Click here to switch to the map view.

The map label for this gene is nudE

Identifier: 182680712

GI number: 182680712

Start: 196087

End: 196635

Strand: Reverse

Name: nudE

Synonym: XfasM23_0141

Alternate gene names: 182680712

Gene position: 196635-196087 (Counterclockwise)

Preceding gene: 182680718

Following gene: 182680711

Centisome position: 7.75

GC content: 53.92

Gene sequence:

>549_bases
ATGAACCGCCCTCTGCCGATGATCCATCGCATTGAGGAAATCGGTGATAGCCAATTTCGGCGGCAGCAGCTCGACCTGGA
GTTTTCCAACGGCGAACGCCGCCTGTACCAACGCCACGTTGCACGAGGATACGGAGCAGTAGTGGTGGTGCCACTACTGG
ATCCGCAGACTGTCCTGCTAGTACGTGAATATGCTGCTGGCATTCACCGTTATGAGCTGGGTCTTGTCAAGGGACGTATT
GATGCCAACGAAACACCGGAGCAGGCCGCTGATCGTGAATTGAAGGAGGAGGCAGGTTACGGTGCGCGGGATCTACGTGT
ACTACGTGCCATCACCCTTGTCCCAGGGTATATGAATCACCAGTCCTGGTTAGTGATAGCATCTGACCTGTACCCAGAAC
GTCTAGTCGGCGACGAACCAGAAGAGATTGAGGTCATTCCTTGGAAGCTTTCAAAGTTGCACGATTTGATGCTGCGTGAG
GATTTCTCCGAAGGGCGCTCCCTGGCAGCATTGTTCATTGTCCGTGAATGGCTGGAACATCAGCAGTGA

Upstream 100 bases:

>100_bases
GCCCGCTGAAGATCGCATGGATCTGCTAGGATTTCAGATTGATGAACATTGTGATGCATCAACTCATTGTATATGCCACT
CCTGCCGGTGTGTGTCTGGC

Downstream 100 bases:

>100_bases
TCACTATTCCCAGCGATCTTCACGAATCGGTCATCGCCATTGCTACAGATTCCGGCAGAGCGATCATGAAGATCTATCAG
GATCATTTCAAGGTCCAGAT

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNRPLPMIHRIEEIGDSQFRRQQLDLEFSNGERRLYQRHVARGYGAVVVVPLLDPQTVLLVREYAAGIHRYELGLVKGRI
DANETPEQAADRELKEEAGYGARDLRVLRAITLVPGYMNHQSWLVIASDLYPERLVGDEPEEIEVIPWKLSKLHDLMLRE
DFSEGRSLAALFIVREWLEHQQ

Sequences:

>Translated_182_residues
MNRPLPMIHRIEEIGDSQFRRQQLDLEFSNGERRLYQRHVARGYGAVVVVPLLDPQTVLLVREYAAGIHRYELGLVKGRI
DANETPEQAADRELKEEAGYGARDLRVLRAITLVPGYMNHQSWLVIASDLYPERLVGDEPEEIEVIPWKLSKLHDLMLRE
DFSEGRSLAALFIVREWLEHQQ
>Mature_182_residues
MNRPLPMIHRIEEIGDSQFRRQQLDLEFSNGERRLYQRHVARGYGAVVVVPLLDPQTVLLVREYAAGIHRYELGLVKGRI
DANETPEQAADRELKEEAGYGARDLRVLRAITLVPGYMNHQSWLVIASDLYPERLVGDEPEEIEVIPWKLSKLHDLMLRE
DFSEGRSLAALFIVREWLEHQQ

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=177, Percent_Identity=46.3276836158192, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21109; Mature: 21109

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPLPMIHRIEEIGDSQFRRQQLDLEFSNGERRLYQRHVARGYGAVVVVPLLDPQTVLL
CCCCCHHHHHHHHHCHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCCHHHH
VREYAAGIHRYELGLVKGRIDANETPEQAADRELKEEAGYGARDLRVLRAITLVPGYMNH
HHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
QSWLVIASDLYPERLVGDEPEEIEVIPWKLSKLHDLMLREDFSEGRSLAALFIVREWLEH
CCEEEEECCCCCHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QQ
CC
>Mature Secondary Structure
MNRPLPMIHRIEEIGDSQFRRQQLDLEFSNGERRLYQRHVARGYGAVVVVPLLDPQTVLL
CCCCCHHHHHHHHHCHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCCHHHH
VREYAAGIHRYELGLVKGRIDANETPEQAADRELKEEAGYGARDLRVLRAITLVPGYMNH
HHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
QSWLVIASDLYPERLVGDEPEEIEVIPWKLSKLHDLMLREDFSEGRSLAALFIVREWLEH
CCEEEEECCCCCHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]