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The map label for this gene is mscL

Identifier: 182680599

GI number: 182680599

Start: 34813

End: 35217

Strand: Reverse

Name: mscL

Synonym: XfasM23_0027

Alternate gene names: 182680599

Gene position: 35217-34813 (Counterclockwise)

Preceding gene: 182680607

Following gene: 182680597

Centisome position: 1.39

GC content: 45.43

Gene sequence:

>405_bases
GTGAGTTTTATCCGCGAATTTAAAGAATTTGTAATGCGTGGCAACGTCATTGACCTCGCCGTAGCAGTGGTGATTGGTGC
AGCATTCGGAAAAATCGTTACCGCACTGGTTGATAAAATTATTTCACCACTCATCGGTGTGATGGTCGGAGGGATCGACT
TCTCCAAGCTCTCCCTCACCTTGAAAGCTGCGACAGTGGATACAGCAGGGAAGGAGGTGCCAGCCGTCGTGATTGGCTAC
GGTGACTTCATCAACACGATCCTACAATTCATCATCATTGCTTTCGCAATTTTCATCATCGTCAAAATGATTAATAAGGT
GATTAACAAGCAACCCTTACCACCAGAAACACCTAGCGAAGATGTGCTACTACTACGCGAGATTCGCGATTCGCTGAAAA
AATAA

Upstream 100 bases:

>100_bases
CAAGCCTAGACAGAGAACAACATAAATCCTGCTTTGATCGACAACCCGCTACGATGCACCACATGCGGCGATACGACCGC
GAGGAGATAAGGAGTTAAAT

Downstream 100 bases:

>100_bases
TTCTGCTGGCATACGAAAAAACATCGCTTCTGCTGCCGTAGTGTCGCAAAGCCACGCAATCCGTAAAACGTATGAATCGC
TGTAAAAAATAATGTTCTGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MSFIREFKEFVMRGNVIDLAVAVVIGAAFGKIVTALVDKIISPLIGVMVGGIDFSKLSLTLKAATVDTAGKEVPAVVIGY
GDFINTILQFIIIAFAIFIIVKMINKVINKQPLPPETPSEDVLLLREIRDSLKK

Sequences:

>Translated_134_residues
MSFIREFKEFVMRGNVIDLAVAVVIGAAFGKIVTALVDKIISPLIGVMVGGIDFSKLSLTLKAATVDTAGKEVPAVVIGY
GDFINTILQFIIIAFAIFIIVKMINKVINKQPLPPETPSEDVLLLREIRDSLKK
>Mature_133_residues
SFIREFKEFVMRGNVIDLAVAVVIGAAFGKIVTALVDKIISPLIGVMVGGIDFSKLSLTLKAATVDTAGKEVPAVVIGYG
DFINTILQFIIIAFAIFIIVKMINKVINKQPLPPETPSEDVLLLREIRDSLKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=52.2058823529412, Blast_Score=136, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_XYLF2 (B2I658)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828759.1
- ProteinModelPortal:   B2I658
- SMR:   B2I658
- GeneID:   6201924
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0027
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14573; Mature: 14442

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x112cbd10)-; HASH(0x12fda98c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIREFKEFVMRGNVIDLAVAVVIGAAFGKIVTALVDKIISPLIGVMVGGIDFSKLSLT
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKAATVDTAGKEVPAVVIGYGDFINTILQFIIIAFAIFIIVKMINKVINKQPLPPETPSE
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
DVLLLREIRDSLKK
HHHHHHHHHHHHCH
>Mature Secondary Structure 
SFIREFKEFVMRGNVIDLAVAVVIGAAFGKIVTALVDKIISPLIGVMVGGIDFSKLSLT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKAATVDTAGKEVPAVVIGYGDFINTILQFIIIAFAIFIIVKMINKVINKQPLPPETPSE
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
DVLLLREIRDSLKK
HHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA