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The map label for this gene is dctD [H]

Identifier: 182677184

GI number: 182677184

Start: 220094

End: 221434

Strand: Direct

Name: dctD [H]

Synonym: Bind_0185

Alternate gene names: 182677184

Gene position: 220094-221434 (Clockwise)

Preceding gene: 182677183

Following gene: 182677185

Centisome position: 5.28

GC content: 50.04

Gene sequence:

>1341_bases
ATGGATGTAGCCTTTATTGATGATGATGAGATACTGTGCGCGGCCAATGTTCAAGCCCTTGTTCTGGCAGGGCTTGATGT
CGTGACATATCATTCTGCCATGGCCGCGCTTGACGAACTTAACTCTGAGTTCACAGGCGTAGTGGTTAGCGATATCCGTA
TGCCAGGCATTGATGGCCTGCAATTTTTCAAATATCTTAAGAAAATCGACCCGGATCTTCCCGTCATCCTCATCACGGGT
CATGGTGATATCTCTATGGCCGTCGACGCTCTGCGCGATGGAGTTTATGACTTTATCTCCAAGCCTTATACATTCGACCG
TCTCCTCGGAAGTATTCGGCATGCACTGGAAAGCCGTCGACTTGTGCTAGAGAACCGGAGACTCAAACGACAAATAGAAG
CCTCTGCGATCAATCTTCCATTGCTTGGAAGCTCGCCTGCGATGGAACGACTCAGACAAACGTTGCATCAGCTTGCGGAT
GCTGATGTTGACGTTCTCATCGAAGGAGAAACCGGCACTGGAAAAGAAGTCGTCGCGAATACTTTGCATCAATGGAGTCA
CCGCTCCAATCGGCCCTTTGTCGCGTTGAACTGTGGTGCGTTGCCAGAAAGTGTGATCGAGAGCGAGCTATTCGGACACG
AAGCAGGTGCCTTTACCGGTGCCGTGAAAAAGCGGGTGGGACGTATTGAGCATGCCCATGGGGGTACACTTTTCCTTGAT
GAAATTGAGTCAATGCCACCTGTCCTGCAAGTCAAGTTATTGCGGGTGCTTGAAACGCGTGAAATTGCTCCTCTTGGAAC
GAACAATATCCGTCACGTCGATATACGTGTAGTAGCAGCATCTAAGACCGACCTACGAACGCTCGTCGAACGCAATTCCT
TTCGTGAAGATCTCTATTATCGTCTTCATGTTGCTTACATACACATTCCACCACTCAGAGAGCGTAAGGCCGATGTGTCA
CTTCTATTCGGTTATTTCCTGGCAAATGCGGCCAAGCGTTTCCGGCGCTCGCCTGTCGCGATCACCGAAATGGTACGGCA
TCATCTGAATTCCTATGATTGGCCGGGCAATGTTCGCGAACTCGTTCACTATGCTGAACGCGTAGCTCTTGGTCTCGTGG
AAGAGCAAAAGCAGGTCTCAGCAGAACCTGAGGCGACGCTACGGGAACGAATGGATGCCTTTGAAGCAGATCAAATCCGC
GCGGCCTTGGCTGCAAACAGCGGAGATATACAGGAAACAGTCAAGTCTCTCGGTATCGCCCGTAAGACCCTCTACGACAA
ATTGCACCGATATAGCATCGACCAGACTATTTTCAGAGGTACTCATACCAAGTCTCTTTGA

Upstream 100 bases:

>100_bases
TCCTGATTTCCCACGACATCCTTAATGAGTTCAACGGTACGCTCGCTGTGGAAAATTGTGACGGCGCCACGTTGACCATT
ACCCTACCGAGAGTCAATTG

Downstream 100 bases:

>100_bases
TTATAATGCATGTGAGGCGGTAGCTGCCTGTTTTTTGCGTTAAACAATCAAAACATCCCAGGTTAATGGCGGCGTCATAG
TTAACGCATCAGTCAAATTG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDVAFIDDDEILCAANVQALVLAGLDVVTYHSAMAALDELNSEFTGVVVSDIRMPGIDGLQFFKYLKKIDPDLPVILITG
HGDISMAVDALRDGVYDFISKPYTFDRLLGSIRHALESRRLVLENRRLKRQIEASAINLPLLGSSPAMERLRQTLHQLAD
ADVDVLIEGETGTGKEVVANTLHQWSHRSNRPFVALNCGALPESVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFLD
EIESMPPVLQVKLLRVLETREIAPLGTNNIRHVDIRVVAASKTDLRTLVERNSFREDLYYRLHVAYIHIPPLRERKADVS
LLFGYFLANAAKRFRRSPVAITEMVRHHLNSYDWPGNVRELVHYAERVALGLVEEQKQVSAEPEATLRERMDAFEADQIR
AALAANSGDIQETVKSLGIARKTLYDKLHRYSIDQTIFRGTHTKSL

Sequences:

>Translated_446_residues
MDVAFIDDDEILCAANVQALVLAGLDVVTYHSAMAALDELNSEFTGVVVSDIRMPGIDGLQFFKYLKKIDPDLPVILITG
HGDISMAVDALRDGVYDFISKPYTFDRLLGSIRHALESRRLVLENRRLKRQIEASAINLPLLGSSPAMERLRQTLHQLAD
ADVDVLIEGETGTGKEVVANTLHQWSHRSNRPFVALNCGALPESVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFLD
EIESMPPVLQVKLLRVLETREIAPLGTNNIRHVDIRVVAASKTDLRTLVERNSFREDLYYRLHVAYIHIPPLRERKADVS
LLFGYFLANAAKRFRRSPVAITEMVRHHLNSYDWPGNVRELVHYAERVALGLVEEQKQVSAEPEATLRERMDAFEADQIR
AALAANSGDIQETVKSLGIARKTLYDKLHRYSIDQTIFRGTHTKSL
>Mature_446_residues
MDVAFIDDDEILCAANVQALVLAGLDVVTYHSAMAALDELNSEFTGVVVSDIRMPGIDGLQFFKYLKKIDPDLPVILITG
HGDISMAVDALRDGVYDFISKPYTFDRLLGSIRHALESRRLVLENRRLKRQIEASAINLPLLGSSPAMERLRQTLHQLAD
ADVDVLIEGETGTGKEVVANTLHQWSHRSNRPFVALNCGALPESVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFLD
EIESMPPVLQVKLLRVLETREIAPLGTNNIRHVDIRVVAASKTDLRTLVERNSFREDLYYRLHVAYIHIPPLRERKADVS
LLFGYFLANAAKRFRRSPVAITEMVRHHLNSYDWPGNVRELVHYAERVALGLVEEQKQVSAEPEATLRERMDAFEADQIR
AALAANSGDIQETVKSLGIARKTLYDKLHRYSIDQTIFRGTHTKSL

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=40.0900900900901, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1790437, Length=443, Percent_Identity=39.9548532731377, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1788550, Length=451, Percent_Identity=37.4722838137472, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=35.6837606837607, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1789087, Length=331, Percent_Identity=40.785498489426, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87082117, Length=247, Percent_Identity=48.582995951417, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI87082152, Length=289, Percent_Identity=45.6747404844291, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=39.7515527950311, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=312, Percent_Identity=41.9871794871795, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=45.4148471615721, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=35.1612903225806, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=33.6569579288026, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789828, Length=234, Percent_Identity=34.1880341880342, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49968; Mature: 49968

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAFIDDDEILCAANVQALVLAGLDVVTYHSAMAALDELNSEFTGVVVSDIRMPGIDGL
CCCEEECCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHCCHHCEEEEECCCCCCCCHH
QFFKYLKKIDPDLPVILITGHGDISMAVDALRDGVYDFISKPYTFDRLLGSIRHALESRR
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LVLENRRLKRQIEASAINLPLLGSSPAMERLRQTLHQLADADVDVLIEGETGTGKEVVAN
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
TLHQWSHRSNRPFVALNCGALPESVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFLD
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEHH
EIESMPPVLQVKLLRVLETREIAPLGTNNIRHVDIRVVAASKTDLRTLVERNSFREDLYY
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHEE
RLHVAYIHIPPLRERKADVSLLFGYFLANAAKRFRRSPVAITEMVRHHLNSYDWPGNVRE
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LVHYAERVALGLVEEQKQVSAEPEATLRERMDAFEADQIRAALAANSGDIQETVKSLGIA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RKTLYDKLHRYSIDQTIFRGTHTKSL
HHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDVAFIDDDEILCAANVQALVLAGLDVVTYHSAMAALDELNSEFTGVVVSDIRMPGIDGL
CCCEEECCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHCCHHCEEEEECCCCCCCCHH
QFFKYLKKIDPDLPVILITGHGDISMAVDALRDGVYDFISKPYTFDRLLGSIRHALESRR
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LVLENRRLKRQIEASAINLPLLGSSPAMERLRQTLHQLADADVDVLIEGETGTGKEVVAN
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
TLHQWSHRSNRPFVALNCGALPESVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFLD
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEHH
EIESMPPVLQVKLLRVLETREIAPLGTNNIRHVDIRVVAASKTDLRTLVERNSFREDLYY
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHEE
RLHVAYIHIPPLRERKADVSLLFGYFLANAAKRFRRSPVAITEMVRHHLNSYDWPGNVRE
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LVHYAERVALGLVEEQKQVSAEPEATLRERMDAFEADQIRAALAANSGDIQETVKSLGIA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RKTLYDKLHRYSIDQTIFRGTHTKSL
HHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]