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The map label for this gene is fixA [H]

Identifier: 182677483

GI number: 182677483

Start: 547376

End: 548230

Strand: Direct

Name: fixA [H]

Synonym: Bind_0487

Alternate gene names: 182677483

Gene position: 547376-548230 (Clockwise)

Preceding gene: 182677482

Following gene: 182677484

Centisome position: 13.13

GC content: 57.54

Gene sequence:

>855_bases
ATGCATATCGTCGTCTGCATTAAGCAGGTGCCGGACTCTGCGCAGATTCGTGTTCATCCTGTCACCAATACGATTATGCG
GCAGGGTGTGCCGACGATCATCAATCCTTACGATCTGTTCTCCCTTGAGGAGGCGTTGCGCTTGCGCGACAAGTTCGGTG
GCGAGGTGACTGTCGTGACCATGGGGCCGCCCATGGCCGATGCGTCCTTGCGTAAATGTCTGACTTTTGGAGCCGATCGC
GCCGTTCTGCTGACGGACCGCTTCTTCGCCGGTTCCGATACGCTCGCGACCTCCTATGCGCTTTCCTATTTGATCAAGAA
GGTCGGCGAGGAATGGGGTGCGCCCGATATTATCTTCTGCGGCAAGCAGACGATCGACGGGGATACTGCCCAGGTTGGTC
CTGGCATTGCCAAGCGCCTCAACCTCCTGCAGTTGACTTATATCTCCAAGATCGATGAATTCGATCTGGCCAAGCGTGAG
ATCACGCTGCAGCGTCGCGCCGAAGGCGGCATCCAGGTTCTCAAGACCAAATTGCCCTGCCTCGTGACCATGCTCGAGGG
TACGAATGACATGCGCCGTGGCTCCATCGAGGACGCGTTGCGCGGTGCTCGTTCGGAAATCGTCACCTGGAGCGCGGCAG
AGGCCGGTATTCCGGATCTCGCTCTGTGCGGCTTGCGTGGGTCTCCGACCATCGTGAAGAAAGTGTTCGCGCCGGCGCCG
CGTGATGAGAAGGCTTTCCAGATCGACGTGCAGGACAAATCCGCGAATGATATTTCCGAAGCGCTGATCGCGGAAATCAA
TCAGCGGCAGCCGGGTCTTGAACAAGACATTCAAAAATTGGCGGCGGGCTTCTAA

Upstream 100 bases:

>100_bases
ATACAATGTGAGGCTTTTGTTTCTACGTTGTTGTTCGGGGATGGTCCGAAATTTGCTGACTAAATCTATTCCACGTCATC
CATGATCGAGAGGTTTCATA

Downstream 100 bases:

>100_bases
GCCATTTTTCCTGAAGGATATAGCCATGAATCAAAAAGCCCCAGCTAAAGCTCCGGCCGCCGCAGGAAACAGCCGCGCGA
ATACGAAGAAAGAGCTTCCG

Product: electron transfer flavoprotein alpha/beta-subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFSLEEALRLRDKFGGEVTVVTMGPPMADASLRKCLTFGADR
AVLLTDRFFAGSDTLATSYALSYLIKKVGEEWGAPDIIFCGKQTIDGDTAQVGPGIAKRLNLLQLTYISKIDEFDLAKRE
ITLQRRAEGGIQVLKTKLPCLVTMLEGTNDMRRGSIEDALRGARSEIVTWSAAEAGIPDLALCGLRGSPTIVKKVFAPAP
RDEKAFQIDVQDKSANDISEALIAEINQRQPGLEQDIQKLAAGF

Sequences:

>Translated_284_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFSLEEALRLRDKFGGEVTVVTMGPPMADASLRKCLTFGADR
AVLLTDRFFAGSDTLATSYALSYLIKKVGEEWGAPDIIFCGKQTIDGDTAQVGPGIAKRLNLLQLTYISKIDEFDLAKRE
ITLQRRAEGGIQVLKTKLPCLVTMLEGTNDMRRGSIEDALRGARSEIVTWSAAEAGIPDLALCGLRGSPTIVKKVFAPAP
RDEKAFQIDVQDKSANDISEALIAEINQRQPGLEQDIQKLAAGF
>Mature_284_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFSLEEALRLRDKFGGEVTVVTMGPPMADASLRKCLTFGADR
AVLLTDRFFAGSDTLATSYALSYLIKKVGEEWGAPDIIFCGKQTIDGDTAQVGPGIAKRLNLLQLTYISKIDEFDLAKRE
ITLQRRAEGGIQVLKTKLPCLVTMLEGTNDMRRGSIEDALRGARSEIVTWSAAEAGIPDLALCGLRGSPTIVKKVFAPAP
RDEKAFQIDVQDKSANDISEALIAEINQRQPGLEQDIQKLAAGF

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family [H]

Homologues:

Organism=Homo sapiens, GI4503609, Length=219, Percent_Identity=28.310502283105, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI62420877, Length=206, Percent_Identity=28.1553398058252, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI87081682, Length=219, Percent_Identity=28.310502283105, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141345, Length=208, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321646, Length=187, Percent_Identity=29.9465240641711, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24651147, Length=183, Percent_Identity=32.2404371584699, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651145, Length=183, Percent_Identity=32.2404371584699, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF [H]

EC number: NA

Molecular weight: Translated: 31026; Mature: 31026

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFSLEEALRLRDKFGGEVTVVT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEE
MGPPMADASLRKCLTFGADRAVLLTDRFFAGSDTLATSYALSYLIKKVGEEWGAPDIIFC
CCCCCCHHHHHHHHHCCCCCEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
GKQTIDGDTAQVGPGIAKRLNLLQLTYISKIDEFDLAKREITLQRRAEGGIQVLKTKLPC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LVTMLEGTNDMRRGSIEDALRGARSEIVTWSAAEAGIPDLALCGLRGSPTIVKKVFAPAP
HHHHHCCCCCHHCCCHHHHHHHHHHHEEEEECHHCCCCCCEEECCCCCHHHHHHHHCCCC
RDEKAFQIDVQDKSANDISEALIAEINQRQPGLEQDIQKLAAGF
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFSLEEALRLRDKFGGEVTVVT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEE
MGPPMADASLRKCLTFGADRAVLLTDRFFAGSDTLATSYALSYLIKKVGEEWGAPDIIFC
CCCCCCHHHHHHHHHCCCCCEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
GKQTIDGDTAQVGPGIAKRLNLLQLTYISKIDEFDLAKREITLQRRAEGGIQVLKTKLPC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LVTMLEGTNDMRRGSIEDALRGARSEIVTWSAAEAGIPDLALCGLRGSPTIVKKVFAPAP
HHHHHCCCCCHHCCCHHHHHHHHHHHEEEEECHHCCCCCCEEECCCCCHHHHHHHHCCCC
RDEKAFQIDVQDKSANDISEALIAEINQRQPGLEQDIQKLAAGF
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]