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The map label for this gene is pdxJ

Identifier: 182680610

GI number: 182680610

Start: 53289

End: 54071

Strand: Direct

Name: pdxJ

Synonym: XfasM23_0038

Alternate gene names: 182680610

Gene position: 53289-54071 (Clockwise)

Preceding gene: 182680609

Following gene: 182680613

Centisome position: 2.1

GC content: 51.47

Gene sequence:

>783_bases
ATGAGTCAGCGTACTCGGTTGAGTGTCAACGTTAATAAGATTGCAGTTTTACGTAACTCCCGTGGTCACGGTGCACCCGA
TGTGATACGTGCAGCTAGTGCCTGCATTGATGCAGGTGCGCACGGTATTACGGTGCATCCGCGTCCTGATGCACGTCATA
TCCGCCATGATGATGTGATCAGGTTATCGGCTTTGACCCGTGCACGCGGTGTGGAATTCAACATCGAAGGTAATCCGTTT
GCAGAGCCACGTGCAGGTTATTGTGGGCTTCTGGCACTTTGCCGTGAAACCCGCCCTCATCAGGTCACTTTGGTTCCCGA
TGGGGATCAGCAGATAACATCCGATCACGGTTTCGATTTTGCACGGGAAGGTCCTGGGCTGCGTCCATTGATTGATGAAA
TCAAGCAGTGGGGATGTCGCGTGAGTCTATTTGTTGACGTTAACGTCACCGGTCTGGCTGATGCAGCAATATGGGGTGTG
GATCGGATTGAACTTTATACGGGGCCGTATGCGGAGATGCATCATGCGGGTTGTTCGGATGCTGTGTTGCGTGAATTTGC
TACTACGGCACGTCTTGCGCAGGATGTCGGCTTGGGTGTCAATGCTGGCCATGACTTGTCGCAAACGAATCTTGGTGTGT
TTTTAGGGGCTGTCCCTGATGTGTTGGAGGTTTCGATTGGTCACGCACTGATCAGTGAGGCGTTATATGAGGGATTAATA
CCAACAGTGCGCCGTTACTTGGATATTCTGGATTCGGTCAACCCTGCTGTTTCTATGCGTTGA

Upstream 100 bases:

>100_bases
TGGTTGATGAAGTTGAATTACAGAGGTTGCAGGAATTGGCTGCAGTCCGCAGGTTATCTCCTGTTGTTGGGGGTAATGAT
GATGTCGGTGGAATAAATAA

Downstream 100 bases:

>100_bases
TGTTGTTTCGGAGTCTGTGAGGTTTATAGGATTTTGTGGTGACCTCAAGGCATCTGAGTAGGTGTGACGCTACAGGCGGG
TGTTCATCATGATGTGTTGT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSQRTRLSVNVNKIAVLRNSRGHGAPDVIRAASACIDAGAHGITVHPRPDARHIRHDDVIRLSALTRARGVEFNIEGNPF
AEPRAGYCGLLALCRETRPHQVTLVPDGDQQITSDHGFDFAREGPGLRPLIDEIKQWGCRVSLFVDVNVTGLADAAIWGV
DRIELYTGPYAEMHHAGCSDAVLREFATTARLAQDVGLGVNAGHDLSQTNLGVFLGAVPDVLEVSIGHALISEALYEGLI
PTVRRYLDILDSVNPAVSMR

Sequences:

>Translated_260_residues
MSQRTRLSVNVNKIAVLRNSRGHGAPDVIRAASACIDAGAHGITVHPRPDARHIRHDDVIRLSALTRARGVEFNIEGNPF
AEPRAGYCGLLALCRETRPHQVTLVPDGDQQITSDHGFDFAREGPGLRPLIDEIKQWGCRVSLFVDVNVTGLADAAIWGV
DRIELYTGPYAEMHHAGCSDAVLREFATTARLAQDVGLGVNAGHDLSQTNLGVFLGAVPDVLEVSIGHALISEALYEGLI
PTVRRYLDILDSVNPAVSMR
>Mature_259_residues
SQRTRLSVNVNKIAVLRNSRGHGAPDVIRAASACIDAGAHGITVHPRPDARHIRHDDVIRLSALTRARGVEFNIEGNPFA
EPRAGYCGLLALCRETRPHQVTLVPDGDQQITSDHGFDFAREGPGLRPLIDEIKQWGCRVSLFVDVNVTGLADAAIWGVD
RIELYTGPYAEMHHAGCSDAVLREFATTARLAQDVGLGVNAGHDLSQTNLGVFLGAVPDVLEVSIGHALISEALYEGLIP
TVRRYLDILDSVNPAVSMR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=239, Percent_Identity=37.6569037656904, Blast_Score=134, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_XYLF2 (B2I669)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828770.1
- ProteinModelPortal:   B2I669
- SMR:   B2I669
- GeneID:   6202021
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0038
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 28149; Mature: 28018

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: ACT_SITE 46-46 ACT_SITE 76-76 ACT_SITE 204-204 BINDING 10-10 BINDING 21-21 BINDING 48-48 BINDING 53-53 BINDING 113-113 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRTRLSVNVNKIAVLRNSRGHGAPDVIRAASACIDAGAHGITVHPRPDARHIRHDDVI
CCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEE
RLSALTRARGVEFNIEGNPFAEPRAGYCGLLALCRETRPHQVTLVPDGDQQITSDHGFDF
EHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCH
AREGPGLRPLIDEIKQWGCRVSLFVDVNVTGLADAAIWGVDRIELYTGPYAEMHHAGCSD
HHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHCCCEEEEEECCHHHHHHCCCHH
AVLREFATTARLAQDVGLGVNAGHDLSQTNLGVFLGAVPDVLEVSIGHALISEALYEGLI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
PTVRRYLDILDSVNPAVSMR
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQRTRLSVNVNKIAVLRNSRGHGAPDVIRAASACIDAGAHGITVHPRPDARHIRHDDVI
CCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEE
RLSALTRARGVEFNIEGNPFAEPRAGYCGLLALCRETRPHQVTLVPDGDQQITSDHGFDF
EHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCH
AREGPGLRPLIDEIKQWGCRVSLFVDVNVTGLADAAIWGVDRIELYTGPYAEMHHAGCSD
HHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHCCCEEEEEECCHHHHHHCCCHH
AVLREFATTARLAQDVGLGVNAGHDLSQTNLGVFLGAVPDVLEVSIGHALISEALYEGLI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
PTVRRYLDILDSVNPAVSMR
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA