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The map label for this gene is glcF [H]

Identifier: 17547387

GI number: 17547387

Start: 2878363

End: 2879610

Strand: Direct

Name: glcF [H]

Synonym: RSc2668

Alternate gene names: 17547387

Gene position: 2878363-2879610 (Clockwise)

Preceding gene: 17547386

Following gene: 17547388

Centisome position: 77.45

GC content: 70.27

Gene sequence:

>1248_bases
ATGCAAATCACGCTCGCGGCGTTCCTGCGGAACACCCCCGACGGCGAAGAGGCCCAGGGCATCGTGCAGAAGTGCGTGCA
CTGCGGCTTCTGCACGGCCACCTGCCCGACCTACCAGTTGCTCGGCGACGAGCTCGACGGACCGCGCGGCCGCATCTACC
TGATGAAGCAAGTGCTGGAAGGCCAGCCGGCCGGCGAGCGCACCCGCCTGCACCTGGACCGCTGCCTGACCTGCCGCAAC
TGCGAATCGACCTGCCCATCGGGCGTGACCTACGGCCGGCTGGTCGAGATCGGCCGCAAGATCGTCGACGACCAGCTCGA
CGCGCAGGGCGCATCGCGCCCGCTGGGCGAGCGCGCGATGCGTTGGGTGCTGCGCGAGGGGCTGACGCGGCCGCGGCTGT
TCGGCCCCGCGCTCAGGCTGGGCCAGGCCGTGCGCCCGCTGCTGCCAAGCGTGCTGCGCAACAAGGTGCCGGCCAGGGCG
CCGCAGGCCGGTACGCGGCCCACCGCCACCCATGCGCGCAAGATGCTGCTGCTGGAAGGCTGCGTGCAGCCGGCCATGTC
GCCCAACATCAATGCCGCCGCCGCGCGCGTCTTCGACAGGCTGGGCGTGCAGTTGGTCAGCGCGGACCGTGCGGGCTGCT
GCGGCGCGATCCGCTATCACATGAACGACCACGCGGGCGGCCTCGGCAACATGCGCGCCAACATCGACGCCTGGTGGCCG
CACATCGAGGCCGGCGCCGAGGCCATCGTCATGACGGCATCCGGCTGCGGCGCGATGGTCAAGGAATACGGCCACCTGCT
GCGCGACGACCCGGACTATGCCGAACGGGCGGCGCGCGTGTCGGCCATGACGCGCGATCTGTGCGAGGTGCTGCCCGCCT
TCTCCGACCAGCTGCTGCGGCAGGCCAAACCCGAAGCCCACCGACCGCGCGTGGCCTGGCACGCGCCTTGCACGCTGCAG
CACGGCCAGCAGATCCGCGGCGCGGTGGAAGGCCTGCTCGCATTATTGGGCGTGGAGGTGCGGCTCTGCACCGACAGCCA
CCTGTGCTGCGGATCGGCGGGCACCTATTCGGTGCTGCAGCCCGGACTGGCGTATCGCCTGCGCGACGACAAGCTGCGCC
GCTTGCTTGCCACGCAGCCGGAACGTATCGTGTCGGCCAACATCGGCTGCGTGACGCATCTGCAAAGCGGCACCGATACG
CCGGTCGAGCATTGGATCGAACTGGTGGACCGCATGCTGGCCGGATGA

Upstream 100 bases:

>100_bases
CGCGCCGCTAATGGCGGTGCACAAGCGCCTGAAGGACAGCTTCGACCCGCACGGCATCTTCAACCCCGGCCGCCTGTACG
CCGATTTCTGACCCGCCATC

Downstream 100 bases:

>100_bases
CCGGACCGTGCATCGCGCGGCCGATCGACGCTTGTCAATTTTGGAGGCCGCGTGCTGCAAACCTTCGCCATCCTGCTGAC
CTTCCAGTCGGTGGGTGAGC

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQITLAAFLRNTPDGEEAQGIVQKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGQPAGERTRLHLDRCLTCRN
CESTCPSGVTYGRLVEIGRKIVDDQLDAQGASRPLGERAMRWVLREGLTRPRLFGPALRLGQAVRPLLPSVLRNKVPARA
PQAGTRPTATHARKMLLLEGCVQPAMSPNINAAAARVFDRLGVQLVSADRAGCCGAIRYHMNDHAGGLGNMRANIDAWWP
HIEAGAEAIVMTASGCGAMVKEYGHLLRDDPDYAERAARVSAMTRDLCEVLPAFSDQLLRQAKPEAHRPRVAWHAPCTLQ
HGQQIRGAVEGLLALLGVEVRLCTDSHLCCGSAGTYSVLQPGLAYRLRDDKLRRLLATQPERIVSANIGCVTHLQSGTDT
PVEHWIELVDRMLAG

Sequences:

>Translated_415_residues
MQITLAAFLRNTPDGEEAQGIVQKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGQPAGERTRLHLDRCLTCRN
CESTCPSGVTYGRLVEIGRKIVDDQLDAQGASRPLGERAMRWVLREGLTRPRLFGPALRLGQAVRPLLPSVLRNKVPARA
PQAGTRPTATHARKMLLLEGCVQPAMSPNINAAAARVFDRLGVQLVSADRAGCCGAIRYHMNDHAGGLGNMRANIDAWWP
HIEAGAEAIVMTASGCGAMVKEYGHLLRDDPDYAERAARVSAMTRDLCEVLPAFSDQLLRQAKPEAHRPRVAWHAPCTLQ
HGQQIRGAVEGLLALLGVEVRLCTDSHLCCGSAGTYSVLQPGLAYRLRDDKLRRLLATQPERIVSANIGCVTHLQSGTDT
PVEHWIELVDRMLAG
>Mature_415_residues
MQITLAAFLRNTPDGEEAQGIVQKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGQPAGERTRLHLDRCLTCRN
CESTCPSGVTYGRLVEIGRKIVDDQLDAQGASRPLGERAMRWVLREGLTRPRLFGPALRLGQAVRPLLPSVLRNKVPARA
PQAGTRPTATHARKMLLLEGCVQPAMSPNINAAAARVFDRLGVQLVSADRAGCCGAIRYHMNDHAGGLGNMRANIDAWWP
HIEAGAEAIVMTASGCGAMVKEYGHLLRDDPDYAERAARVSAMTRDLCEVLPAFSDQLLRQAKPEAHRPRVAWHAPCTLQ
HGQQIRGAVEGLLALLGVEVRLCTDSHLCCGSAGTYSVLQPGLAYRLRDDKLRRLLATQPERIVSANIGCVTHLQSGTDT
PVEHWIELVDRMLAG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=413, Percent_Identity=53.9951573849879, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1786497, Length=238, Percent_Identity=23.9495798319328, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 45309; Mature: 45309

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITLAAFLRNTPDGEEAQGIVQKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
CEEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GQPAGERTRLHLDRCLTCRNCESTCPSGVTYGRLVEIGRKIVDDQLDAQGASRPLGERAM
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RWVLREGLTRPRLFGPALRLGQAVRPLLPSVLRNKVPARAPQAGTRPTATHARKMLLLEG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
CVQPAMSPNINAAAARVFDRLGVQLVSADRAGCCGAIRYHMNDHAGGLGNMRANIDAWWP
HHHHCCCCCCHHHHHHHHHHHCCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHCC
HIEAGAEAIVMTASGCGAMVKEYGHLLRDDPDYAERAARVSAMTRDLCEVLPAFSDQLLR
CHHCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKPEAHRPRVAWHAPCTLQHGQQIRGAVEGLLALLGVEVRLCTDSHLCCGSAGTYSVLQ
HCCCCCCCCCEEEECCCCHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHC
PGLAYRLRDDKLRRLLATQPERIVSANIGCVTHLQSGTDTPVEHWIELVDRMLAG
CCHHHHCCHHHHHHHHHCCCHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQITLAAFLRNTPDGEEAQGIVQKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
CEEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GQPAGERTRLHLDRCLTCRNCESTCPSGVTYGRLVEIGRKIVDDQLDAQGASRPLGERAM
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RWVLREGLTRPRLFGPALRLGQAVRPLLPSVLRNKVPARAPQAGTRPTATHARKMLLLEG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
CVQPAMSPNINAAAARVFDRLGVQLVSADRAGCCGAIRYHMNDHAGGLGNMRANIDAWWP
HHHHCCCCCCHHHHHHHHHHHCCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHCC
HIEAGAEAIVMTASGCGAMVKEYGHLLRDDPDYAERAARVSAMTRDLCEVLPAFSDQLLR
CHHCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKPEAHRPRVAWHAPCTLQHGQQIRGAVEGLLALLGVEVRLCTDSHLCCGSAGTYSVLQ
HCCCCCCCCCEEEECCCCHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHC
PGLAYRLRDDKLRRLLATQPERIVSANIGCVTHLQSGTDTPVEHWIELVDRMLAG
CCHHHHCCHHHHHHHHHCCCHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]