEVRTLTPENLAMAGDSSSTVGTGGMRTKLEAALKAGAAG
IDTYLFNGRSSDVVRGLAQHRLRGTRIHPTCTPIAARKYWLRHAPVEPGAILIDAGAAAALAQQGASLLPGGVLSAEGDF
RRGDMIQIATRSPDHPSHPLARGLVQYSAADVRRIAGCHSRDIQTLLGYTYGDTIVHRDDLVLL
>Mature_383_residues
TGIPPPSRFPEQPIPPWRRAVLKVGSSLLAADGGGLSPRFALDLAHFVSANITAGRQLVIVSSGAVAAGRALIPPLPESG
GALAARQALAALGQAQLIALWQRFFDRPVAQVLLTHDDLRNRRRYLNARATLRELLHLGTLPVVNENDTVSVDELKLGDN
DNLAAIVAALIDAQALFIATDIDGLYTTDPRHHSDAQPLHEVRTLTPENLAMAGDSSSTVGTGGMRTKLEAALKAGAAGI
DTYLFNGRSSDVVRGLAQHRLRGTRIHPTCTPIAARKYWLRHAPVEPGAILIDAGAAAALAQQGASLLPGGVLSAEGDFR
RGDMIQIATRSPDHPSHPLARGLVQYSAADVRRIAGCHSRDIQTLLGYTYGDTIVHRDDLVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=263, Percent_Identity=29.6577946768061, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI62912457, Length=261, Percent_Identity=29.8850574712644, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.896174863388, Blast_Score=265, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17569967, Length=255, Percent_Identity=34.9019607843137, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=255, Percent_Identity=34.9019607843137, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=34.9282296650718, Blast_Score=215, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6321822, Length=416, Percent_Identity=33.8942307692308, Blast_Score=196, Evalue=7e-51,
Organism=Drosophila melanogaster, GI21357643, Length=281, Percent_Identity=31.6725978647687, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_XYLF2 (B2I7L1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829013.1
- ProteinModelPortal:   B2I7L1
- SMR:   B2I7L1
- GeneID:   6203961
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0290
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40872; Mature: 40741

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIPPPSRFPEQPIPPWRRAVLKVGSSLLAADGGGLSPRFALDLAHFVSANITAGRQLV
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHEECCCCCCCCHHHHHHHHHHHCCCCCCCEEE
IVSSGAVAAGRALIPPLPESGGALAARQALAALGQAQLIALWQRFFDRPVAQVLLTHDDL
EEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHH
RNRRRYLNARATLRELLHLGTLPVVNENDTVSVDELKLGDNDNLAAIVAALIDAQALFIA
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEEECCCCCHHHHHHHHHCCCEEEEE
TDIDGLYTTDPRHHSDAQPLHEVRTLTPENLAMAGDSSSTVGTGGMRTKLEAALKAGAAG
ECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCC
IDTYLFNGRSSDVVRGLAQHRLRGTRIHPTCTPIAARKYWLRHAPVEPGAILIDAGAAAA
CHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHH
LAQQGASLLPGGVLSAEGDFRRGDMIQIATRSPDHPSHPLARGLVQYSAADVRRIAGCHS
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
RDIQTLLGYTYGDTIVHRDDLVLL
HHHHHHHCCCCCCEEEECCCEEEC
>Mature Secondary Structure 
TGIPPPSRFPEQPIPPWRRAVLKVGSSLLAADGGGLSPRFALDLAHFVSANITAGRQLV
CCCCCCCCCCCCCCCHHHHHHHHHCCHHEECCCCCCCCHHHHHHHHHHHCCCCCCCEEE
IVSSGAVAAGRALIPPLPESGGALAARQALAALGQAQLIALWQRFFDRPVAQVLLTHDDL
EEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHH
RNRRRYLNARATLRELLHLGTLPVVNENDTVSVDELKLGDNDNLAAIVAALIDAQALFIA
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEEECCCCCHHHHHHHHHCCCEEEEE
TDIDGLYTTDPRHHSDAQPLHEVRTLTPENLAMAGDSSSTVGTGGMRTKLEAALKAGAAG
ECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCC
IDTYLFNGRSSDVVRGLAQHRLRGTRIHPTCTPIAARKYWLRHAPVEPGAILIDAGAAAA
CHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHH
LAQQGASLLPGGVLSAEGDFRRGDMIQIATRSPDHPSHPLARGLVQYSAADVRRIAGCHS
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
RDIQTLLGYTYGDTIVHRDDLVLL
HHHHHHHCCCCCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA