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The map label for this gene is glcD [H]

Identifier: 17547385

GI number: 17547385

Start: 2875731

End: 2877224

Strand: Direct

Name: glcD [H]

Synonym: RSc2666

Alternate gene names: 17547385

Gene position: 2875731-2877224 (Clockwise)

Preceding gene: 17547384

Following gene: 17547386

Centisome position: 77.38

GC content: 70.15

Gene sequence:

>1494_bases
ATGAACGCGCCGCTGCCCGCCGCCGCCCGCGATCCGTCTGCCGTGCAGGCCGAGCTGATCGCCGCGCTGTCGCCCCTGAT
GCCCGCCGATGCCCTGCTGTGGGAGCGCGAAGACACCACCCCCTACGAATGCGACGGTCTCGCCGCCTACCGGCAGGTGC
CGCTGGCCGTGGCGCTGCCGGACAGCGAAGCGCAGGTCGTGGAGATCCTGCGCGCCTGCCACCGCCTGGGCGTGCCGGTG
GTGCCGCGCGGCGCCGGCACCAGCCTGTCGGGCGGCGCGATGCCGACGCCGGGCGGACTGGTGCTGTCGCTGGCCAAGTT
CAAGCGCATCCTGAAGCTCGACCCGCACACCCGCACCGCGGCCGTGCAGCCGGGCGTGCGCAACCTGGCCATCTCGGAGG
CCGCCGCGCCGCACGGGCTGTACTACGCGCCCGATCCGTCGTCGCAGATCGCCTGCACCATCGGCGGCAACGTCAGCGAG
AACTCCGGCGGCGTGCATTGCCTGAAGTACGGCCTGACCGTGCACAACGTGCTGCGCGTGCGCGCCGTGATGATGGACGG
CGAGGTGGTCGAGTTCGGCAGCGAGGCACCCGACGCGCCCGGCCTGGACCTGCTCGCCGCCGTGATCGGCTCCGAAGGCA
TGCTGGCCGTGGTGACGGAGGTGACCGTGCGGCTGGTGCCCAAGCCGCAGCTGGCGCAGGTCATCATGGCGAGCTTCGAT
GACGTGGAAGCGGGCGGCAATGCGGTGGCCGAGGTGATCGCCGCCGGCATCATCCCGGCCGGGCTGGAGATGATGGACAA
GCCCGCCACCGCCGCCGTCGAGGAATTCGTTCGCGCCGGCTACGACCTGGACGCCGCGGCCATCCTGCTGTGCGAATCCG
ACGGCACGCCCGAAGAGGTGGCCGAAGAGATCGCACGGATGAGCGAGGTGCTGCGCGCCTCCGGCGCCACCCGCATCCAG
GTGTCGCAGAGCGAGGCCGAGCGGCTGAAATTCTGGAGCGGCCGCAAGAATGCCTTCCCGGCCGCCGGGCGCATCTCGCC
GGACTACTACTGCATGGACGGCACCATCCCGCGCAAGCACATCGGCACGCTGCTCAAGCGCATCCAGCAGATGGAGCAAA
ACTACGCGCTGCGCTGCATCAACGTCTTCCACGCCGGCGACGGCAACATGCACCCGCTGATCCTCTTCAACGGCGAAGAC
CAGGATGAATGGCACCGCGCCGAGCTGTTCGGCGCGGACATCCTCGAGACCTGCGTGGAGCTGGGCGGCACCGTCACCGG
CGAGCATGGCGTGGGGGTCGAGAAGCTCAATTCGATGTGCGTGCAGTTCTCGGCCGAGGAGCGCGATGCCTTCCTGCGCG
TCAAGGCCGCCTTCGATCCGGCGCGCCTGCTGAACCCAGACAAGGCCATCCCCACGCTCGCGCGCTGCGCCGAATACGGC
AAGCTGCACGTGCGCAACGGCCTGCTGCCGCACCCCGACCTGCCGCGCTTCTGA

Upstream 100 bases:

>100_bases
TCGCGGTAAAAGTCCTTAACACGCCGTGCGGCGCGGCGGCCCGGCTATAGTGCCTCGACACCGGCCACCGCCGCCGGACG
ACAACGATGAGAGACACCCC

Downstream 100 bases:

>100_bases
GGCCGCACGCATGACCGCACTCGACCCCGCGCTCGCGCGCCTGCGCGACGCCATCCTGCAGGCCGCCGCCGACCGCGCGC
CGCTCACCCTGCGCGGCGGC

Product: glycolate oxidase FAD-linked subunit oxidoreductase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNAPLPAAARDPSAVQAELIAALSPLMPADALLWEREDTTPYECDGLAAYRQVPLAVALPDSEAQVVEILRACHRLGVPV
VPRGAGTSLSGGAMPTPGGLVLSLAKFKRILKLDPHTRTAAVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVSE
NSGGVHCLKYGLTVHNVLRVRAVMMDGEVVEFGSEAPDAPGLDLLAAVIGSEGMLAVVTEVTVRLVPKPQLAQVIMASFD
DVEAGGNAVAEVIAAGIIPAGLEMMDKPATAAVEEFVRAGYDLDAAAILLCESDGTPEEVAEEIARMSEVLRASGATRIQ
VSQSEAERLKFWSGRKNAFPAAGRISPDYYCMDGTIPRKHIGTLLKRIQQMEQNYALRCINVFHAGDGNMHPLILFNGED
QDEWHRAELFGADILETCVELGGTVTGEHGVGVEKLNSMCVQFSAEERDAFLRVKAAFDPARLLNPDKAIPTLARCAEYG
KLHVRNGLLPHPDLPRF

Sequences:

>Translated_497_residues
MNAPLPAAARDPSAVQAELIAALSPLMPADALLWEREDTTPYECDGLAAYRQVPLAVALPDSEAQVVEILRACHRLGVPV
VPRGAGTSLSGGAMPTPGGLVLSLAKFKRILKLDPHTRTAAVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVSE
NSGGVHCLKYGLTVHNVLRVRAVMMDGEVVEFGSEAPDAPGLDLLAAVIGSEGMLAVVTEVTVRLVPKPQLAQVIMASFD
DVEAGGNAVAEVIAAGIIPAGLEMMDKPATAAVEEFVRAGYDLDAAAILLCESDGTPEEVAEEIARMSEVLRASGATRIQ
VSQSEAERLKFWSGRKNAFPAAGRISPDYYCMDGTIPRKHIGTLLKRIQQMEQNYALRCINVFHAGDGNMHPLILFNGED
QDEWHRAELFGADILETCVELGGTVTGEHGVGVEKLNSMCVQFSAEERDAFLRVKAAFDPARLLNPDKAIPTLARCAEYG
KLHVRNGLLPHPDLPRF
>Mature_497_residues
MNAPLPAAARDPSAVQAELIAALSPLMPADALLWEREDTTPYECDGLAAYRQVPLAVALPDSEAQVVEILRACHRLGVPV
VPRGAGTSLSGGAMPTPGGLVLSLAKFKRILKLDPHTRTAAVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVSE
NSGGVHCLKYGLTVHNVLRVRAVMMDGEVVEFGSEAPDAPGLDLLAAVIGSEGMLAVVTEVTVRLVPKPQLAQVIMASFD
DVEAGGNAVAEVIAAGIIPAGLEMMDKPATAAVEEFVRAGYDLDAAAILLCESDGTPEEVAEEIARMSEVLRASGATRIQ
VSQSEAERLKFWSGRKNAFPAAGRISPDYYCMDGTIPRKHIGTLLKRIQQMEQNYALRCINVFHAGDGNMHPLILFNGED
QDEWHRAELFGADILETCVELGGTVTGEHGVGVEKLNSMCVQFSAEERDAFLRVKAAFDPARLLNPDKAIPTLARCAEYG
KLHVRNGLLPHPDLPRF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=423, Percent_Identity=28.3687943262411, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI37595754, Length=446, Percent_Identity=27.5784753363229, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI119964728, Length=482, Percent_Identity=25.5186721991701, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI4501993, Length=222, Percent_Identity=27.4774774774775, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1789351, Length=486, Percent_Identity=59.4650205761317, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1787977, Length=503, Percent_Identity=22.2664015904573, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994907, Length=505, Percent_Identity=22.3762376237624, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71992373, Length=427, Percent_Identity=27.1662763466042, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71992381, Length=427, Percent_Identity=25.2927400468384, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=387, Percent_Identity=25.0645994832041, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17534361, Length=490, Percent_Identity=25.9183673469388, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=286, Percent_Identity=25.1748251748252, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320027, Length=434, Percent_Identity=26.4976958525346, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320023, Length=491, Percent_Identity=26.8839103869654, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320764, Length=499, Percent_Identity=24.248496993988, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18921117, Length=467, Percent_Identity=28.4796573875803, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24639277, Length=467, Percent_Identity=28.4796573875803, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24639275, Length=467, Percent_Identity=28.4796573875803, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24653753, Length=223, Percent_Identity=29.1479820627803, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53000; Mature: 53000

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLPAAARDPSAVQAELIAALSPLMPADALLWEREDTTPYECDGLAAYRQVPLAVALP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCCHHHHHHCCEEEECC
DSEAQVVEILRACHRLGVPVVPRGAGTSLSGGAMPTPGGLVLSLAKFKRILKLDPHTRTA
CCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCE
AVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVSENSGGVHCLKYGLTVHNVLRV
ECCCCHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECHHHHHHHHH
RAVMMDGEVVEFGSEAPDAPGLDLLAAVIGSEGMLAVVTEVTVRLVPKPQLAQVIMASFD
HHHHCCCCCEECCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHEECCCHHHHHHHHHHCC
DVEAGGNAVAEVIAAGIIPAGLEMMDKPATAAVEEFVRAGYDLDAAAILLCESDGTPEEV
CCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHH
AEEIARMSEVLRASGATRIQVSQSEAERLKFWSGRKNAFPAAGRISPDYYCMDGTIPRKH
HHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHH
IGTLLKRIQQMEQNYALRCINVFHAGDGNMHPLILFNGEDQDEWHRAELFGADILETCVE
HHHHHHHHHHHHHHHHEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LGGTVTGEHGVGVEKLNSMCVQFSAEERDAFLRVKAAFDPARLLNPDKAIPTLARCAEYG
HCCEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHHC
KLHVRNGLLPHPDLPRF
CEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNAPLPAAARDPSAVQAELIAALSPLMPADALLWEREDTTPYECDGLAAYRQVPLAVALP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCCHHHHHHCCEEEECC
DSEAQVVEILRACHRLGVPVVPRGAGTSLSGGAMPTPGGLVLSLAKFKRILKLDPHTRTA
CCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCE
AVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVSENSGGVHCLKYGLTVHNVLRV
ECCCCHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECHHHHHHHHH
RAVMMDGEVVEFGSEAPDAPGLDLLAAVIGSEGMLAVVTEVTVRLVPKPQLAQVIMASFD
HHHHCCCCCEECCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHEECCCHHHHHHHHHHCC
DVEAGGNAVAEVIAAGIIPAGLEMMDKPATAAVEEFVRAGYDLDAAAILLCESDGTPEEV
CCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHH
AEEIARMSEVLRASGATRIQVSQSEAERLKFWSGRKNAFPAAGRISPDYYCMDGTIPRKH
HHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHH
IGTLLKRIQQMEQNYALRCINVFHAGDGNMHPLILFNGEDQDEWHRAELFGADILETCVE
HHHHHHHHHHHHHHHHEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LGGTVTGEHGVGVEKLNSMCVQFSAEERDAFLRVKAAFDPARLLNPDKAIPTLARCAEYG
HCCEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHHC
KLHVRNGLLPHPDLPRF
CEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]