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The map label for this gene is fixB [H]

Identifier: 182677484

GI number: 182677484

Start: 548256

End: 549368

Strand: Direct

Name: fixB [H]

Synonym: Bind_0488

Alternate gene names: 182677484

Gene position: 548256-549368 (Clockwise)

Preceding gene: 182677483

Following gene: 182677485

Centisome position: 13.15

GC content: 61.01

Gene sequence:

>1113_bases
ATGAATCAAAAAGCCCCAGCTAAAGCTCCGGCCGCCGCAGGAAACAGCCGCGCGAATACGAAGAAAGAGCTTCCGGAACA
TCTGAAGAGCTACAAGCACGTTTGGGTTCTTGTTGAACTCGAGCGTGGCGTCGTCCATCCCGTTTCCTGGGAATTGCTTG
GGGAAGGGCGCAAGCTCGCCGATAAGCTGAAGGTCGATCTGGCTGCTGTCGTCCTGGGCGCCCCCGGCGGCACGGCCGAC
GCGATCAAGGACAGCTTCGGTTACGGCGCCGACCTCTGCTATGTCGTCGAAAATCCGTCGCTCGTCGATTATCGCAACGA
GCCCTATACGACGGCCATGACCGAGCTGGTGAATAAGTACCAGCCGGAAATTCTGCTGATGGGCGCGACCAATCTCGGAC
GCGATCTGGCTGGTTCCGTGGCGACGACACTGCTGACCGGCCTGACGGCGGATTGCACGGAGCTCGACGTCGATAGCGAC
GGCTCGCTCGCCGCGACCCGTCCGACCTTCGGTGGTTCGCTGCTCTGCACGATCTATACGCTGAACTATCGCCCGCAAAT
GGCGACCATGCGCCCGCGCGTTGCGGCCATGCCGGTCTATCAGCCGGGTCGCACGGGCCGCGTCATCAATCATGAAGTGT
CGCTGCCGGAAGATTCGATCATTACCAAGGTCCTGTCCTTCCTGCCCGACAGCGCGACCGGCAAGGCCAATCTGCCTTAT
GCGGATGTCGTGGTGGCTGGTGGTCTCGGCATGCAAAATGCTGAAAATTATCAGTTGGTGCGCAATCTGGCCGAGGTCCT
GGGCGCTGAATGGGGTGGTTCGCGGCCCCTGGTGCAAAAAGGCTGGATTCCGTCCGACCGTCAGATCGGACAGACGGGTA
AGACCATCCGTCCGCGTCTCTATATTGCCGCGGGTATTTCCGGCGCCATTCAGCACCGCGTGGGCGTCGAGGGCGCGGAT
CTGATCGTCGCCATCAATACGGACCCGAATGCGCCGATCTTCGATTTCGCGCATGTCGGTATTGTCGCGGATGCAACGAA
AATATTGCCGGCGCTCACGGAAGCTTTCCGCAAGCGTCTCGCCCTGGCCGCCGCCCGGCCCGCGGCTGAGTAA

Upstream 100 bases:

>100_bases
GCGCTGATCGCGGAAATCAATCAGCGGCAGCCGGGTCTTGAACAAGACATTCAAAAATTGGCGGCGGGCTTCTAAGCCAT
TTTTCCTGAAGGATATAGCC

Downstream 100 bases:

>100_bases
AGGAGACGGACATGGCGGACGAAAAATTCGATGCGATCGTCGTAGGCGCCGGACCCTCCGGAAATGCTTGCGCTTATACT
CTGGCCACCCGTGGCCTCAA

Product: electron transfer flavoprotein alpha/beta-subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNQKAPAKAPAAAGNSRANTKKELPEHLKSYKHVWVLVELERGVVHPVSWELLGEGRKLADKLKVDLAAVVLGAPGGTAD
AIKDSFGYGADLCYVVENPSLVDYRNEPYTTAMTELVNKYQPEILLMGATNLGRDLAGSVATTLLTGLTADCTELDVDSD
GSLAATRPTFGGSLLCTIYTLNYRPQMATMRPRVAAMPVYQPGRTGRVINHEVSLPEDSIITKVLSFLPDSATGKANLPY
ADVVVAGGLGMQNAENYQLVRNLAEVLGAEWGGSRPLVQKGWIPSDRQIGQTGKTIRPRLYIAAGISGAIQHRVGVEGAD
LIVAINTDPNAPIFDFAHVGIVADATKILPALTEAFRKRLALAAARPAAE

Sequences:

>Translated_370_residues
MNQKAPAKAPAAAGNSRANTKKELPEHLKSYKHVWVLVELERGVVHPVSWELLGEGRKLADKLKVDLAAVVLGAPGGTAD
AIKDSFGYGADLCYVVENPSLVDYRNEPYTTAMTELVNKYQPEILLMGATNLGRDLAGSVATTLLTGLTADCTELDVDSD
GSLAATRPTFGGSLLCTIYTLNYRPQMATMRPRVAAMPVYQPGRTGRVINHEVSLPEDSIITKVLSFLPDSATGKANLPY
ADVVVAGGLGMQNAENYQLVRNLAEVLGAEWGGSRPLVQKGWIPSDRQIGQTGKTIRPRLYIAAGISGAIQHRVGVEGAD
LIVAINTDPNAPIFDFAHVGIVADATKILPALTEAFRKRLALAAARPAAE
>Mature_370_residues
MNQKAPAKAPAAAGNSRANTKKELPEHLKSYKHVWVLVELERGVVHPVSWELLGEGRKLADKLKVDLAAVVLGAPGGTAD
AIKDSFGYGADLCYVVENPSLVDYRNEPYTTAMTELVNKYQPEILLMGATNLGRDLAGSVATTLLTGLTADCTELDVDSD
GSLAATRPTFGGSLLCTIYTLNYRPQMATMRPRVAAMPVYQPGRTGRVINHEVSLPEDSIITKVLSFLPDSATGKANLPY
ADVVVAGGLGMQNAENYQLVRNLAEVLGAEWGGSRPLVQKGWIPSDRQIGQTGKTIRPRLYIAAGISGAIQHRVGVEGAD
LIVAINTDPNAPIFDFAHVGIVADATKILPALTEAFRKRLALAAARPAAE

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family [H]

Homologues:

Organism=Homo sapiens, GI189181759, Length=282, Percent_Identity=32.9787234042553, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4503607, Length=123, Percent_Identity=52.0325203252033, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787990, Length=273, Percent_Identity=32.967032967033, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786226, Length=256, Percent_Identity=32.421875, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87082157, Length=246, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506929, Length=231, Percent_Identity=38.0952380952381, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6325261, Length=302, Percent_Identity=31.7880794701987, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136898, Length=329, Percent_Identity=31.9148936170213, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24652801, Length=329, Percent_Identity=31.9148936170213, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha [H]

EC number: NA

Molecular weight: Translated: 39462; Mature: 39462

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKAPAKAPAAAGNSRANTKKELPEHLKSYKHVWVLVELERGVVHPVSWELLGEGRKLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHCCCHHHH
DKLKVDLAAVVLGAPGGTADAIKDSFGYGADLCYVVENPSLVDYRNEPYTTAMTELVNKY
HHHHHEEEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHC
QPEILLMGATNLGRDLAGSVATTLLTGLTADCTELDVDSDGSLAATRPTFGGSLLCTIYT
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEECCCCCCEEEEEEEE
LNYRPQMATMRPRVAAMPVYQPGRTGRVINHEVSLPEDSIITKVLSFLPDSATGKANLPY
ECCCCCHHHCCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCH
ADVVVAGGLGMQNAENYQLVRNLAEVLGAEWGGSRPLVQKGWIPSDRQIGQTGKTIRPRL
HHEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCEE
YIAAGISGAIQHRVGVEGADLIVAINTDPNAPIFDFAHVGIVADATKILPALTEAFRKRL
EEEECCCHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAARPAAE
HHHHCCCCCC
>Mature Secondary Structure
MNQKAPAKAPAAAGNSRANTKKELPEHLKSYKHVWVLVELERGVVHPVSWELLGEGRKLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHCCCHHHH
DKLKVDLAAVVLGAPGGTADAIKDSFGYGADLCYVVENPSLVDYRNEPYTTAMTELVNKY
HHHHHEEEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHC
QPEILLMGATNLGRDLAGSVATTLLTGLTADCTELDVDSDGSLAATRPTFGGSLLCTIYT
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEECCCCCCEEEEEEEE
LNYRPQMATMRPRVAAMPVYQPGRTGRVINHEVSLPEDSIITKVLSFLPDSATGKANLPY
ECCCCCHHHCCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCH
ADVVVAGGLGMQNAENYQLVRNLAEVLGAEWGGSRPLVQKGWIPSDRQIGQTGKTIRPRL
HHEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCEE
YIAAGISGAIQHRVGVEGADLIVAINTDPNAPIFDFAHVGIVADATKILPALTEAFRKRL
EEEECCCHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAARPAAE
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850088 [H]