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The map label for this gene is dctB [H]

Identifier: 182677183

GI number: 182677183

Start: 218280

End: 220094

Strand: Direct

Name: dctB [H]

Synonym: Bind_0184

Alternate gene names: 182677183

Gene position: 218280-220094 (Clockwise)

Preceding gene: 182677180

Following gene: 182677184

Centisome position: 5.23

GC content: 54.6

Gene sequence:

>1815_bases
ATGGGCATTGCGCGATGGCACTCGATGCCTTGCCGCTTGCTGCTGTTCGCGCTAGCCGCCATTTTGATCCTCGGATTTGA
CGGCGCCGCCCGCTATGTGGCTCAGCGATCCGTGCTCTCGGAACTCGGCGCAGCGGCGAAAATCGCGGCGAGCCTCCGAG
TCGCTATGTTGCGCAATGAAATTGAAAAGCAGCGCACACTTCCCATCGTGCTGGCACAAGATCCGGATGTTCGGGCAACG
CTCGAAAGAGGCGGATCTGTCCGCCTTGCTGCCCTAAACGCCAAGCTTGAGGCATTGGCTGCCGCCACTCGTGCTGGCGC
AATTTACCTCCTGGATGCTAACGGCATCGCGATCGCCGCGAGCAATTATCGTATGCCCACCAGTTTCGTCGGGACCAATT
ATGCGTTCCGCCCTTATTTCAAAAGGACCCTGGAGGAAGGAAGTGCCGAGCATTTTGCCTTTGGTACCGTTAGTCATAAA
CCAGGGCTCTACCTGACACAACGGTTGGACGGTCCAACCGGGCCGCTTGGTATGCTGATTGTGAAAGCGGAATTCGACGC
GGTCGAGGCTGAATGGCGGCACGGCACGGCGCCTACTTTCGTCAGCGACGAGCGTGGCATCATTCTGGTGACAAGTGAAC
CGTCTTGGCGTTTTCGCACAAAGACGCCGATCCCGGTCGAGCGTCGCGCCGAAATCCTGGCCAATCTGCAATTTGGTGAT
GCTAAACTCGACCTTCTCCCACTACACACCAACACTGGAAGTATAGGTCTCGTGGAAGCGACATTGCCTGGTGAATACAG
GCCTCGCTACTTCGTTGAAGGGGGCAGCAAGGTTTCTTCGATTGACTGGACGCTCCACGTTTTGACGCCGACAGAGACCA
CGATGAAACTAACGGTCACGGCTTGGCGCTCCCTAACCTTGCTTTTAGTCACCGTAGCGCTTGGGGTGGTGGGATTATGG
TTGTCTCGACAGCATCGCTTGGTCGAGGAAAGGAAACAGCACGCGACGGCGCGCCGGGAGCTCGAGAATATGGTTGAACA
CCGTACCAAGGAGCTTCGCCACATCAATGAACGTTTGACTGCTGAAGTCGAGAAACGCGAACATGCGCAAATAGCTGTAC
ATAATCTGCAGGACGAATTGGTTCAGGCAAGTAAGCTTGCCGTTCTCGGTCAGATCGCAGCAAGCGTAGCTCATGAAGTC
AATCAACCAGTCGCCGCGATTCGGACCTTCGCCGAGAATTCACAAGTTCTTCTCATTCGAGGTGATACAGGCACTGCAAG
AATCAATCTCTCGACGATTGCAGGTCTGACAGAACGCATCGGCACTATCATGGATGAACTTCGTGCTTTCTCACGCAAAA
CAACAAGTGAGAAGGAACCCATCTCCCTGCGGGAGACCATCGACGGAGCGCTTCTTCTGGTCGGACATCGTCTCAAGCAG
CAGACAATTAATTTATTCGTCGATCTGGCAGATGCGGATATTGTGGTGGCGGCAGAGCGTATCCGACTCGAACAAGTTTT
TGTAAACTTATTGCAGAACGCCATCGAGGCTGTGTCCAATCAGCCCGATGGATCCATCCAGATCTTGGCCCGACCAACGG
CGGAAAACGTTATCGTGACGATCGCCGACAATGGGCCAGGCTTTCCCGATGCCGTCATGACTGCTCTTTTCATGCCCTTC
ACGACCACTAAGCCAGATGGAGTTGGGCTCGGACTCCTGATTTCCCACGACATCCTTAATGAGTTCAACGGTACGCTCGC
TGTGGAAAATTGTGACGGCGCCACGTTGACCATTACCCTACCGAGAGTCAATTGA

Upstream 100 bases:

>100_bases
AAATCGTGCGGAATTACGCACAAATAGAAAAAGACGATGCGGAGAATTACACAGATAATCGGGATCTAAAATTTTGGGGC
TAGCTTGGATTAGGCAGAGC

Downstream 100 bases:

>100_bases
TGGATGTAGCCTTTATTGATGATGATGAGATACTGTGCGCGGCCAATGTTCAAGCCCTTGTTCTGGCAGGGCTTGATGTC
GTGACATATCATTCTGCCAT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MGIARWHSMPCRLLLFALAAILILGFDGAARYVAQRSVLSELGAAAKIAASLRVAMLRNEIEKQRTLPIVLAQDPDVRAT
LERGGSVRLAALNAKLEALAAATRAGAIYLLDANGIAIAASNYRMPTSFVGTNYAFRPYFKRTLEEGSAEHFAFGTVSHK
PGLYLTQRLDGPTGPLGMLIVKAEFDAVEAEWRHGTAPTFVSDERGIILVTSEPSWRFRTKTPIPVERRAEILANLQFGD
AKLDLLPLHTNTGSIGLVEATLPGEYRPRYFVEGGSKVSSIDWTLHVLTPTETTMKLTVTAWRSLTLLLVTVALGVVGLW
LSRQHRLVEERKQHATARRELENMVEHRTKELRHINERLTAEVEKREHAQIAVHNLQDELVQASKLAVLGQIAASVAHEV
NQPVAAIRTFAENSQVLLIRGDTGTARINLSTIAGLTERIGTIMDELRAFSRKTTSEKEPISLRETIDGALLLVGHRLKQ
QTINLFVDLADADIVVAAERIRLEQVFVNLLQNAIEAVSNQPDGSIQILARPTAENVIVTIADNGPGFPDAVMTALFMPF
TTTKPDGVGLGLLISHDILNEFNGTLAVENCDGATLTITLPRVN

Sequences:

>Translated_604_residues
MGIARWHSMPCRLLLFALAAILILGFDGAARYVAQRSVLSELGAAAKIAASLRVAMLRNEIEKQRTLPIVLAQDPDVRAT
LERGGSVRLAALNAKLEALAAATRAGAIYLLDANGIAIAASNYRMPTSFVGTNYAFRPYFKRTLEEGSAEHFAFGTVSHK
PGLYLTQRLDGPTGPLGMLIVKAEFDAVEAEWRHGTAPTFVSDERGIILVTSEPSWRFRTKTPIPVERRAEILANLQFGD
AKLDLLPLHTNTGSIGLVEATLPGEYRPRYFVEGGSKVSSIDWTLHVLTPTETTMKLTVTAWRSLTLLLVTVALGVVGLW
LSRQHRLVEERKQHATARRELENMVEHRTKELRHINERLTAEVEKREHAQIAVHNLQDELVQASKLAVLGQIAASVAHEV
NQPVAAIRTFAENSQVLLIRGDTGTARINLSTIAGLTERIGTIMDELRAFSRKTTSEKEPISLRETIDGALLLVGHRLKQ
QTINLFVDLADADIVVAAERIRLEQVFVNLLQNAIEAVSNQPDGSIQILARPTAENVIVTIADNGPGFPDAVMTALFMPF
TTTKPDGVGLGLLISHDILNEFNGTLAVENCDGATLTITLPRVN
>Mature_603_residues
GIARWHSMPCRLLLFALAAILILGFDGAARYVAQRSVLSELGAAAKIAASLRVAMLRNEIEKQRTLPIVLAQDPDVRATL
ERGGSVRLAALNAKLEALAAATRAGAIYLLDANGIAIAASNYRMPTSFVGTNYAFRPYFKRTLEEGSAEHFAFGTVSHKP
GLYLTQRLDGPTGPLGMLIVKAEFDAVEAEWRHGTAPTFVSDERGIILVTSEPSWRFRTKTPIPVERRAEILANLQFGDA
KLDLLPLHTNTGSIGLVEATLPGEYRPRYFVEGGSKVSSIDWTLHVLTPTETTMKLTVTAWRSLTLLLVTVALGVVGLWL
SRQHRLVEERKQHATARRELENMVEHRTKELRHINERLTAEVEKREHAQIAVHNLQDELVQASKLAVLGQIAASVAHEVN
QPVAAIRTFAENSQVLLIRGDTGTARINLSTIAGLTERIGTIMDELRAFSRKTTSEKEPISLRETIDGALLLVGHRLKQQ
TINLFVDLADADIVVAAERIRLEQVFVNLLQNAIEAVSNQPDGSIQILARPTAENVIVTIADNGPGFPDAVMTALFMPFT
TTKPDGVGLGLLISHDILNEFNGTLAVENCDGATLTITLPRVN

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=33.0396475770925, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=31.4410480349345, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788393, Length=223, Percent_Identity=29.1479820627803, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1789149, Length=272, Percent_Identity=25.7352941176471, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=26.5232974910394, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66064; Mature: 65933

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIARWHSMPCRLLLFALAAILILGFDGAARYVAQRSVLSELGAAAKIAASLRVAMLRNE
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IEKQRTLPIVLAQDPDVRATLERGGSVRLAALNAKLEALAAATRAGAIYLLDANGIAIAA
HHHHCCCCEEEECCCCCHHHHHCCCCEEEEEECHHHHHHHHHHCCCEEEEEECCCEEEEE
SNYRMPTSFVGTNYAFRPYFKRTLEEGSAEHFAFGTVSHKPGLYLTQRLDGPTGPLGMLI
CCCCCCCHHCCCCCEECHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
VKAEFDAVEAEWRHGTAPTFVSDERGIILVTSEPSWRFRTKTPIPVERRAEILANLQFGD
EEECCCCHHHHCCCCCCCCEECCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHCCEECC
AKLDLLPLHTNTGSIGLVEATLPGEYRPRYFVEGGSKVSSIDWTLHVLTPTETTMKLTVT
CEEEEEEEECCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEEEEEEECCCCCEEEEEEH
AWRSLTLLLVTVALGVVGLWLSRQHRLVEERKQHATARRELENMVEHRTKELRHINERLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVEKREHAQIAVHNLQDELVQASKLAVLGQIAASVAHEVNQPVAAIRTFAENSQVLLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEE
GDTGTARINLSTIAGLTERIGTIMDELRAFSRKTTSEKEPISLRETIDGALLLVGHRLKQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEHHHHHHH
QTINLFVDLADADIVVAAERIRLEQVFVNLLQNAIEAVSNQPDGSIQILARPTAENVIVT
HEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
IADNGPGFPDAVMTALFMPFTTTKPDGVGLGLLISHDILNEFNGTLAVENCDGATLTITL
EECCCCCCHHHHHHHHHCCCCCCCCCCCCEEHEEEHHHHHHCCCEEEEECCCCCEEEEEE
PRVN
CCCC
>Mature Secondary Structure 
GIARWHSMPCRLLLFALAAILILGFDGAARYVAQRSVLSELGAAAKIAASLRVAMLRNE
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IEKQRTLPIVLAQDPDVRATLERGGSVRLAALNAKLEALAAATRAGAIYLLDANGIAIAA
HHHHCCCCEEEECCCCCHHHHHCCCCEEEEEECHHHHHHHHHHCCCEEEEEECCCEEEEE
SNYRMPTSFVGTNYAFRPYFKRTLEEGSAEHFAFGTVSHKPGLYLTQRLDGPTGPLGMLI
CCCCCCCHHCCCCCEECHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
VKAEFDAVEAEWRHGTAPTFVSDERGIILVTSEPSWRFRTKTPIPVERRAEILANLQFGD
EEECCCCHHHHCCCCCCCCEECCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHCCEECC
AKLDLLPLHTNTGSIGLVEATLPGEYRPRYFVEGGSKVSSIDWTLHVLTPTETTMKLTVT
CEEEEEEEECCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEEEEEEECCCCCEEEEEEH
AWRSLTLLLVTVALGVVGLWLSRQHRLVEERKQHATARRELENMVEHRTKELRHINERLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVEKREHAQIAVHNLQDELVQASKLAVLGQIAASVAHEVNQPVAAIRTFAENSQVLLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEE
GDTGTARINLSTIAGLTERIGTIMDELRAFSRKTTSEKEPISLRETIDGALLLVGHRLKQ
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEHHHHHHH
QTINLFVDLADADIVVAAERIRLEQVFVNLLQNAIEAVSNQPDGSIQILARPTAENVIVT
HEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
IADNGPGFPDAVMTALFMPFTTTKPDGVGLGLLISHDILNEFNGTLAVENCDGATLTITL
EECCCCCCHHHHHHHHHCCCCCCCCCCCCEEHEEEHHHHHHCCCEEEEECCCCCEEEEEE
PRVN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]