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The map label for this gene is glpK

Identifier: 182677376

GI number: 182677376

Start: 438366

End: 439889

Strand: Direct

Name: glpK

Synonym: Bind_0380

Alternate gene names: 182677376

Gene position: 438366-439889 (Clockwise)

Preceding gene: 182677375

Following gene: 182677377

Centisome position: 10.51

GC content: 57.09

Gene sequence:

>1524_bases
GTGACGAAATATGTTGCCGTGATCGATCAGGGCACAACCAGCACACGCTGCATGGTTTTCAACAAACAGGGTGAAATTGT
CGCCCAGCATCAATTGGAACATGAACAGATCTGTCCGCAAGCCGGCTGGGTCGAACATGATCCGCTGGAAATCTGGGAAC
GGACCAAGGATGTCATCCATGGATCTGTCGCCAAGGCCGGGCTGGTTGCCGCCGATATAGCGGCCATCGGCATTACCAAC
CAGCGTGAAACGACGATGATCTGGAATCGCAAGACCGGCCAGCCCTATGGCAATGCCATTGTCTGGCAGGATACGCGCAC
GGATATCGTGTGCAACCAGATGTCTGCCGAGGGCGGCCAGAACCGTTTCCAGGCCAAGACGGGCCTGCCGCTCGCCACTT
ATTTTTCAGGGCCGAAGATCCGCTGGATGCTCGATCATTATCCGGGCCTGCGCCAGGATGCGGAAAAGGGCGAGGCTCTA
TTCGGCAATATGGATTCCTGGCTGATCTGGAAATTGACCGGAGGGCCTGGGCCTGCGGCCGTGCATGTGACGGATGTGAC
CAATGCTTCCCGGACCATGCTGATGAATCTCAAAACGCTCGATTGGGATGAGGAATTGCTGTCGGCCTTCGCTATTCCGC
GCGCCATGCTGCCGAGCATACGGCCGTCTTCAGATCCTGAATTCTATGGCTTCACCCGCCAGGATGGCCCTTTTGGCGGT
GAAATCCCGATCTGCGGCGATCTTGGCGATCAGCAGGCGGCGACGGTCGGACAAGTCTGTTTCGATGCGGGAGAAGCCAA
AAATACCTATGGCACCGGCTGTTTCATGCTCATGAATACGGGCACGGAAATGGTCCATTCGAACCATGGGCTCTTGACGA
CGGTCTGTTATCAATTCGGCCATGAGAAGCCGCATTATGCACTGGAAGGCTCGGTGGCGATTGCCGGCGCCCTGGTCCAA
TGGCTGCGCGATCGTATGCGCGTTATCAATACGGCGGCCGATATCGAAAACCTGGCCAAACTCGTTCCCGATAATGGCGG
CATGTATTTCGTGCCGGCCTTTTCCGGGCTTTTCGCTCCCTATTGGCGCAGCGAGGCGCGCGGCCTCATCATCGGCATGA
CCCGTTTCATCAATCGCGGCCATTTTGCCCGCGCGAGCCTGGAAGCCGCTGCCTACCAGACCCGCGAAGTGGTGGATGCC
ATGCAGGCTGACTCCAATGTCTCGCTGAAAGTCTTGAAAGTCGATGGCGGGATGACCGCCAATGATCTTTTGATGCAGAT
CCAGGCTGATGTTCTGGGTGTGCCAGTGGTGCGGCCGAAAGTGGCGGAAAGCACGGCTCTTGGGGCCGCCTATGCGGCTG
GCCTTGCCATTGGCTATTGGAAGAATAGTGACGACCTGTGCCAGAATTGGGGTGTCGACAAGAGTTGGAAGCCCTCGGGT
GATGATACTTTGCGCACTAAAAATTACGCCATGTGGAAAAAGGCCGTGACCCGCACCTTCGACTGGCTCGATGAGAGCGC
GTGA

Upstream 100 bases:

>100_bases
TCGAGCAGGGTGAAGATGAGAGCATTTTTCATTTCACCCGCTCCTTGTGATTGCCATCGATCACATCCATGGCACGATAA
AGATTAAGGGAGGAATGATC

Downstream 100 bases:

>100_bases
TCATAGGCCTCGCCGGAAAACAGGGAGGCCGCACCGCGAATCCTTGGGGAACGATGATCCTCAAGGAGCATTGACTCCCA
GGGTGGAGGCGGCTCACCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTKYVAVIDQGTTSTRCMVFNKQGEIVAQHQLEHEQICPQAGWVEHDPLEIWERTKDVIHGSVAKAGLVAADIAAIGITN
QRETTMIWNRKTGQPYGNAIVWQDTRTDIVCNQMSAEGGQNRFQAKTGLPLATYFSGPKIRWMLDHYPGLRQDAEKGEAL
FGNMDSWLIWKLTGGPGPAAVHVTDVTNASRTMLMNLKTLDWDEELLSAFAIPRAMLPSIRPSSDPEFYGFTRQDGPFGG
EIPICGDLGDQQAATVGQVCFDAGEAKNTYGTGCFMLMNTGTEMVHSNHGLLTTVCYQFGHEKPHYALEGSVAIAGALVQ
WLRDRMRVINTAADIENLAKLVPDNGGMYFVPAFSGLFAPYWRSEARGLIIGMTRFINRGHFARASLEAAAYQTREVVDA
MQADSNVSLKVLKVDGGMTANDLLMQIQADVLGVPVVRPKVAESTALGAAYAAGLAIGYWKNSDDLCQNWGVDKSWKPSG
DDTLRTKNYAMWKKAVTRTFDWLDESA

Sequences:

>Translated_507_residues
MTKYVAVIDQGTTSTRCMVFNKQGEIVAQHQLEHEQICPQAGWVEHDPLEIWERTKDVIHGSVAKAGLVAADIAAIGITN
QRETTMIWNRKTGQPYGNAIVWQDTRTDIVCNQMSAEGGQNRFQAKTGLPLATYFSGPKIRWMLDHYPGLRQDAEKGEAL
FGNMDSWLIWKLTGGPGPAAVHVTDVTNASRTMLMNLKTLDWDEELLSAFAIPRAMLPSIRPSSDPEFYGFTRQDGPFGG
EIPICGDLGDQQAATVGQVCFDAGEAKNTYGTGCFMLMNTGTEMVHSNHGLLTTVCYQFGHEKPHYALEGSVAIAGALVQ
WLRDRMRVINTAADIENLAKLVPDNGGMYFVPAFSGLFAPYWRSEARGLIIGMTRFINRGHFARASLEAAAYQTREVVDA
MQADSNVSLKVLKVDGGMTANDLLMQIQADVLGVPVVRPKVAESTALGAAYAAGLAIGYWKNSDDLCQNWGVDKSWKPSG
DDTLRTKNYAMWKKAVTRTFDWLDESA
>Mature_506_residues
TKYVAVIDQGTTSTRCMVFNKQGEIVAQHQLEHEQICPQAGWVEHDPLEIWERTKDVIHGSVAKAGLVAADIAAIGITNQ
RETTMIWNRKTGQPYGNAIVWQDTRTDIVCNQMSAEGGQNRFQAKTGLPLATYFSGPKIRWMLDHYPGLRQDAEKGEALF
GNMDSWLIWKLTGGPGPAAVHVTDVTNASRTMLMNLKTLDWDEELLSAFAIPRAMLPSIRPSSDPEFYGFTRQDGPFGGE
IPICGDLGDQQAATVGQVCFDAGEAKNTYGTGCFMLMNTGTEMVHSNHGLLTTVCYQFGHEKPHYALEGSVAIAGALVQW
LRDRMRVINTAADIENLAKLVPDNGGMYFVPAFSGLFAPYWRSEARGLIIGMTRFINRGHFARASLEAAAYQTREVVDAM
QADSNVSLKVLKVDGGMTANDLLMQIQADVLGVPVVRPKVAESTALGAAYAAGLAIGYWKNSDDLCQNWGVDKSWKPSGD
DTLRTKNYAMWKKAVTRTFDWLDESA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=508, Percent_Identity=49.2125984251969, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI189409120, Length=508, Percent_Identity=49.2125984251969, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=48.9278752436647, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI41393575, Length=508, Percent_Identity=49.0157480314961, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI88196792, Length=520, Percent_Identity=29.6153846153846, Blast_Score=264, Evalue=2e-70,
Organism=Escherichia coli, GI1790361, Length=501, Percent_Identity=53.0938123752495, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1790005, Length=501, Percent_Identity=23.1536926147705, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789987, Length=507, Percent_Identity=24.4575936883629, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1787789, Length=483, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789168, Length=480, Percent_Identity=22.9166666666667, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=508, Percent_Identity=43.8976377952756, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=23.2245681381958, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=27.2388059701493, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=39.8916967509025, Blast_Score=355, Evalue=9e-99,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=47.5442043222004, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24655555, Length=517, Percent_Identity=42.3597678916828, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655560, Length=517, Percent_Identity=42.3597678916828, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655570, Length=517, Percent_Identity=42.3597678916828, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655574, Length=517, Percent_Identity=42.3597678916828, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655565, Length=517, Percent_Identity=42.3597678916828, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24654573, Length=463, Percent_Identity=47.5161987041037, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI19922058, Length=537, Percent_Identity=34.6368715083799, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24652871, Length=496, Percent_Identity=34.4758064516129, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=29.4339622641509, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24656367, Length=519, Percent_Identity=29.8651252408478, Blast_Score=225, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24656372, Length=398, Percent_Identity=29.8994974874372, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45552909, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_BEII9 (B2IE09)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831522.1
- ProteinModelPortal:   B2IE09
- GeneID:   6199560
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0380
- HOGENOM:   HBG511469
- OMA:   KILPNVR
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55759; Mature: 55628

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 250-250 BINDING 272-272 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVAVIDQGTTSTRCMVFNKQGEIVAQHQLEHEQICPQAGWVEHDPLEIWERTKDVIH
CCCEEEEEECCCCCCEEEEECCCCCEEEHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHH
GSVAKAGLVAADIAAIGITNQRETTMIWNRKTGQPYGNAIVWQDTRTDIVCNQMSAEGGQ
HHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCCCC
NRFQAKTGLPLATYFSGPKIRWMLDHYPGLRQDAEKGEALFGNMDSWLIWKLTGGPGPAA
CCEEHHCCCEEEEECCCCEEEEEECCCCCCCCHHHCCCCEECCCCCEEEEEEECCCCCEE
VHVTDVTNASRTMLMNLKTLDWDEELLSAFAIPRAMLPSIRPSSDPEFYGFTRQDGPFGG
EEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEEECCCCCCCC
EIPICGDLGDQQAATVGQVCFDAGEAKNTYGTGCFMLMNTGTEMVHSNHGLLTTVCYQFG
CCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHCCCCHHHHHHHHHC
HEKPHYALEGSVAIAGALVQWLRDRMRVINTAADIENLAKLVPDNGGMYFVPAFSGLFAP
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHCH
YWRSEARGLIIGMTRFINRGHFARASLEAAAYQTREVVDAMQADSNVSLKVLKVDGGMTA
HHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
NDLLMQIQADVLGVPVVRPKVAESTALGAAYAAGLAIGYWKNSDDLCQNWGVDKSWKPSG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCCCCC
DDTLRTKNYAMWKKAVTRTFDWLDESA
CCCEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKYVAVIDQGTTSTRCMVFNKQGEIVAQHQLEHEQICPQAGWVEHDPLEIWERTKDVIH
CCEEEEEECCCCCCEEEEECCCCCEEEHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHH
GSVAKAGLVAADIAAIGITNQRETTMIWNRKTGQPYGNAIVWQDTRTDIVCNQMSAEGGQ
HHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCCCC
NRFQAKTGLPLATYFSGPKIRWMLDHYPGLRQDAEKGEALFGNMDSWLIWKLTGGPGPAA
CCEEHHCCCEEEEECCCCEEEEEECCCCCCCCHHHCCCCEECCCCCEEEEEEECCCCCEE
VHVTDVTNASRTMLMNLKTLDWDEELLSAFAIPRAMLPSIRPSSDPEFYGFTRQDGPFGG
EEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEEECCCCCCCC
EIPICGDLGDQQAATVGQVCFDAGEAKNTYGTGCFMLMNTGTEMVHSNHGLLTTVCYQFG
CCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHCCCCHHHHHHHHHC
HEKPHYALEGSVAIAGALVQWLRDRMRVINTAADIENLAKLVPDNGGMYFVPAFSGLFAP
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHCH
YWRSEARGLIIGMTRFINRGHFARASLEAAAYQTREVVDAMQADSNVSLKVLKVDGGMTA
HHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
NDLLMQIQADVLGVPVVRPKVAESTALGAAYAAGLAIGYWKNSDDLCQNWGVDKSWKPSG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCCCCC
DDTLRTKNYAMWKKAVTRTFDWLDESA
CCCEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA