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The map label for this gene is panB

Identifier: 182680744

GI number: 182680744

Start: 236668

End: 237486

Strand: Direct

Name: panB

Synonym: XfasM23_0173

Alternate gene names: 182680744

Gene position: 236668-237486 (Clockwise)

Preceding gene: 182680743

Following gene: 182680745

Centisome position: 9.33

GC content: 54.7

Gene sequence:

>819_bases
ATGAGCATATATACCAATAGCAAACCTTGGACGGTGCCTGCTTTGGCGGAGGCCAAGCGAAACGGTTCTAAAATCGTGAT
GTTGACTGCTTATGATGCTGGGTTTGCTCGTATTCTTGACGCAAACGGCGTAGACCTAATCCTTGTTGGCGATTCGTTGG
GAATGGTGGTACAGGGCCATGACTCGACTCTTCCGGTGAGTGTGCATGACATGGTGTACCACACTGCGTGTGTGGCTCGG
GGGGTACGTCATGCAATGCTTGTGGTTGATTTGCCATTTCAGGCTGATGCGTCGCCGGAACGTGCGCTAGAGGCTGCCAC
TGCATTGCTGCGAGTGGGGGCACAGATGATCAAAATCGAAGGTGCTGGGCACAAGCTTGAGGTGATTAGTTACTTGGTTG
AGCGTGAGATTCCGGTGTGTTCACACTTGGGGCTGACTCCGCAATCGGTACTGCGTTTTGGAGGTTATAAGGTGCAAGGA
CGTGGTGAGGAGGCTGGGGGGCGTTTGCGTGCCGAGGCACGTGCGGCGGTGGAGGCTGGGGCTACTCTCTTGCTATTGGA
GTGTGTGCCGTCGCAGTTGGCAGCGCAGATCACTACCGATGCATCGGTGCCGACGATTGGTATCGGTGCCGGTGCTGGTT
GTGATGGCCAAGTGCTGGTGCTGCATGATCTCCTTGGTCTAGATAGCGGTCACCCTCGACCGAAATTCGTTAAAGATTTT
CTCGCTCATGGGGGATCGGTTGCTGGCGCGGTTCGCGCTTATGCGAATGCGGTTCGAGATGGTTCGTTTCCAGATGTCGA
GCATACTTACACATCGTGA

Upstream 100 bases:

>100_bases
CTCGTCATGGGATGGTATGTGAGCTATTGGAGCATATGGATACCTTGGGCTTGGTACCAATCAGGTAGATAATGTTCACT
GGTTAGATTTTCATGAATTT

Downstream 100 bases:

>100_bases
TTGATACCGTTACTGATCTGTCCAGGCTACGGGGGATTGTTGCTGACTGGAGGCGTCAGGGCTTGCGGGTCGCTCTGGTG
CCTACCATGGGTAATTTGCA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSIYTNSKPWTVPALAEAKRNGSKIVMLTAYDAGFARILDANGVDLILVGDSLGMVVQGHDSTLPVSVHDMVYHTACVAR
GVRHAMLVVDLPFQADASPERALEAATALLRVGAQMIKIEGAGHKLEVISYLVEREIPVCSHLGLTPQSVLRFGGYKVQG
RGEEAGGRLRAEARAAVEAGATLLLLECVPSQLAAQITTDASVPTIGIGAGAGCDGQVLVLHDLLGLDSGHPRPKFVKDF
LAHGGSVAGAVRAYANAVRDGSFPDVEHTYTS

Sequences:

>Translated_272_residues
MSIYTNSKPWTVPALAEAKRNGSKIVMLTAYDAGFARILDANGVDLILVGDSLGMVVQGHDSTLPVSVHDMVYHTACVAR
GVRHAMLVVDLPFQADASPERALEAATALLRVGAQMIKIEGAGHKLEVISYLVEREIPVCSHLGLTPQSVLRFGGYKVQG
RGEEAGGRLRAEARAAVEAGATLLLLECVPSQLAAQITTDASVPTIGIGAGAGCDGQVLVLHDLLGLDSGHPRPKFVKDF
LAHGGSVAGAVRAYANAVRDGSFPDVEHTYTS
>Mature_271_residues
SIYTNSKPWTVPALAEAKRNGSKIVMLTAYDAGFARILDANGVDLILVGDSLGMVVQGHDSTLPVSVHDMVYHTACVARG
VRHAMLVVDLPFQADASPERALEAATALLRVGAQMIKIEGAGHKLEVISYLVEREIPVCSHLGLTPQSVLRFGGYKVQGR
GEEAGGRLRAEARAAVEAGATLLLLECVPSQLAAQITTDASVPTIGIGAGAGCDGQVLVLHDLLGLDSGHPRPKFVKDFL
AHGGSVAGAVRAYANAVRDGSFPDVEHTYTS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=52.851711026616, Blast_Score=281, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6319653, Length=270, Percent_Identity=37.4074074074074, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_XYLF2 (B2I720)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828904.1
- ProteinModelPortal:   B2I720
- SMR:   B2I720
- GeneID:   6202939
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0173
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28543; Mature: 28412

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 90-90 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYTNSKPWTVPALAEAKRNGSKIVMLTAYDAGFARILDANGVDLILVGDSLGMVVQGH
CCCCCCCCCEECHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCEEEEECCCCCEEEECC
DSTLPVSVHDMVYHTACVARGVRHAMLVVDLPFQADASPERALEAATALLRVGAQMIKIE
CCCCCEEHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GAGHKLEVISYLVEREIPVCSHLGLTPQSVLRFGGYKVQGRGEEAGGRLRAEARAAVEAG
CCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCEEEECCCCCCCCEEHHHHHHHHHCC
ATLLLLECVPSQLAAQITTDASVPTIGIGAGAGCDGQVLVLHDLLGLDSGHPRPKFVKDF
CEEEEEECCCHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHH
LAHGGSVAGAVRAYANAVRDGSFPDVEHTYTS
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIYTNSKPWTVPALAEAKRNGSKIVMLTAYDAGFARILDANGVDLILVGDSLGMVVQGH
CCCCCCCCEECHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCEEEEECCCCCEEEECC
DSTLPVSVHDMVYHTACVARGVRHAMLVVDLPFQADASPERALEAATALLRVGAQMIKIE
CCCCCEEHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GAGHKLEVISYLVEREIPVCSHLGLTPQSVLRFGGYKVQGRGEEAGGRLRAEARAAVEAG
CCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCEEEECCCCCCCCEEHHHHHHHHHCC
ATLLLLECVPSQLAAQITTDASVPTIGIGAGAGCDGQVLVLHDLLGLDSGHPRPKFVKDF
CEEEEEECCCHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHH
LAHGGSVAGAVRAYANAVRDGSFPDVEHTYTS
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA