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The map label for this gene is glpF [H]

Identifier: 182677375

GI number: 182677375

Start: 437444

End: 438172

Strand: Direct

Name: glpF [H]

Synonym: Bind_0379

Alternate gene names: 182677375

Gene position: 437444-438172 (Clockwise)

Preceding gene: 182677374

Following gene: 182677376

Centisome position: 10.49

GC content: 55.01

Gene sequence:

>729_bases
ATGCACGCTACATCTATATTTCTGGGTGAGTTCCTGGGCACTATGATCCTTATCCTGTTGGGTGATGGGGTCGTGGCCAA
TGTCCTGCTCGAAAAATCGAAAGGGCAGAATTCCGGCTGGATGGTCATTGCCGCCGGCTGGGGTTTTGGTGTCGCCATTG
CCGTTTATATCTCCGGCTGGATCAGCGGCGCCCATCTCAACCCCGCCATTACAATCGGCTTCCTGCTGGTCGGCAAGGTG
ACACCCGCCTTGGTTCCTGCCTATTTCGCTGGCCAGTTCGCCGGCGCCATAGCAGGCGCTGTTCTGGTCTATTTGGCCTA
TCTGCCGCATTGGGAAAAAACGAAAGATGGGGATCTCAAGCTTGCTGTCTTCTGCACGGCTCCGGCGATCCGCCATTCCG
CTTCAAATCTGCTCTGTGAATTGATCGGCACGGCCATTCTCGTCGTCGGTGTTTTGGGTATTTTCAATACCAATAATGGG
ATCGGCAGCGGTTTTGGCCCCTATGCCGTCGGTATTCTCGTCTTCAGCATTGGTCTCTCGCTTGGCGGTCCCACGGGTTA
TGCCATTAATCCCGCGCGCGACCTTGGGCCGCGCATTGCCCATGCCATTCTGCCGATTACAGGCAAGCGTGATTCAGATT
GGAATTATGCCTGGATACCGGTTATCGGCCCTTTGATTGGCGGCGGTCTGGGGGCGCTGCTCTATTACGGTTTTGTTGGT
CTTCTTTAA

Upstream 100 bases:

>100_bases
AGGCTTACAAAGGGGCGATGCAGGCGGCAATCATGAATGAAAGAGCTGGGACAATAACAACCCGGCCTTCGGAGAAAATC
AAATGAGGGAGGAAAGATGA

Downstream 100 bases:

>100_bases
ATTTCTCACCCAAACCGTGATTGTGATCTGGAGCCTTGATCGCGCATGGGCCGGCTCTGTCCTATCCATTGAGATATTTG
AAGCATGGATTTGTCGAGCA

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MHATSIFLGEFLGTMILILLGDGVVANVLLEKSKGQNSGWMVIAAGWGFGVAIAVYISGWISGAHLNPAITIGFLLVGKV
TPALVPAYFAGQFAGAIAGAVLVYLAYLPHWEKTKDGDLKLAVFCTAPAIRHSASNLLCELIGTAILVVGVLGIFNTNNG
IGSGFGPYAVGILVFSIGLSLGGPTGYAINPARDLGPRIAHAILPITGKRDSDWNYAWIPVIGPLIGGGLGALLYYGFVG
LL

Sequences:

>Translated_242_residues
MHATSIFLGEFLGTMILILLGDGVVANVLLEKSKGQNSGWMVIAAGWGFGVAIAVYISGWISGAHLNPAITIGFLLVGKV
TPALVPAYFAGQFAGAIAGAVLVYLAYLPHWEKTKDGDLKLAVFCTAPAIRHSASNLLCELIGTAILVVGVLGIFNTNNG
IGSGFGPYAVGILVFSIGLSLGGPTGYAINPARDLGPRIAHAILPITGKRDSDWNYAWIPVIGPLIGGGLGALLYYGFVG
LL
>Mature_242_residues
MHATSIFLGEFLGTMILILLGDGVVANVLLEKSKGQNSGWMVIAAGWGFGVAIAVYISGWISGAHLNPAITIGFLLVGKV
TPALVPAYFAGQFAGAIAGAVLVYLAYLPHWEKTKDGDLKLAVFCTAPAIRHSASNLLCELIGTAILVVGVLGIFNTNNG
IGSGFGPYAVGILVFSIGLSLGGPTGYAINPARDLGPRIAHAILPITGKRDSDWNYAWIPVIGPLIGGGLGALLYYGFVG
LL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=241, Percent_Identity=37.7593360995851, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI4826645, Length=248, Percent_Identity=35.0806451612903, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=34.1463414634146, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=35.5102040816327, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI4502179, Length=232, Percent_Identity=31.8965517241379, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI6912506, Length=199, Percent_Identity=33.6683417085427, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI37694062, Length=232, Percent_Identity=30.6034482758621, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310133356, Length=219, Percent_Identity=31.5068493150685, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI310114181, Length=219, Percent_Identity=31.5068493150685, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI4755125, Length=222, Percent_Identity=31.5315315315315, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4502181, Length=222, Percent_Identity=31.5315315315315, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1790362, Length=261, Percent_Identity=35.632183908046, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994009, Length=241, Percent_Identity=35.2697095435685, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=247, Percent_Identity=35.6275303643725, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531431, Length=242, Percent_Identity=32.6446280991736, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=242, Percent_Identity=32.6446280991736, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=34.2741935483871, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17533613, Length=243, Percent_Identity=29.6296296296296, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=243, Percent_Identity=29.6296296296296, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=31.7796610169492, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=243, Percent_Identity=32.0987654320988, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI212646268, Length=261, Percent_Identity=26.8199233716475, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71993722, Length=237, Percent_Identity=30.379746835443, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6321054, Length=204, Percent_Identity=35.7843137254902, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322985, Length=277, Percent_Identity=28.5198555956679, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652747, Length=231, Percent_Identity=30.7359307359307, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45551084, Length=231, Percent_Identity=30.7359307359307, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24762342, Length=241, Percent_Identity=30.2904564315353, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550503, Length=236, Percent_Identity=25, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922828, Length=233, Percent_Identity=28.3261802575107, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24762344, Length=233, Percent_Identity=31.3304721030043, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762346, Length=233, Percent_Identity=31.3304721030043, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762348, Length=233, Percent_Identity=31.3304721030043, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130305, Length=233, Percent_Identity=31.3304721030043, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25034; Mature: 25034

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHATSIFLGEFLGTMILILLGDGVVANVLLEKSKGQNSGWMVIAAGWGFGVAIAVYISGW
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHH
ISGAHLNPAITIGFLLVGKVTPALVPAYFAGQFAGAIAGAVLVYLAYLPHWEKTKDGDLK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LAVFCTAPAIRHSASNLLCELIGTAILVVGVLGIFNTNNGIGSGFGPYAVGILVFSIGLS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LGGPTGYAINPARDLGPRIAHAILPITGKRDSDWNYAWIPVIGPLIGGGLGALLYYGFVG
CCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHH
LL
CC
>Mature Secondary Structure
MHATSIFLGEFLGTMILILLGDGVVANVLLEKSKGQNSGWMVIAAGWGFGVAIAVYISGW
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHH
ISGAHLNPAITIGFLLVGKVTPALVPAYFAGQFAGAIAGAVLVYLAYLPHWEKTKDGDLK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LAVFCTAPAIRHSASNLLCELIGTAILVVGVLGIFNTNNGIGSGFGPYAVGILVFSIGLS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LGGPTGYAINPARDLGPRIAHAILPITGKRDSDWNYAWIPVIGPLIGGGLGALLYYGFVG
CCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHH
LL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]