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The map label for this gene is thiD [H]

Identifier: 182677126

GI number: 182677126

Start: 147095

End: 147952

Strand: Direct

Name: thiD [H]

Synonym: Bind_0125

Alternate gene names: 182677126

Gene position: 147095-147952 (Clockwise)

Preceding gene: 182677125

Following gene: 182677129

Centisome position: 3.53

GC content: 61.77

Gene sequence:

>858_bases
ATGAACGATTCGCTGAGAGGGGGAGCGGAGAGAGCGGCGCTTATCCCGAATGTCCTTTCTATTGCTGGATCGGATCCGAG
CGGGGGGGCGGGCATTCAGGCCGATCTCAAAACATTCGCGGCGCTCGAATGTCATGGCATGGCGGCCATCACGGCTCTGA
CCGTTCAGAATACGCAAGGGGTCCGCGCGATACATGTGCCTCCGGCCGATTTCGTCGGTGCGGAGATTGACGCGCTTTTC
GCCGACAGCACTATTGCCGCCGTGAAGATTGGCATGTTGGCCGAGGCTTCGATCGCCAAAGCTGTGGCCGCAAGCCTGGC
GCGCCACGCGCCGCCCCACATCATTCTCGATCCCGTGCTGGCCGCAACGAGCGGCGATTCGCTCTCCGGCCCCGCTCTCG
CTGAAGCGCTCGTCACCCATCTTTTTCCGCTTGCGACGCTCGTCACGCCCAATCTGAGCGAGGCTGCGCACCTGACGGGT
CTGCCCTTTTCCGCGGCAAAGGATAGTATGGGGGTAACAGCCGAGGCGCTTATGGCATGCGGTGCGCGCGCCGTACTCCT
CAAAGGGGGCCATCTCGAAGGGGATCTCGCGGAGGATCTTTTATACACCGCCTCGTATCAGGAAAGGTTCAGCGCGCGGC
GCGTCGAAACACGCAATACACATGGAACCGGTTGTACATTGGCTTCAGCCATCGCCGCCTTTCTCGCCCATGGCCACGAT
CTTCGTTCCGCTGTAGCGGCGGCGAAAGCCTATCTGACGCGGGCATTGGAATCGGCCGACAGGCTCGATGTTGGCCAAGG
CCACGGGCCGGTTCATCATTTTCATGCTCTCTGGCCATCCCGCAGCGGGGGAGTCTGA

Upstream 100 bases:

>100_bases
AAGCTTCTTTGAGAGAATGAGTGTCCTGTTATAAAATTATAAGAGCTTACGCTCTTGAGCTCGAATCAGCTTGGTTCCGA
AACAGGCCCGAAAGGACGAT

Downstream 100 bases:

>100_bases
CGTCTCAGCGCGGCACGAGATCCCTCAAAGCGCGCTCAGCCGCGCGATAGTGCGCGTCAACAAGGGCAAGGCGCTGCCGG
CAAACTCGCGCAGACCGGGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNDSLRGGAERAALIPNVLSIAGSDPSGGAGIQADLKTFAALECHGMAAITALTVQNTQGVRAIHVPPADFVGAEIDALF
ADSTIAAVKIGMLAEASIAKAVAASLARHAPPHIILDPVLAATSGDSLSGPALAEALVTHLFPLATLVTPNLSEAAHLTG
LPFSAAKDSMGVTAEALMACGARAVLLKGGHLEGDLAEDLLYTASYQERFSARRVETRNTHGTGCTLASAIAAFLAHGHD
LRSAVAAAKAYLTRALESADRLDVGQGHGPVHHFHALWPSRSGGV

Sequences:

>Translated_285_residues
MNDSLRGGAERAALIPNVLSIAGSDPSGGAGIQADLKTFAALECHGMAAITALTVQNTQGVRAIHVPPADFVGAEIDALF
ADSTIAAVKIGMLAEASIAKAVAASLARHAPPHIILDPVLAATSGDSLSGPALAEALVTHLFPLATLVTPNLSEAAHLTG
LPFSAAKDSMGVTAEALMACGARAVLLKGGHLEGDLAEDLLYTASYQERFSARRVETRNTHGTGCTLASAIAAFLAHGHD
LRSAVAAAKAYLTRALESADRLDVGQGHGPVHHFHALWPSRSGGV
>Mature_285_residues
MNDSLRGGAERAALIPNVLSIAGSDPSGGAGIQADLKTFAALECHGMAAITALTVQNTQGVRAIHVPPADFVGAEIDALF
ADSTIAAVKIGMLAEASIAKAVAASLARHAPPHIILDPVLAATSGDSLSGPALAEALVTHLFPLATLVTPNLSEAAHLTG
LPFSAAKDSMGVTAEALMACGARAVLLKGGHLEGDLAEDLLYTASYQERFSARRVETRNTHGTGCTLASAIAAFLAHGHD
LRSAVAAAKAYLTRALESADRLDVGQGHGPVHHFHALWPSRSGGV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=48.8549618320611, Blast_Score=253, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6325378, Length=275, Percent_Identity=33.8181818181818, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=278, Percent_Identity=37.7697841726619, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=285, Percent_Identity=36.4912280701754, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29076; Mature: 29076

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSLRGGAERAALIPNVLSIAGSDPSGGAGIQADLKTFAALECHGMAAITALTVQNTQG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
VRAIHVPPADFVGAEIDALFADSTIAAVKIGMLAEASIAKAVAASLARHAPPHIILDPVL
EEEEECCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHH
AATSGDSLSGPALAEALVTHLFPLATLVTPNLSEAAHLTGLPFSAAKDSMGVTAEALMAC
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCCCCCCHHHHHHHHC
GARAVLLKGGHLEGDLAEDLLYTASYQERFSARRVETRNTHGTGCTLASAIAAFLAHGHD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHH
LRSAVAAAKAYLTRALESADRLDVGQGHGPVHHFHALWPSRSGGV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDSLRGGAERAALIPNVLSIAGSDPSGGAGIQADLKTFAALECHGMAAITALTVQNTQG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
VRAIHVPPADFVGAEIDALFADSTIAAVKIGMLAEASIAKAVAASLARHAPPHIILDPVL
EEEEECCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHH
AATSGDSLSGPALAEALVTHLFPLATLVTPNLSEAAHLTGLPFSAAKDSMGVTAEALMAC
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCCCCCCHHHHHHHHC
GARAVLLKGGHLEGDLAEDLLYTASYQERFSARRVETRNTHGTGCTLASAIAAFLAHGHD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHH
LRSAVAAAKAYLTRALESADRLDVGQGHGPVHHFHALWPSRSGGV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]