Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 182677125

GI number: 182677125

Start: 144522

End: 146099

Strand: Direct

Name: oprM [H]

Synonym: Bind_0124

Alternate gene names: 182677125

Gene position: 144522-146099 (Clockwise)

Preceding gene: 182677124

Following gene: 182677126

Centisome position: 3.47

GC content: 57.86

Gene sequence:

>1578_bases
ATGAAATGCCTCTTCCCAGCAGGAGCCCGTTCCAGTGGTCTTCTGTTTCTTTTGTCCCTCTCCGCCTGTGATCTCGCCCC
CGCCTACCAGCCGCCAGACCTCGAACTTCCCACCCATTGGCAAGGCAAGGGTCCCTTCAAGGAGGCCCAGCCCGCCGATA
CAGCCGTGCGGCCAGGCTGGTGGAAGCTCTATCACGATCCTCTCCTCAACCGAATCGAGGAAGAGGCGACGGCAAGCAAT
GCTGATCTGCAAGCTGCAGCTGAGCGATTCGTTCAGGCGCGTGACATGATCATGGGGGCGCAATCGCAATTGTTCCCGCA
AGTCGGGATTGGTTTCGGCGCCTCCAACAACAAGCGTTCGGTCCATCGCCTGTTCAACTCGCCGGCCGATCCGATCCATG
ATTCCGATGAGACATTCGCGGGCCTCGTCTCCTGGGAGCCGGATATCTGGTCGGCGATCCGCAACAAGACTCGCGCGGAA
TTGAATTTCGCGGAACAGCGCGCGGCTGAATATGCTTCGGCGCAATTGAGTCTGCAGTCACAGATCGCTTGGGATTATTT
CGCGCTGCGTGGTCTCGACGCGCAGGATGCGATCTATAAACAATCGATCGCCTATTATCAGCAGTCGCTCAACATTATCA
AAGAGCGTCTCTCGGGCCTCGTCTCGCCGAAGCTCGATGTCGCGCGCGCTGAGAACCTGCTTTATAGCACGCAGGCGGAA
GAGCTCGATATTCAGGCGCAGCGTCAGGTCATGGAACATGCGATCGCCATTCTGGCGAATACGGCGCCAAGCGCTTTCCA
TATTCCACCCGCCAATACGTTGCACACATCAATCCCCAAAATCCCGACGCGTGTCCCTTCCGATCTTCTGCAACGCCGGC
CCGATATCGCCAGCGCGGAACGCAAGATGGCGCAGGCCAATCGCATCATCGGCATTGCGCGGGCGGCCTTCTATCCGAAT
GTCTCCATCCGCGTCGATGGTGGCTTCACCGATTCCGGTCTCAATCTCTTCAAGGTGGCCAACAGCCTCTGGGCTTATGG
CGCTGATGTCACCCTGCCGATTTTCGATGGCGGATTACGCCGCGCCGATCTGCAGCAATCTCTCTCCGCTTATCGCGAAA
CCACGGATAATTACCGCTCCACGGTCCTCAATGCCTTTCGCGAGGTGGAAGATGGTCTGAGCCGCACCAATCGGCTGAGC
GCGGAAGTCAAGAAGCAGCATGCCGCCGTCACCGCTGCCTCGGAGACGCAGACCATGACCATGGATCTCTATAAAGGCGG
TCTGAATACCAGTCTCGATGTCATCACCGCCCAGGTGAATACATTGACGGCGCGTATTCGCGAAGTGCAGATTCAAATGC
AATTGCTGCAGGTCTCGACCGAATTGATCCGTGCGCTCGGCGGCGGCTGGGACAAGAGCAACTTGCCCGAGCAGCGGGAA
ATGCAGGCTTTTGGCATCTTCCAGGTCGATAATCTGGATAAGCCGCCGCCGGCCGGTGGCATTGACGTGCAGACCGATCC
TCAGGCCAATAGTGATTTGACCCGACCAATTTCGCAACAAAGCAAGGCTGGGCACTGA

Upstream 100 bases:

>100_bases
ACAATAATGCGGTTCCAAACCATGAGGCGACGCAGGCCAATGGGTCCCGTCAGCAGCTTGGTCTGGCGGATGAACAGACT
TCGGCGCAAGAGACCAAAAA

Downstream 100 bases:

>100_bases
GTATCAGCGGCTCCGAAAAGAACATTGCAGAGCGTGAGGGCTGTATGCGCGCACCTCTCGATATAATGCAATTACATCGA
GAGGCATGTCTCGGTGTATT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKCLFPAGARSSGLLFLLSLSACDLAPAYQPPDLELPTHWQGKGPFKEAQPADTAVRPGWWKLYHDPLLNRIEEEATASN
ADLQAAAERFVQARDMIMGAQSQLFPQVGIGFGASNNKRSVHRLFNSPADPIHDSDETFAGLVSWEPDIWSAIRNKTRAE
LNFAEQRAAEYASAQLSLQSQIAWDYFALRGLDAQDAIYKQSIAYYQQSLNIIKERLSGLVSPKLDVARAENLLYSTQAE
ELDIQAQRQVMEHAIAILANTAPSAFHIPPANTLHTSIPKIPTRVPSDLLQRRPDIASAERKMAQANRIIGIARAAFYPN
VSIRVDGGFTDSGLNLFKVANSLWAYGADVTLPIFDGGLRRADLQQSLSAYRETTDNYRSTVLNAFREVEDGLSRTNRLS
AEVKKQHAAVTAASETQTMTMDLYKGGLNTSLDVITAQVNTLTARIREVQIQMQLLQVSTELIRALGGGWDKSNLPEQRE
MQAFGIFQVDNLDKPPPAGGIDVQTDPQANSDLTRPISQQSKAGH

Sequences:

>Translated_525_residues
MKCLFPAGARSSGLLFLLSLSACDLAPAYQPPDLELPTHWQGKGPFKEAQPADTAVRPGWWKLYHDPLLNRIEEEATASN
ADLQAAAERFVQARDMIMGAQSQLFPQVGIGFGASNNKRSVHRLFNSPADPIHDSDETFAGLVSWEPDIWSAIRNKTRAE
LNFAEQRAAEYASAQLSLQSQIAWDYFALRGLDAQDAIYKQSIAYYQQSLNIIKERLSGLVSPKLDVARAENLLYSTQAE
ELDIQAQRQVMEHAIAILANTAPSAFHIPPANTLHTSIPKIPTRVPSDLLQRRPDIASAERKMAQANRIIGIARAAFYPN
VSIRVDGGFTDSGLNLFKVANSLWAYGADVTLPIFDGGLRRADLQQSLSAYRETTDNYRSTVLNAFREVEDGLSRTNRLS
AEVKKQHAAVTAASETQTMTMDLYKGGLNTSLDVITAQVNTLTARIREVQIQMQLLQVSTELIRALGGGWDKSNLPEQRE
MQAFGIFQVDNLDKPPPAGGIDVQTDPQANSDLTRPISQQSKAGH
>Mature_525_residues
MKCLFPAGARSSGLLFLLSLSACDLAPAYQPPDLELPTHWQGKGPFKEAQPADTAVRPGWWKLYHDPLLNRIEEEATASN
ADLQAAAERFVQARDMIMGAQSQLFPQVGIGFGASNNKRSVHRLFNSPADPIHDSDETFAGLVSWEPDIWSAIRNKTRAE
LNFAEQRAAEYASAQLSLQSQIAWDYFALRGLDAQDAIYKQSIAYYQQSLNIIKERLSGLVSPKLDVARAENLLYSTQAE
ELDIQAQRQVMEHAIAILANTAPSAFHIPPANTLHTSIPKIPTRVPSDLLQRRPDIASAERKMAQANRIIGIARAAFYPN
VSIRVDGGFTDSGLNLFKVANSLWAYGADVTLPIFDGGLRRADLQQSLSAYRETTDNYRSTVLNAFREVEDGLSRTNRLS
AEVKKQHAAVTAASETQTMTMDLYKGGLNTSLDVITAQVNTLTARIREVQIQMQLLQVSTELIRALGGGWDKSNLPEQRE
MQAFGIFQVDNLDKPPPAGGIDVQTDPQANSDLTRPISQQSKAGH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=27.2138228941685, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI2367348, Length=441, Percent_Identity=24.7165532879819, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57855; Mature: 57855

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCLFPAGARSSGLLFLLSLSACDLAPAYQPPDLELPTHWQGKGPFKEAQPADTAVRPGW
CCCCCCCCCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
WKLYHDPLLNRIEEEATASNADLQAAAERFVQARDMIMGAQSQLFPQVGIGFGASNNKRS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCHHHCCCCCCCCCHHH
VHRLFNSPADPIHDSDETFAGLVSWEPDIWSAIRNKTRAELNFAEQRAAEYASAQLSLQS
HHHHHCCCCCCCCCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIAWDYFALRGLDAQDAIYKQSIAYYQQSLNIIKERLSGLVSPKLDVARAENLLYSTQAE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
ELDIQAQRQVMEHAIAILANTAPSAFHIPPANTLHTSIPKIPTRVPSDLLQRRPDIASAE
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCCCCHHHHHCCCCHHHHH
RKMAQANRIIGIARAAFYPNVSIRVDGGFTDSGLNLFKVANSLWAYGADVTLPIFDGGLR
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
RADLQQSLSAYRETTDNYRSTVLNAFREVEDGLSRTNRLSAEVKKQHAAVTAASETQTMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEE
MDLYKGGLNTSLDVITAQVNTLTARIREVQIQMQLLQVSTELIRALGGGWDKSNLPEQRE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
MQAFGIFQVDNLDKPPPAGGIDVQTDPQANSDLTRPISQQSKAGH
HHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MKCLFPAGARSSGLLFLLSLSACDLAPAYQPPDLELPTHWQGKGPFKEAQPADTAVRPGW
CCCCCCCCCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
WKLYHDPLLNRIEEEATASNADLQAAAERFVQARDMIMGAQSQLFPQVGIGFGASNNKRS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCHHHCCCCCCCCCHHH
VHRLFNSPADPIHDSDETFAGLVSWEPDIWSAIRNKTRAELNFAEQRAAEYASAQLSLQS
HHHHHCCCCCCCCCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIAWDYFALRGLDAQDAIYKQSIAYYQQSLNIIKERLSGLVSPKLDVARAENLLYSTQAE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
ELDIQAQRQVMEHAIAILANTAPSAFHIPPANTLHTSIPKIPTRVPSDLLQRRPDIASAE
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCCCCHHHHHCCCCHHHHH
RKMAQANRIIGIARAAFYPNVSIRVDGGFTDSGLNLFKVANSLWAYGADVTLPIFDGGLR
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
RADLQQSLSAYRETTDNYRSTVLNAFREVEDGLSRTNRLSAEVKKQHAAVTAASETQTMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEE
MDLYKGGLNTSLDVITAQVNTLTARIREVQIQMQLLQVSTELIRALGGGWDKSNLPEQRE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
MQAFGIFQVDNLDKPPPAGGIDVQTDPQANSDLTRPISQQSKAGH
HHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]