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The map label for this gene is glcE [H]

Identifier: 17547386

GI number: 17547386

Start: 2877235

End: 2878353

Strand: Direct

Name: glcE [H]

Synonym: RSc2667

Alternate gene names: 17547386

Gene position: 2877235-2878353 (Clockwise)

Preceding gene: 17547385

Following gene: 17547387

Centisome position: 77.42

GC content: 73.82

Gene sequence:

>1119_bases
ATGACCGCACTCGACCCCGCGCTCGCGCGCCTGCGCGACGCCATCCTGCAGGCCGCCGCCGACCGCGCGCCGCTCACCCT
GCGCGGCGGCGGCACCAAGGATTTCTACGGCCGCAAACCCGCCCCCGGCGCCACCGTGCTCGACACGCGCGAGTGGTCCG
GCATCGTCGACTATGACTGCGCGGAACTCGTCGTCACGGCGCGCTGCGGCACGCCGCTGGCCGAGGTGGAAGCCGCGCTG
GCCGAGCACCGCCAGATGCTGGCCTTCGAGCCGCCGCATTTCGCCGCCGGCGGCGCGCGGGCGACGGTGGGCGGCGCCTT
CGCGTGCGGCCTGTCCGGCCCGCGCCGGCAGTCGGTGGGCGCGCTGCGCGATTTCGTGCTCGGCGCCGTGATCATGGACG
GCCGCGGCGACCTGCTCAACTTCGGCGGCCAGGTGATGAAGAACGTGGCCGGCTATGACGTGTCGCGCCTGATGGCCGGC
GCGCTGGGCACGCTGGGGCTGGTGGCGCAGGTCTCGCTGAAGGTGCTGCCGATGCCGTTCTGCGATGCCACGCTGCGCCT
GGCCATGCCGCAGGCCGAGGCCATCGATACGCTCAACCGCTGGGGCGGCCAGCCGCTGCCGATCGCGGCGTCGGCCTGGA
TCGACGGCACACTGTGGGTCCGCCTGTGCGGCGCCGATGCCGCCGTGCGCGCAGCCCGCGCCAGGCTCGGCGGCGACCGG
GTCGACGAGGCCGAAGCACTGGCGCTGTGGCGTGACCTGCGCGAGCAGTCGCACGCGTTCTTCGCACCCGCCGTGCAGGG
CAGCCTGCCGCTATGGCGCATCGCCCTGCCTCCGGCCACGCCGCCGCTGGCATTGGGCCCTGCGGCGGCCGACGACCAGC
TCATCGAATGGGGCGGCGGCCAGCGGTGGTGGATCGACACCGGCAACACCCCTGCCGACGCCATCCGCGATGCCGTGCGC
CGGGCCGGCGGCCATGCCACGCTGTTCCGCAACGGCGACCGCGACAGTGCCGTCTTCACCCCCGTCGCCGCGCCGCTAAT
GGCGGTGCACAAGCGCCTGAAGGACAGCTTCGACCCGCACGGCATCTTCAACCCCGGCCGCCTGTACGCCGATTTCTGA

Upstream 100 bases:

>100_bases
GCCATCCCCACGCTCGCGCGCTGCGCCGAATACGGCAAGCTGCACGTGCGCAACGGCCTGCTGCCGCACCCCGACCTGCC
GCGCTTCTGAGGCCGCACGC

Downstream 100 bases:

>100_bases
CCCGCCATCATGCAAATCACGCTCGCGGCGTTCCTGCGGAACACCCCCGACGGCGAAGAGGCCCAGGGCATCGTGCAGAA
GTGCGTGCACTGCGGCTTCT

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTALDPALARLRDAILQAAADRAPLTLRGGGTKDFYGRKPAPGATVLDTREWSGIVDYDCAELVVTARCGTPLAEVEAAL
AEHRQMLAFEPPHFAAGGARATVGGAFACGLSGPRRQSVGALRDFVLGAVIMDGRGDLLNFGGQVMKNVAGYDVSRLMAG
ALGTLGLVAQVSLKVLPMPFCDATLRLAMPQAEAIDTLNRWGGQPLPIAASAWIDGTLWVRLCGADAAVRAARARLGGDR
VDEAEALALWRDLREQSHAFFAPAVQGSLPLWRIALPPATPPLALGPAAADDQLIEWGGGQRWWIDTGNTPADAIRDAVR
RAGGHATLFRNGDRDSAVFTPVAAPLMAVHKRLKDSFDPHGIFNPGRLYADF

Sequences:

>Translated_372_residues
MTALDPALARLRDAILQAAADRAPLTLRGGGTKDFYGRKPAPGATVLDTREWSGIVDYDCAELVVTARCGTPLAEVEAAL
AEHRQMLAFEPPHFAAGGARATVGGAFACGLSGPRRQSVGALRDFVLGAVIMDGRGDLLNFGGQVMKNVAGYDVSRLMAG
ALGTLGLVAQVSLKVLPMPFCDATLRLAMPQAEAIDTLNRWGGQPLPIAASAWIDGTLWVRLCGADAAVRAARARLGGDR
VDEAEALALWRDLREQSHAFFAPAVQGSLPLWRIALPPATPPLALGPAAADDQLIEWGGGQRWWIDTGNTPADAIRDAVR
RAGGHATLFRNGDRDSAVFTPVAAPLMAVHKRLKDSFDPHGIFNPGRLYADF
>Mature_371_residues
TALDPALARLRDAILQAAADRAPLTLRGGGTKDFYGRKPAPGATVLDTREWSGIVDYDCAELVVTARCGTPLAEVEAALA
EHRQMLAFEPPHFAAGGARATVGGAFACGLSGPRRQSVGALRDFVLGAVIMDGRGDLLNFGGQVMKNVAGYDVSRLMAGA
LGTLGLVAQVSLKVLPMPFCDATLRLAMPQAEAIDTLNRWGGQPLPIAASAWIDGTLWVRLCGADAAVRAARARLGGDRV
DEAEALALWRDLREQSHAFFAPAVQGSLPLWRIALPPATPPLALGPAAADDQLIEWGGGQRWWIDTGNTPADAIRDAVRR
AGGHATLFRNGDRDSAVFTPVAAPLMAVHKRLKDSFDPHGIFNPGRLYADF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=364, Percent_Identity=47.8021978021978, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 39426; Mature: 39294

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALDPALARLRDAILQAAADRAPLTLRGGGTKDFYGRKPAPGATVLDTREWSGIVDYDC
CCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCH
AELVVTARCGTPLAEVEAALAEHRQMLAFEPPHFAAGGARATVGGAFACGLSGPRRQSVG
HHHEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHH
ALRDFVLGAVIMDGRGDLLNFGGQVMKNVAGYDVSRLMAGALGTLGLVAQVSLKVLPMPF
HHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCC
CDATLRLAMPQAEAIDTLNRWGGQPLPIAASAWIDGTLWVRLCGADAAVRAARARLGGDR
CCCHHHHCCCCHHHHHHHHHCCCCCCCEEHHHHCCCEEEEEEECCHHHHHHHHHHHCCCC
VDEAEALALWRDLREQSHAFFAPAVQGSLPLWRIALPPATPPLALGPAAADDQLIEWGGG
CCHHHHHHHHHHHHHHCCEEECHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECCCC
QRWWIDTGNTPADAIRDAVRRAGGHATLFRNGDRDSAVFTPVAAPLMAVHKRLKDSFDPH
CEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEECHHHHHHHHHHHHHHHCCCCC
GIFNPGRLYADF
CCCCCCCEEECC
>Mature Secondary Structure 
TALDPALARLRDAILQAAADRAPLTLRGGGTKDFYGRKPAPGATVLDTREWSGIVDYDC
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCH
AELVVTARCGTPLAEVEAALAEHRQMLAFEPPHFAAGGARATVGGAFACGLSGPRRQSVG
HHHEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHH
ALRDFVLGAVIMDGRGDLLNFGGQVMKNVAGYDVSRLMAGALGTLGLVAQVSLKVLPMPF
HHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCC
CDATLRLAMPQAEAIDTLNRWGGQPLPIAASAWIDGTLWVRLCGADAAVRAARARLGGDR
CCCHHHHCCCCHHHHHHHHHCCCCCCCEEHHHHCCCEEEEEEECCHHHHHHHHHHHCCCC
VDEAEALALWRDLREQSHAFFAPAVQGSLPLWRIALPPATPPLALGPAAADDQLIEWGGG
CCHHHHHHHHHHHHHHCCEEECHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECCCC
QRWWIDTGNTPADAIRDAVRRAGGHATLFRNGDRDSAVFTPVAAPLMAVHKRLKDSFDPH
CEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEECHHHHHHHHHHHHHHHCCCCC
GIFNPGRLYADF
CCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]