Click here to switch to the map view.

The map label for this gene is trpE [H]

Identifier: 182680728

GI number: 182680728

Start: 216182

End: 217687

Strand: Direct

Name: trpE [H]

Synonym: XfasM23_0157

Alternate gene names: 182680728

Gene position: 216182-217687 (Clockwise)

Preceding gene: 182680727

Following gene: 182680729

Centisome position: 8.53

GC content: 50.86

Gene sequence:

>1506_bases
ATGTTATCTGTGTCAGGAAGGAAAGTTGCTTTGATCACTGCAATGCAGTTTCAGCGTCAAGTTGCCGAAGGGTGTACCCG
CATTCCAGTGGTGCGTGAAGTCCAGTCTGATTTGGATACTCCGCTTTCGATCTATTTAAAGTTTGCCGATGCTCCATATA
CCTATTTGTTTGAGTCGGTGGAAGGTGGGGAGCATTTTGCGAGGTATTCGATCATTGGGTTGCCGGCACGCCGTGTCTAT
ACCTTTCATGGGCATACCCTGGTGGTGAGTGAGTTCGGTAAGGTGTTGGAAACGCGTCAAGTCGCCGATCCGCTTGCCGA
AGTTGAGCTGTTGCGTGCGCAGTATGTAGTGCCGCAATTAGAAGGGTTGCCAGGTTTTACAGGCGGCTTAGTGGGGTGGT
TCGGCTTTGAATGTATCCAGTACATCGAACCTCGCTTCATGGGAGGTAAAAAACGTGATGAACTTGGTACGCCAGATATT
CTGCTGATGCTTTCGGAAGAGTTTGCGGTGTTTGACAGCCTCAAGGGTCGATTATATTTGATCGTTCACGCGGATCCTGC
TCAACCGCATTCTTACGTTCGTGCAATGAAACGTTTGGATGCGTTGATCCACCGTCTGCGGCAAGGTGGGATGGATTATC
CGCATTCACATATTTCGGAAGTGATTAACGAGGAAGATTTTCACTCTTCTTTGACGCGTGAGCAGTATCACGCTGTGGTG
CGTAAGGCGCAGGAGTATGTGCGTTCGGGTGATATTTTTCAGGTTGTACCAAGTCAGCGCCTGTGTACGCAGTTTCATGC
ACGTCCGATTGATGTATATCGGGCGTTGCGCACGCTGAATCCGTCGCCTTATATGTATTTCATTGATATTGGTGCTACTC
AGGTGGTTGGTTCATCACCGGAGATATTGGCGCGATTGCAGGGGGATGTGGTCACGGTGCGTCCTATTGCCGGCACACGC
CCGCGAGGTGCGACTCCGGAGCATGACAAACGGTTGGAAATGGAGTTGTTAGCCGATCCAAAAGAGCGTGCTGAGCACGT
GATGCTGATTGATTTGGGTCGCAATGATATCGGACGTGTTGTCGAGGCCGGGAGTGTACAAGTCAAGGAGTCGTTTGTAA
TCGAGCGTTATAGTCATGTCATGCATATTGTCAGTGAGGTCACCGGTATCTTGAAGCCGGGTTTGAACTACGTTGATGTG
TTACGTGCAACGTTCCCTGCGGGTACTGTGTCCGGAGCGCCAAAGATTCGTGCATTGGAGATTATCCGTGAGTTGGAACC
AGTGTGTCGCAACGTATATTCCGGTGCAGTGGGTTACATTGGCTGGCATGGAGATGCTGATACTGCAATCGCGATTCGTA
CTGCGGTGATTCAAAACGGTTGCTTGTACGTGCAGGCTGGCGGTGGTGTGGTGTATGACTCGGATCCAGATTTGGAGTGG
CAGGAAACCATGAACAAGGCGCGTGCATTATTTAGGGCTGTGGCGCAGGCGGCCAGAGGGTTGTGA

Upstream 100 bases:

>100_bases
TCGGTTCTGGTAAAGCGAGCCAGTTTTCACTTGATCAGTCTGTGTTTTTATATGACTTCTCATTTTTTATGGCGATGTTG
ATCCGTTGCCGTTGTCTTCC

Downstream 100 bases:

>100_bases
TGGAGAGTGTCAAAAGTGTATGGGGCCACCACAATAGTGGCAGTGACTACGGTCCATTCACGGATGCTGTTGGTGCTTCA
TTCTTCCGTTATGCGTCTTC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MLSVSGRKVALITAMQFQRQVAEGCTRIPVVREVQSDLDTPLSIYLKFADAPYTYLFESVEGGEHFARYSIIGLPARRVY
TFHGHTLVVSEFGKVLETRQVADPLAEVELLRAQYVVPQLEGLPGFTGGLVGWFGFECIQYIEPRFMGGKKRDELGTPDI
LLMLSEEFAVFDSLKGRLYLIVHADPAQPHSYVRAMKRLDALIHRLRQGGMDYPHSHISEVINEEDFHSSLTREQYHAVV
RKAQEYVRSGDIFQVVPSQRLCTQFHARPIDVYRALRTLNPSPYMYFIDIGATQVVGSSPEILARLQGDVVTVRPIAGTR
PRGATPEHDKRLEMELLADPKERAEHVMLIDLGRNDIGRVVEAGSVQVKESFVIERYSHVMHIVSEVTGILKPGLNYVDV
LRATFPAGTVSGAPKIRALEIIRELEPVCRNVYSGAVGYIGWHGDADTAIAIRTAVIQNGCLYVQAGGGVVYDSDPDLEW
QETMNKARALFRAVAQAARGL

Sequences:

>Translated_501_residues
MLSVSGRKVALITAMQFQRQVAEGCTRIPVVREVQSDLDTPLSIYLKFADAPYTYLFESVEGGEHFARYSIIGLPARRVY
TFHGHTLVVSEFGKVLETRQVADPLAEVELLRAQYVVPQLEGLPGFTGGLVGWFGFECIQYIEPRFMGGKKRDELGTPDI
LLMLSEEFAVFDSLKGRLYLIVHADPAQPHSYVRAMKRLDALIHRLRQGGMDYPHSHISEVINEEDFHSSLTREQYHAVV
RKAQEYVRSGDIFQVVPSQRLCTQFHARPIDVYRALRTLNPSPYMYFIDIGATQVVGSSPEILARLQGDVVTVRPIAGTR
PRGATPEHDKRLEMELLADPKERAEHVMLIDLGRNDIGRVVEAGSVQVKESFVIERYSHVMHIVSEVTGILKPGLNYVDV
LRATFPAGTVSGAPKIRALEIIRELEPVCRNVYSGAVGYIGWHGDADTAIAIRTAVIQNGCLYVQAGGGVVYDSDPDLEW
QETMNKARALFRAVAQAARGL
>Mature_501_residues
MLSVSGRKVALITAMQFQRQVAEGCTRIPVVREVQSDLDTPLSIYLKFADAPYTYLFESVEGGEHFARYSIIGLPARRVY
TFHGHTLVVSEFGKVLETRQVADPLAEVELLRAQYVVPQLEGLPGFTGGLVGWFGFECIQYIEPRFMGGKKRDELGTPDI
LLMLSEEFAVFDSLKGRLYLIVHADPAQPHSYVRAMKRLDALIHRLRQGGMDYPHSHISEVINEEDFHSSLTREQYHAVV
RKAQEYVRSGDIFQVVPSQRLCTQFHARPIDVYRALRTLNPSPYMYFIDIGATQVVGSSPEILARLQGDVVTVRPIAGTR
PRGATPEHDKRLEMELLADPKERAEHVMLIDLGRNDIGRVVEAGSVQVKESFVIERYSHVMHIVSEVTGILKPGLNYVDV
LRATFPAGTVSGAPKIRALEIIRELEPVCRNVYSGAVGYIGWHGDADTAIAIRTAVIQNGCLYVQAGGGVVYDSDPDLEW
QETMNKARALFRAVAQAARGL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=404, Percent_Identity=38.3663366336634, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788114, Length=454, Percent_Identity=32.3788546255507, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082077, Length=278, Percent_Identity=26.978417266187, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1786809, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=494, Percent_Identity=37.0445344129555, Blast_Score=308, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6324361, Length=492, Percent_Identity=26.6260162601626, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 55890; Mature: 55890

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVSGRKVALITAMQFQRQVAEGCTRIPVVREVQSDLDTPLSIYLKFADAPYTYLFESV
CCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHC
EGGEHFARYSIIGLPARRVYTFHGHTLVVSEFGKVLETRQVADPLAEVELLRAQYVVPQL
CCCCHHHHEEEEECCHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLPGFTGGLVGWFGFECIQYIEPRFMGGKKRDELGTPDILLMLSEEFAVFDSLKGRLYL
CCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCCEEEE
IVHADPAQPHSYVRAMKRLDALIHRLRQGGMDYPHSHISEVINEEDFHSSLTREQYHAVV
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHH
RKAQEYVRSGDIFQVVPSQRLCTQFHARPIDVYRALRTLNPSPYMYFIDIGATQVVGSSP
HHHHHHHHCCCEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCH
EILARLQGDVVTVRPIAGTRPRGATPEHDKRLEMELLADPKERAEHVMLIDLGRNDIGRV
HHHHHHCCCEEEEEECCCCCCCCCCCCCHHCCHHHHHCCCHHHCCEEEEEECCCCHHHHH
VEAGSVQVKESFVIERYSHVMHIVSEVTGILKPGLNYVDVLRATFPAGTVSGAPKIRALE
HHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHH
IIRELEPVCRNVYSGAVGYIGWHGDADTAIAIRTAVIQNGCLYVQAGGGVVYDSDPDLEW
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCEEEECCCCCCCH
QETMNKARALFRAVAQAARGL
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSVSGRKVALITAMQFQRQVAEGCTRIPVVREVQSDLDTPLSIYLKFADAPYTYLFESV
CCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHC
EGGEHFARYSIIGLPARRVYTFHGHTLVVSEFGKVLETRQVADPLAEVELLRAQYVVPQL
CCCCHHHHEEEEECCHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLPGFTGGLVGWFGFECIQYIEPRFMGGKKRDELGTPDILLMLSEEFAVFDSLKGRLYL
CCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEEEECHHHHHHHCCCCEEEE
IVHADPAQPHSYVRAMKRLDALIHRLRQGGMDYPHSHISEVINEEDFHSSLTREQYHAVV
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHH
RKAQEYVRSGDIFQVVPSQRLCTQFHARPIDVYRALRTLNPSPYMYFIDIGATQVVGSSP
HHHHHHHHCCCEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCH
EILARLQGDVVTVRPIAGTRPRGATPEHDKRLEMELLADPKERAEHVMLIDLGRNDIGRV
HHHHHHCCCEEEEEECCCCCCCCCCCCCHHCCHHHHHCCCHHHCCEEEEEECCCCHHHHH
VEAGSVQVKESFVIERYSHVMHIVSEVTGILKPGLNYVDVLRATFPAGTVSGAPKIRALE
HHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHH
IIRELEPVCRNVYSGAVGYIGWHGDADTAIAIRTAVIQNGCLYVQAGGGVVYDSDPDLEW
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCEEEECCCCCCCH
QETMNKARALFRAVAQAARGL
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987141 [H]