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The map label for this gene is bioB

Identifier: 182680613

GI number: 182680613

Start: 57489

End: 58514

Strand: Direct

Name: bioB

Synonym: XfasM23_0041

Alternate gene names: 182680613

Gene position: 57489-58514 (Clockwise)

Preceding gene: 182680610

Following gene: 182680614

Centisome position: 2.27

GC content: 51.36

Gene sequence:

>1026_bases
ATGTCCTCTATCATCCGCTACGACTGGACTGCCGAAGAGTTACATGCGCTGTTCGATTTGTCGTTGCCAGAACTTCTTTA
TCGGGCCGCTAGTGTGCATCGTCAGCATTTCGACCCTGCTGAGATTCAGGTGTCTACATTGTTGTCGGTTAAGACTGGCG
GCTGTCCGGAAGATTGTTCTTATTGTCCACAGGCGCAGCGTTATGACACTGGTGTGACAGCGCAGAAGTTGATGGACGTT
GATGCAGTCGTTGCGAAGGCACGACAAGCGAAGTTGGCCGGTGCGTCCCGTTTTTGCATGGGGGCCGCTTGGCGCTCACC
CAAGGATCGGGATATTCCAAAAATTGCGTCGATGATTCGGGAGGTTAAAGCACTTGGCCTGGAAACGTGTGCCACGCTAG
GCATGCTCAATACGTGTCAGGCGCAGGCGCTCAAGGATGCTGGTCTGGATTACTACAATCATAATGTGGATACATCGCCG
GATTTTTACGATTCGGTCATCCACACTCGGCAATATCAGGATCGGCTGGATACTTTGGCCCATGTGCGTGATGTTGGTTT
GAAAACGTGTTGTGGTGGCATTGTCGGCATGGGCGAGACGCGTCAGCATCGCGTTGGTTTGCTGCTGACTCTAGCTACTC
TGCCAGCGCATCCTGATTCTGTGCCGGTCAATCTTTTGGTTCAAGTAGCTGGGACTCCTCTGCATGGTACACAGACGCTT
GATCCGTTTGAGTTTGTACGCATGATTGCAGTGGCACGGATCACTATGCCCAGGTCGATGGTACGTCTTTCCGCAGGGCG
GGAGTCGATGAGTGATGAGTTACAGTTGCTTTGCTTTATGGCTGGTGCCAACTCGATTTTCTACGGTGAGAAACTGTTGA
CCACTGCTAATCCGGAGACTGAACGGGATCAAGCTCTGTTCCAGCGCCTGGGATTGCGTCCAATGCATCTCATGGAGAAT
GTTTCTAACAAAGATCAGCATCACGGTAATGTACATGCTGATATCGCTTGCAAACATGTTGTTTGA

Upstream 100 bases:

>100_bases
ATCACACCAAGTGTCAGTCAATATGTGATTCGAGTTGACAGCGTCTTTCATCCTGACCAGACTGCCGAACTTGTTGATCG
GTTACCGTCAGGAAACGTTG

Downstream 100 bases:

>100_bases
AGTGATTTGTTGTCTATGTACAAAAACCCCACCCTATAGGATGGGGCGTCTTATTGGCGACATTGGTGGATGTTGTGTCG
TTCATGTATGACGGTGTCAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSSIIRYDWTAEELHALFDLSLPELLYRAASVHRQHFDPAEIQVSTLLSVKTGGCPEDCSYCPQAQRYDTGVTAQKLMDV
DAVVAKARQAKLAGASRFCMGAAWRSPKDRDIPKIASMIREVKALGLETCATLGMLNTCQAQALKDAGLDYYNHNVDTSP
DFYDSVIHTRQYQDRLDTLAHVRDVGLKTCCGGIVGMGETRQHRVGLLLTLATLPAHPDSVPVNLLVQVAGTPLHGTQTL
DPFEFVRMIAVARITMPRSMVRLSAGRESMSDELQLLCFMAGANSIFYGEKLLTTANPETERDQALFQRLGLRPMHLMEN
VSNKDQHHGNVHADIACKHVV

Sequences:

>Translated_341_residues
MSSIIRYDWTAEELHALFDLSLPELLYRAASVHRQHFDPAEIQVSTLLSVKTGGCPEDCSYCPQAQRYDTGVTAQKLMDV
DAVVAKARQAKLAGASRFCMGAAWRSPKDRDIPKIASMIREVKALGLETCATLGMLNTCQAQALKDAGLDYYNHNVDTSP
DFYDSVIHTRQYQDRLDTLAHVRDVGLKTCCGGIVGMGETRQHRVGLLLTLATLPAHPDSVPVNLLVQVAGTPLHGTQTL
DPFEFVRMIAVARITMPRSMVRLSAGRESMSDELQLLCFMAGANSIFYGEKLLTTANPETERDQALFQRLGLRPMHLMEN
VSNKDQHHGNVHADIACKHVV
>Mature_340_residues
SSIIRYDWTAEELHALFDLSLPELLYRAASVHRQHFDPAEIQVSTLLSVKTGGCPEDCSYCPQAQRYDTGVTAQKLMDVD
AVVAKARQAKLAGASRFCMGAAWRSPKDRDIPKIASMIREVKALGLETCATLGMLNTCQAQALKDAGLDYYNHNVDTSPD
FYDSVIHTRQYQDRLDTLAHVRDVGLKTCCGGIVGMGETRQHRVGLLLTLATLPAHPDSVPVNLLVQVAGTPLHGTQTLD
PFEFVRMIAVARITMPRSMVRLSAGRESMSDELQLLCFMAGANSIFYGEKLLTTANPETERDQALFQRLGLRPMHLMENV
SNKDQHHGNVHADIACKHVV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=323, Percent_Identity=58.8235294117647, Blast_Score=416, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=45.4545454545455, Blast_Score=313, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_XYLF2 (B2I672)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828773.1
- ProteinModelPortal:   B2I672
- SMR:   B2I672
- GeneID:   6202038
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0041
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2300892
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37691; Mature: 37560

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIIRYDWTAEELHALFDLSLPELLYRAASVHRQHFDPAEIQVSTLLSVKTGGCPEDCS
CCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEHHHHEEECCCCCCHHHH
YCPQAQRYDTGVTAQKLMDVDAVVAKARQAKLAGASRFCMGAAWRSPKDRDIPKIASMIR
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHH
EVKALGLETCATLGMLNTCQAQALKDAGLDYYNHNVDTSPDFYDSVIHTRQYQDRLDTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETRQHRVGLLLTLATLPAHPDSVPVNLLVQVAGTPLHGTQTL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEHHCCCCCCCCCHHHEEEECCCCCCCCCCC
DPFEFVRMIAVARITMPRSMVRLSAGRESMSDELQLLCFMAGANSIFYGEKLLTTANPET
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCCC
ERDQALFQRLGLRPMHLMENVSNKDQHHGNVHADIACKHVV
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEECEEEECCC
>Mature Secondary Structure 
SSIIRYDWTAEELHALFDLSLPELLYRAASVHRQHFDPAEIQVSTLLSVKTGGCPEDCS
CCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEHHHHEEECCCCCCHHHH
YCPQAQRYDTGVTAQKLMDVDAVVAKARQAKLAGASRFCMGAAWRSPKDRDIPKIASMIR
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHH
EVKALGLETCATLGMLNTCQAQALKDAGLDYYNHNVDTSPDFYDSVIHTRQYQDRLDTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETRQHRVGLLLTLATLPAHPDSVPVNLLVQVAGTPLHGTQTL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEHHCCCCCCCCCHHHEEEECCCCCCCCCCC
DPFEFVRMIAVARITMPRSMVRLSAGRESMSDELQLLCFMAGANSIFYGEKLLTTANPET
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCCC
ERDQALFQRLGLRPMHLMENVSNKDQHHGNVHADIACKHVV
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEECEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA