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The map label for this gene is pcnB [H]

Identifier: 182680742

GI number: 182680742

Start: 234852

End: 236036

Strand: Direct

Name: pcnB [H]

Synonym: XfasM23_0171

Alternate gene names: 182680742

Gene position: 234852-236036 (Clockwise)

Preceding gene: 182680741

Following gene: 182680743

Centisome position: 9.26

GC content: 52.07

Gene sequence:

>1185_bases
ATGCTTTGCATCGTACCGCGTGGACACCATACGATTTCACGTAAGGACATTAGTCCGAATGCGTTGCGGGTGTTGTACCG
CCTGCGTGAGGCAGGTTTTGGTGCGTATTTGGTTGGCGGTGCGGTTCGTGATTTGTTGGTAGGCGGTCATCCCAAAGATT
TCGACTTGGCTACCGATGCAACACCTGAGCAGATCAAGCAGTTGTTCAGGAGTTGTCGTTTAATCGGTCGCCGCTTTCGT
TTGGCGCATGTGGTTTTCGGGCGCGAGATTATTGAGGTCGCTACCTTCCGTGCCAATAGTGATGATGGAAGTGGTGATCG
TGAGCTGGAGAATGGCCGTTTAGTGTGTGACAACGTCTACGGTACGATTGAGGACGATGCGACCCGTCGCGATTTCACCT
GTAACGCGCTTTACTACACTATCGAAGATTTTTCAGTACGTGACTACGTCGGTGGTTTTGAGGATGTCCAGTCTCGGCTG
ATGAGATTGATTGGCGATCCAGTGCGACGTTATCAGGAAGATCCAGTGCGCATGCTGCGTGCGGTGCGTTTAGCAGCGAA
GCTCGGTTTCGAGATTGAGGCTGGTACTGCCGAACCAATTTCACGTATGGCTGGATTGTTGGCTGACGTGGCGCCAGCCC
GTTTGTTTGAGGAAGTGCTCAAGCTATTTCTCTCAGGACATGCTGTGGTCAGTTTCGAGGCCTTAGAGGATTTTGGTCTG
ATTGATGTGTTGTTTCCCGAGAGCGCTAAGGCATTGCGTGCTAACCGTAGTGGTGCATTGCGGCGGGTATTGATCGAAGG
GCTGCACAGTACTGATAAGCGGGTGATTAAAGATGAACCAGTGTCCCCGGCATTCTTGTTCGCATTGTTGTTATGGCCTG
GTTTTTGCCATGCGTTGGTCACGTTGCAATTGCAGGGTGTGCAGCCAGAGGAGGCACAACGTCGTGCAGCTGATCGGGTG
ACGTTGCATCATCTTGAGCGTGTCGCTTTGCCGCGCCGCTTTTCTTTGCCGATGCAAGAGATTTGGTTGTTGCAAAACCG
TTTTATTTCACGCCAACCTAAGCGTGTCTTACGGCTTCTGGAACATCCGCGTTTTCGTGCTGCCTTCGATTTCCTGGTAG
TGCGTTTGGCGGCTTCTGCTGAGCATGCTGCAGACGTGGAGTTTTGGCGTGTTGCACAACAGTAG

Upstream 100 bases:

>100_bases
ACCGCTAGACGAAGGGAGCGTTGGTCAGTTCGATAAAGGCCCGTTAATATAAAAGCGCCTTCGCCTGAAGGCAACTCATC
TTTAGGGGCACTATTAGTTC

Downstream 100 bases:

>100_bases
CCTTCTGAAGACTCTCTGGCGTCAGCCATTGATGCTGTGCGGTTTGAATCGGAGGAAGTGGGTGTGTCGCGTCAGCGGTA
ATGCCAAGGTGGTCAAGTAG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLCIVPRGHHTISRKDISPNALRVLYRLREAGFGAYLVGGAVRDLLVGGHPKDFDLATDATPEQIKQLFRSCRLIGRRFR
LAHVVFGREIIEVATFRANSDDGSGDRELENGRLVCDNVYGTIEDDATRRDFTCNALYYTIEDFSVRDYVGGFEDVQSRL
MRLIGDPVRRYQEDPVRMLRAVRLAAKLGFEIEAGTAEPISRMAGLLADVAPARLFEEVLKLFLSGHAVVSFEALEDFGL
IDVLFPESAKALRANRSGALRRVLIEGLHSTDKRVIKDEPVSPAFLFALLLWPGFCHALVTLQLQGVQPEEAQRRAADRV
TLHHLERVALPRRFSLPMQEIWLLQNRFISRQPKRVLRLLEHPRFRAAFDFLVVRLAASAEHAADVEFWRVAQQ

Sequences:

>Translated_394_residues
MLCIVPRGHHTISRKDISPNALRVLYRLREAGFGAYLVGGAVRDLLVGGHPKDFDLATDATPEQIKQLFRSCRLIGRRFR
LAHVVFGREIIEVATFRANSDDGSGDRELENGRLVCDNVYGTIEDDATRRDFTCNALYYTIEDFSVRDYVGGFEDVQSRL
MRLIGDPVRRYQEDPVRMLRAVRLAAKLGFEIEAGTAEPISRMAGLLADVAPARLFEEVLKLFLSGHAVVSFEALEDFGL
IDVLFPESAKALRANRSGALRRVLIEGLHSTDKRVIKDEPVSPAFLFALLLWPGFCHALVTLQLQGVQPEEAQRRAADRV
TLHHLERVALPRRFSLPMQEIWLLQNRFISRQPKRVLRLLEHPRFRAAFDFLVVRLAASAEHAADVEFWRVAQQ
>Mature_394_residues
MLCIVPRGHHTISRKDISPNALRVLYRLREAGFGAYLVGGAVRDLLVGGHPKDFDLATDATPEQIKQLFRSCRLIGRRFR
LAHVVFGREIIEVATFRANSDDGSGDRELENGRLVCDNVYGTIEDDATRRDFTCNALYYTIEDFSVRDYVGGFEDVQSRL
MRLIGDPVRRYQEDPVRMLRAVRLAAKLGFEIEAGTAEPISRMAGLLADVAPARLFEEVLKLFLSGHAVVSFEALEDFGL
IDVLFPESAKALRANRSGALRRVLIEGLHSTDKRVIKDEPVSPAFLFALLLWPGFCHALVTLQLQGVQPEEAQRRAADRV
TLHHLERVALPRRFSLPMQEIWLLQNRFISRQPKRVLRLLEHPRFRAAFDFLVVRLAASAEHAADVEFWRVAQQ

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=200, Percent_Identity=32.5, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI87081691, Length=395, Percent_Identity=50.126582278481, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=34.8623853211009, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=232, Percent_Identity=28.448275862069, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281360112, Length=218, Percent_Identity=32.1100917431193, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357337, Length=218, Percent_Identity=32.1100917431193, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 44636; Mature: 44636

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCIVPRGHHTISRKDISPNALRVLYRLREAGFGAYLVGGAVRDLLVGGHPKDFDLATDA
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TPEQIKQLFRSCRLIGRRFRLAHVVFGREIIEVATFRANSDDGSGDRELENGRLVCDNVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
GTIEDDATRRDFTCNALYYTIEDFSVRDYVGGFEDVQSRLMRLIGDPVRRYQEDPVRMLR
CCCCCCCCCCCEEECEEEEEEECCCHHHHCCCHHHHHHHHHHHHCHHHHHHCCCHHHHHH
AVRLAAKLGFEIEAGTAEPISRMAGLLADVAPARLFEEVLKLFLSGHAVVSFEALEDFGL
HHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCC
IDVLFPESAKALRANRSGALRRVLIEGLHSTDKRVIKDEPVSPAFLFALLLWPGFCHALV
EEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLQLQGVQPEEAQRRAADRVTLHHLERVALPRRFSLPMQEIWLLQNRFISRQPKRVLRLL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
EHPRFRAAFDFLVVRLAASAEHAADVEFWRVAQQ
HCCCHHHHHHHHHHHHHHCCHHHHCHHHHHHCCC
>Mature Secondary Structure
MLCIVPRGHHTISRKDISPNALRVLYRLREAGFGAYLVGGAVRDLLVGGHPKDFDLATDA
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TPEQIKQLFRSCRLIGRRFRLAHVVFGREIIEVATFRANSDDGSGDRELENGRLVCDNVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
GTIEDDATRRDFTCNALYYTIEDFSVRDYVGGFEDVQSRLMRLIGDPVRRYQEDPVRMLR
CCCCCCCCCCCEEECEEEEEEECCCHHHHCCCHHHHHHHHHHHHCHHHHHHCCCHHHHHH
AVRLAAKLGFEIEAGTAEPISRMAGLLADVAPARLFEEVLKLFLSGHAVVSFEALEDFGL
HHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCC
IDVLFPESAKALRANRSGALRRVLIEGLHSTDKRVIKDEPVSPAFLFALLLWPGFCHALV
EEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLQLQGVQPEEAQRRAADRVTLHHLERVALPRRFSLPMQEIWLLQNRFISRQPKRVLRLL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
EHPRFRAAFDFLVVRLAASAEHAADVEFWRVAQQ
HCCCHHHHHHHHHHHHHHCCHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]