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The map label for this gene is uvrD

Identifier: 182680607

GI number: 182680607

Start: 46520

End: 48691

Strand: Reverse

Name: uvrD

Synonym: XfasM23_0035

Alternate gene names: 182680607

Gene position: 48691-46520 (Counterclockwise)

Preceding gene: 182680611

Following gene: 182680599

Centisome position: 1.92

GC content: 54.93

Gene sequence:

>2172_bases
GTGCTTGATCACTTAAATCAGAACCAGCGCGAGGCCGTTGCTGCAGCTCCCGGCCACTACCTGATCCTCGCCGGTGCCGG
ATCAGGAAAAACCCGCGTTCTCACTCATCGCATCGTTTGGCTCAACGAGGTAAATGGGGTACCCACCCACAGCATGATGG
CCGTCACCTTCACGAACAAGGCCGCCGGCGAAATACAACAACGGATTGATCTACAACTGCGCCACGGCAAACGCGGCATG
TGGATCGGGACCTTCCATAGCCTGGCGCATCGCTTACTGCGCCTGCATTGGAACGACGCAACATTACCCGAAAACTTCCA
GGTCATAGACAGCGACGATCAATTGCGCCTCGTCAAACGAGTGATCCAGTCCTTGGGATTGAATGACACCCTCTTCCCTC
CCAAACAGGCGATGTGGTGGATTAATACACAGAAAGATGAAGGACGCCGCCCAGAGCACATCCAACCCGAGTCACACGAC
GACTGGATCGAGACGCAACGCCGCATCTATACCGAGTATCAGGAGCGCTGCAACCGCGCTGGTTTGGTCGATTTTTCGGA
ACTGCTGCTGCGGGCACATGAACTACTACGCGATACACCTGCACTACTGACACACTACCGTTCGCGCTTCCGTGAGATTC
TGATCGACGAATTCCAAGATACCAACGCCATCCAGTACGCGTTTGTGCGTGTCCTCGCCAATGACACTGGCCACATCTTC
GCCGTCGGCGATGACGACCAAGCCATCTACGGCTGGCGTGGTGCCAAAGTTGAGCATGTACAACACTTCCTGAAAGATTT
TCCAAACGTGCAGACCATCCGCTTGGAGCAAAATTACCGCTCCAGCGCCAATATCCTTGACGCTGCCAACGCAATCATTG
CCCATAACCCAGACCGAATTGGCAAACGCCTCTGGACTGAATCCGGTGTTGGTGAATCCATCGACCTATACGCCGCCTAC
AACGAGATTGATGAAGCCCGCTACATCGTCGAACGCGCCCGCCAGTGGGTACGCGACGGCGGCAGCTACGGCGAGATCGC
AGTGCTTTACCGTAGTAACGCACAGTCCCGCGCGTTTGAAGAAGTGCTGCAATCCGAACATATCCCCTACCGTGTTTACG
GCGGCATGCGCTTCTTCGAGCGTGCCGAAATCAAGGATGCCCTAGCCTATCTGCGCCTGCTTGCCAATCGTAGCGATGAT
GCTGCGTTCGAACGCGTTGTAAATACCCCCGTACGCGGCATCGGTGAGCGCACCTTAGATGAAGTAAGACACCTAGCACG
TACCCAAGGCTTAGCACTATGGGATGCGGCAATGGCATGTACCCAATCAACCACGCTGACCGCGCGCGCGCGTAACGCCT
TGGCGAGTTTCCTCAATTTACTGCAACAACTTGCGGTGGAAACCAACCAGATGGGTTTAGCCGAACGAATTGACCATATG
CTGCAGCGTTCGGAGTTACGCCAGCACTGGAAGAAAGAAGCGCGTGGTTCTCTGGATGCCGAATCACGTCAAGAAAACTT
AGACGAACTTGTTTCCGTCGCTTCACGCTTTGTCCTTCCGCAAAACGACGAAGACACACCCATCATGACCGAATTAGACG
CGTTCCTATCCTACGCGTGCCTGGAAAGTGGTGATGGTCAAGTCCAAGCTGGTGAAGAAGGCGTACAACTGATGACCCTA
CACTCAGCCAAAGGCTTGGAATTCCCCCTAGTCTTCTTGGTGGGCTTGGAAAATGGATTATTCCCCAGCGCGCGTTCCCT
GGATGAACACGGCCGACTAGAGGAAGAACGCCGCTTAGCGTATGTCGGCATCACCCGCGCCCGTCACAAACTCATCCTCA
GCTATGCCCAAGCACGGCGTATCCACGGTAAAGATAACTACAACGTCCCCTCACGCTTTTTACGCGAGATCCCGCAGGAT
CTATTGCATGAAGTACGCCCCACCATCCAGGTCTCACACCAGCCCCCATTAGGAGCAACGCAACGTCCAGCCCATGATGA
ACGCGACGCAGCACCTATCAAGCTCGGCGTGAACGTCATACACCCTACATTTGGGTGCGGCGTGGTTGTCGACTACGAAG
GCAGCGGCGTGCACACCCGCGTACAAATCAAATTCGATGACGCCGGTACAAAATGGTTGGTCATGGCATACGCCAAACTC
AGCGTGGTATAG

Upstream 100 bases:

>100_bases
GCTCCAGCATGACTCTCCTCAATGCACCCCCGAATGCCCAGACACTTAGAATGAATATCATCCTGCCACCACGTAGAATC
ATTGCGTGAACGTCTCTCAT

Downstream 100 bases:

>100_bases
ACACACATCACCACTGCACTGTGCCTCTCGCACTCTCACTGCAACAGTGCATACCGACGTCATAGACTTTGTCTCCCCCG
ACATCAATACGATGCCGGGG

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MLDHLNQNQREAVAAAPGHYLILAGAGSGKTRVLTHRIVWLNEVNGVPTHSMMAVTFTNKAAGEIQQRIDLQLRHGKRGM
WIGTFHSLAHRLLRLHWNDATLPENFQVIDSDDQLRLVKRVIQSLGLNDTLFPPKQAMWWINTQKDEGRRPEHIQPESHD
DWIETQRRIYTEYQERCNRAGLVDFSELLLRAHELLRDTPALLTHYRSRFREILIDEFQDTNAIQYAFVRVLANDTGHIF
AVGDDDQAIYGWRGAKVEHVQHFLKDFPNVQTIRLEQNYRSSANILDAANAIIAHNPDRIGKRLWTESGVGESIDLYAAY
NEIDEARYIVERARQWVRDGGSYGEIAVLYRSNAQSRAFEEVLQSEHIPYRVYGGMRFFERAEIKDALAYLRLLANRSDD
AAFERVVNTPVRGIGERTLDEVRHLARTQGLALWDAAMACTQSTTLTARARNALASFLNLLQQLAVETNQMGLAERIDHM
LQRSELRQHWKKEARGSLDAESRQENLDELVSVASRFVLPQNDEDTPIMTELDAFLSYACLESGDGQVQAGEEGVQLMTL
HSAKGLEFPLVFLVGLENGLFPSARSLDEHGRLEEERRLAYVGITRARHKLILSYAQARRIHGKDNYNVPSRFLREIPQD
LLHEVRPTIQVSHQPPLGATQRPAHDERDAAPIKLGVNVIHPTFGCGVVVDYEGSGVHTRVQIKFDDAGTKWLVMAYAKL
SVV

Sequences:

>Translated_723_residues
MLDHLNQNQREAVAAAPGHYLILAGAGSGKTRVLTHRIVWLNEVNGVPTHSMMAVTFTNKAAGEIQQRIDLQLRHGKRGM
WIGTFHSLAHRLLRLHWNDATLPENFQVIDSDDQLRLVKRVIQSLGLNDTLFPPKQAMWWINTQKDEGRRPEHIQPESHD
DWIETQRRIYTEYQERCNRAGLVDFSELLLRAHELLRDTPALLTHYRSRFREILIDEFQDTNAIQYAFVRVLANDTGHIF
AVGDDDQAIYGWRGAKVEHVQHFLKDFPNVQTIRLEQNYRSSANILDAANAIIAHNPDRIGKRLWTESGVGESIDLYAAY
NEIDEARYIVERARQWVRDGGSYGEIAVLYRSNAQSRAFEEVLQSEHIPYRVYGGMRFFERAEIKDALAYLRLLANRSDD
AAFERVVNTPVRGIGERTLDEVRHLARTQGLALWDAAMACTQSTTLTARARNALASFLNLLQQLAVETNQMGLAERIDHM
LQRSELRQHWKKEARGSLDAESRQENLDELVSVASRFVLPQNDEDTPIMTELDAFLSYACLESGDGQVQAGEEGVQLMTL
HSAKGLEFPLVFLVGLENGLFPSARSLDEHGRLEEERRLAYVGITRARHKLILSYAQARRIHGKDNYNVPSRFLREIPQD
LLHEVRPTIQVSHQPPLGATQRPAHDERDAAPIKLGVNVIHPTFGCGVVVDYEGSGVHTRVQIKFDDAGTKWLVMAYAKL
SVV
>Mature_723_residues
MLDHLNQNQREAVAAAPGHYLILAGAGSGKTRVLTHRIVWLNEVNGVPTHSMMAVTFTNKAAGEIQQRIDLQLRHGKRGM
WIGTFHSLAHRLLRLHWNDATLPENFQVIDSDDQLRLVKRVIQSLGLNDTLFPPKQAMWWINTQKDEGRRPEHIQPESHD
DWIETQRRIYTEYQERCNRAGLVDFSELLLRAHELLRDTPALLTHYRSRFREILIDEFQDTNAIQYAFVRVLANDTGHIF
AVGDDDQAIYGWRGAKVEHVQHFLKDFPNVQTIRLEQNYRSSANILDAANAIIAHNPDRIGKRLWTESGVGESIDLYAAY
NEIDEARYIVERARQWVRDGGSYGEIAVLYRSNAQSRAFEEVLQSEHIPYRVYGGMRFFERAEIKDALAYLRLLANRSDD
AAFERVVNTPVRGIGERTLDEVRHLARTQGLALWDAAMACTQSTTLTARARNALASFLNLLQQLAVETNQMGLAERIDHM
LQRSELRQHWKKEARGSLDAESRQENLDELVSVASRFVLPQNDEDTPIMTELDAFLSYACLESGDGQVQAGEEGVQLMTL
HSAKGLEFPLVFLVGLENGLFPSARSLDEHGRLEEERRLAYVGITRARHKLILSYAQARRIHGKDNYNVPSRFLREIPQD
LLHEVRPTIQVSHQPPLGATQRPAHDERDAAPIKLGVNVIHPTFGCGVVVDYEGSGVHTRVQIKFDDAGTKWLVMAYAKL
SVV

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=731, Percent_Identity=56.2243502051984, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=38.2581648522551, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=124, Percent_Identity=34.6774193548387, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=763, Percent_Identity=25.0327653997379, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6324477, Length=666, Percent_Identity=21.7717717717718, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82233; Mature: 82233

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDHLNQNQREAVAAAPGHYLILAGAGSGKTRVLTHRIVWLNEVNGVPTHSMMAVTFTNK
CCCCCCCCHHHHHHCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCEEEEEECCH
AAGEIQQRIDLQLRHGKRGMWIGTFHSLAHRLLRLHWNDATLPENFQVIDSDDQLRLVKR
HHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHH
VIQSLGLNDTLFPPKQAMWWINTQKDEGRRPEHIQPESHDDWIETQRRIYTEYQERCNRA
HHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GLVDFSELLLRAHELLRDTPALLTHYRSRFREILIDEFQDTNAIQYAFVRVLANDTGHIF
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEE
AVGDDDQAIYGWRGAKVEHVQHFLKDFPNVQTIRLEQNYRSSANILDAANAIIAHNPDRI
EECCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCHHEECCHHHH
GKRLWTESGVGESIDLYAAYNEIDEARYIVERARQWVRDGGSYGEIAVLYRSNAQSRAFE
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
EVLQSEHIPYRVYGGMRFFERAEIKDALAYLRLLANRSDDAAFERVVNTPVRGIGERTLD
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCHHHHH
EVRHLARTQGLALWDAAMACTQSTTLTARARNALASFLNLLQQLAVETNQMGLAERIDHM
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQRSELRQHWKKEARGSLDAESRQENLDELVSVASRFVLPQNDEDTPIMTELDAFLSYAC
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LESGDGQVQAGEEGVQLMTLHSAKGLEFPLVFLVGLENGLFPSARSLDEHGRLEEERRLA
HCCCCCCEEECCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCHHHEEE
YVGITRARHKLILSYAQARRIHGKDNYNVPSRFLREIPQDLLHEVRPTIQVSHQPPLGAT
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
QRPAHDERDAAPIKLGVNVIHPTFGCGVVVDYEGSGVHTRVQIKFDDAGTKWLVMAYAKL
CCCCCCCCCCCCEEECCEEECCCCCCCEEEEECCCCEEEEEEEEECCCCCEEEEEEHHHH
SVV
HCC
>Mature Secondary Structure
MLDHLNQNQREAVAAAPGHYLILAGAGSGKTRVLTHRIVWLNEVNGVPTHSMMAVTFTNK
CCCCCCCCHHHHHHCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCEEEEEECCH
AAGEIQQRIDLQLRHGKRGMWIGTFHSLAHRLLRLHWNDATLPENFQVIDSDDQLRLVKR
HHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHH
VIQSLGLNDTLFPPKQAMWWINTQKDEGRRPEHIQPESHDDWIETQRRIYTEYQERCNRA
HHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GLVDFSELLLRAHELLRDTPALLTHYRSRFREILIDEFQDTNAIQYAFVRVLANDTGHIF
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEE
AVGDDDQAIYGWRGAKVEHVQHFLKDFPNVQTIRLEQNYRSSANILDAANAIIAHNPDRI
EECCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCHHEECCHHHH
GKRLWTESGVGESIDLYAAYNEIDEARYIVERARQWVRDGGSYGEIAVLYRSNAQSRAFE
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
EVLQSEHIPYRVYGGMRFFERAEIKDALAYLRLLANRSDDAAFERVVNTPVRGIGERTLD
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCHHHHH
EVRHLARTQGLALWDAAMACTQSTTLTARARNALASFLNLLQQLAVETNQMGLAERIDHM
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQRSELRQHWKKEARGSLDAESRQENLDELVSVASRFVLPQNDEDTPIMTELDAFLSYAC
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LESGDGQVQAGEEGVQLMTLHSAKGLEFPLVFLVGLENGLFPSARSLDEHGRLEEERRLA
HCCCCCCEEECCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCHHHEEE
YVGITRARHKLILSYAQARRIHGKDNYNVPSRFLREIPQDLLHEVRPTIQVSHQPPLGAT
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
QRPAHDERDAAPIKLGVNVIHPTFGCGVVVDYEGSGVHTRVQIKFDDAGTKWLVMAYAKL
CCCCCCCCCCCCEEECCEEECCCCCCCEEEEECCCCEEEEEEEEECCCCCEEEEEEHHHH
SVV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]