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The map label for this gene is pyrF

Identifier: 182680597

GI number: 182680597

Start: 30869

End: 31603

Strand: Reverse

Name: pyrF

Synonym: XfasM23_0025

Alternate gene names: 182680597

Gene position: 31603-30869 (Counterclockwise)

Preceding gene: 182680599

Following gene: 182680589

Centisome position: 1.25

GC content: 53.61

Gene sequence:

>735_bases
ATGATGAATCACACGCCGTTATTACTGGGGATCCGCGAGCGGTTGATCTTCGCACTAGACGTACCCAGTCGAACGCAAGC
ATTGGAATGGATCGACCAATTGGGTGATGCCATCTCCTTCTATAAGATCGGCATGGAATTGTTGGCTTCCGGCGAATATT
TCCAGGTACTCGACGACTTGGCTTCGCGTGGCAAACGTGTCTTCGTCGACCTAAAATTCTTCGACATCCCCGCTACAGTC
GCAGGAGTCATCCGTCGTCTTTCGCAGTGGCCCATCAGCTATTGCACAATCCATGGCTGGCACGCGCCCATGATGCAAGC
GGCGACCGAAGCCAATACGAGCAACATGCATTTACTGGCAGTTACCGTGCTGACATCGATGACACGTGAAGATTTAGCGA
AGATGGGTATCAACCGCGAACCCGTTGATGTCGTGGTTGAACGTGCATTAGCCGCCCACATGGCGGGGATGAGCGGTGTG
ATTGCTTCTGGACAGGAAGCAGCAGCAATCCGCCACGCCATCGGCTCTGGTTTCTCAATCGTTTGCCCAGGCATTCGCAC
AAACCATGTGCCTCACAATGACCAACAACGCACGATAGGCATCAAGGCTGCTTTTGCCAACGGTGCAGATGCGATTGTGG
TGGGACGCCCAATCCGAATGGCGCAGGATCCGCAAGCGGCAGCAGAGGCTATGCAAACAGAAATCATGACGGCACTCACT
GAGCCATCAACATAA

Upstream 100 bases:

>100_bases
CGTTAATAACGACAATTGACAGATAGCCTGGTTCCACACCAACAGCAACCGGGCACGATCGGTTTTATGATGCAGAGAAA
CATTTCACATTGGATCGACC

Downstream 100 bases:

>100_bases
AATCTGACGCCATGTCCTACGCAAACGTGTTGCCATACAGGCCTACGGTCTTAAAGAAGCAACACGTTCACCACATGATG
TTAGCAATCGCTTTTGTTCA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMNHTPLLLGIRERLIFALDVPSRTQALEWIDQLGDAISFYKIGMELLASGEYFQVLDDLASRGKRVFVDLKFFDIPATV
AGVIRRLSQWPISYCTIHGWHAPMMQAATEANTSNMHLLAVTVLTSMTREDLAKMGINREPVDVVVERALAAHMAGMSGV
IASGQEAAAIRHAIGSGFSIVCPGIRTNHVPHNDQQRTIGIKAAFANGADAIVVGRPIRMAQDPQAAAEAMQTEIMTALT
EPST

Sequences:

>Translated_244_residues
MMNHTPLLLGIRERLIFALDVPSRTQALEWIDQLGDAISFYKIGMELLASGEYFQVLDDLASRGKRVFVDLKFFDIPATV
AGVIRRLSQWPISYCTIHGWHAPMMQAATEANTSNMHLLAVTVLTSMTREDLAKMGINREPVDVVVERALAAHMAGMSGV
IASGQEAAAIRHAIGSGFSIVCPGIRTNHVPHNDQQRTIGIKAAFANGADAIVVGRPIRMAQDPQAAAEAMQTEIMTALT
EPST
>Mature_244_residues
MMNHTPLLLGIRERLIFALDVPSRTQALEWIDQLGDAISFYKIGMELLASGEYFQVLDDLASRGKRVFVDLKFFDIPATV
AGVIRRLSQWPISYCTIHGWHAPMMQAATEANTSNMHLLAVTVLTSMTREDLAKMGINREPVDVVVERALAAHMAGMSGV
IASGQEAAAIRHAIGSGFSIVCPGIRTNHVPHNDQQRTIGIKAAFANGADAIVVGRPIRMAQDPQAAAEAMQTEIMTALT
EPST

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=32.8947368421053, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_XYLF2 (B2I656)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828757.1
- ProteinModelPortal:   B2I656
- GeneID:   6201919
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0025
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26506; Mature: 26506

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 72-72 BINDING 20-20 BINDING 42-42 BINDING 125-125 BINDING 186-186 BINDING 195-195 BINDING 215-215 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNHTPLLLGIRERLIFALDVPSRTQALEWIDQLGDAISFYKIGMELLASGEYFQVLDDL
CCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ASRGKRVFVDLKFFDIPATVAGVIRRLSQWPISYCTIHGWHAPMMQAATEANTSNMHLLA
HHCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHH
VTVLTSMTREDLAKMGINREPVDVVVERALAAHMAGMSGVIASGQEAAAIRHAIGSGFSI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCEE
VCPGIRTNHVPHNDQQRTIGIKAAFANGADAIVVGRPIRMAQDPQAAAEAMQTEIMTALT
EECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
EPST
CCCC
>Mature Secondary Structure
MMNHTPLLLGIRERLIFALDVPSRTQALEWIDQLGDAISFYKIGMELLASGEYFQVLDDL
CCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ASRGKRVFVDLKFFDIPATVAGVIRRLSQWPISYCTIHGWHAPMMQAATEANTSNMHLLA
HHCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHH
VTVLTSMTREDLAKMGINREPVDVVVERALAAHMAGMSGVIASGQEAAAIRHAIGSGFSI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCEE
VCPGIRTNHVPHNDQQRTIGIKAAFANGADAIVVGRPIRMAQDPQAAAEAMQTEIMTALT
EECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
EPST
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA