Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 17547384

GI number: 17547384

Start: 2874670

End: 2875560

Strand: Direct

Name: gcvA [H]

Synonym: RSc2665

Alternate gene names: 17547384

Gene position: 2874670-2875560 (Clockwise)

Preceding gene: 17547383

Following gene: 17547385

Centisome position: 77.35

GC content: 69.47

Gene sequence:

>891_bases
ATGGCTTCCGTCTTCAAGCGCGTCCCGCCGCTGCATCTGCTGATCGCCTTCGAAGCGGGCGCGCGCCACGCCAGCTTTGC
GCGCGCGGCCGAGGAGCTCTCTGTCACGCCCAGCGCGGTCTCCCACCGCATCAAGAACCTCGAGGCACTGTGGGGCGAAG
ACCTGTTCGTGCGCACCGGCACCACGCTGCGGCTGACCGCGGCCGGCACGCGCTACCTGCGCAGCGTGCAGGATGCGCTC
AAGTCCCTGCACGAGCTGGCGCGGCCGGAGTTCAGCAAGCAGCGCGCGCGGCTGCGCGTGGCGATCCCGCCCACCTTCGG
CCGCCAGCACCTGGTGCCGCGCCTGCCGGAGTTCCAGGCGCTGTATCCGCACATCGACCTGGAGCTGCATCTCGCCATCC
CCTTCCTCGACGTGAAGGCCGAGGATACCGACGTCGAGATCCGCTACGGCACCGGCCGCTACCCGGACCTGAAGACCACG
CTGCTGCTCAACGAGCCGGTCTTCCCCGCCTGCGGGCGCGGCTACTTCGAGCAGATCGGCGGCAACACCATCGCCGAGCC
GGCGCAGTTGCTGAACCTGAACCTGCTGCGCAGCCCGCTGGAGCCATGGCGCCCGTGGTTCGAGGCGGCCGGGCTGGACG
CGGCGGAACCCTCCACCGGCTCGCTCTTCAACGACATCGGGCTGATGCTGGAAGCAGTCGCCTCCAACCAGGGCGTGGCG
CTGGTGCGCCAGCGCATGGCGCGCAACTGGCTGGCCTCGGGGCAGATGGTGCGACTGCTGGACATCGAATCCGCCTCGCC
GCATGCTTACTACATCGTCCAGCGCGAGCAGGCGCCGCTCGAGCCGGAAGCGCGGCAATTCGTCGACTGGCTGCTCGGGC
TGGATTGGTAG

Upstream 100 bases:

>100_bases
GGCGACGATGCGATCGACCTGATGCGCAGCGTCAAGCAGGCGCTGGACCCGAAGCACATCCTGAACCCCGGCAAGATCTT
CCGGCTGTAGGCGCCAGCGC

Downstream 100 bases:

>100_bases
GCCGCGGGGCCCGGCCACCGCGCGATGCATCCCGATGAATCCGATTGGCCGGCATGGCGAGCCGAATTCATCGCGGTAAA
AGTCCTTAACACGCCGTGCG

Product: transcription regulator protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MASVFKRVPPLHLLIAFEAGARHASFARAAEELSVTPSAVSHRIKNLEALWGEDLFVRTGTTLRLTAAGTRYLRSVQDAL
KSLHELARPEFSKQRARLRVAIPPTFGRQHLVPRLPEFQALYPHIDLELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTT
LLLNEPVFPACGRGYFEQIGGNTIAEPAQLLNLNLLRSPLEPWRPWFEAAGLDAAEPSTGSLFNDIGLMLEAVASNQGVA
LVRQRMARNWLASGQMVRLLDIESASPHAYYIVQREQAPLEPEARQFVDWLLGLDW

Sequences:

>Translated_296_residues
MASVFKRVPPLHLLIAFEAGARHASFARAAEELSVTPSAVSHRIKNLEALWGEDLFVRTGTTLRLTAAGTRYLRSVQDAL
KSLHELARPEFSKQRARLRVAIPPTFGRQHLVPRLPEFQALYPHIDLELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTT
LLLNEPVFPACGRGYFEQIGGNTIAEPAQLLNLNLLRSPLEPWRPWFEAAGLDAAEPSTGSLFNDIGLMLEAVASNQGVA
LVRQRMARNWLASGQMVRLLDIESASPHAYYIVQREQAPLEPEARQFVDWLLGLDW
>Mature_295_residues
ASVFKRVPPLHLLIAFEAGARHASFARAAEELSVTPSAVSHRIKNLEALWGEDLFVRTGTTLRLTAAGTRYLRSVQDALK
SLHELARPEFSKQRARLRVAIPPTFGRQHLVPRLPEFQALYPHIDLELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTTL
LLNEPVFPACGRGYFEQIGGNTIAEPAQLLNLNLLRSPLEPWRPWFEAAGLDAAEPSTGSLFNDIGLMLEAVASNQGVAL
VRQRMARNWLASGQMVRLLDIESASPHAYYIVQREQAPLEPEARQFVDWLLGLDW

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=33.7931034482759, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=30.2083333333333, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788706, Length=286, Percent_Identity=29.3706293706294, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=27.6450511945392, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=164, Percent_Identity=34.7560975609756, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=27.4914089347079, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=241, Percent_Identity=28.6307053941909, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=25.3521126760563, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87081978, Length=273, Percent_Identity=27.8388278388278, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33175; Mature: 33044

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVFKRVPPLHLLIAFEAGARHASFARAAEELSVTPSAVSHRIKNLEALWGEDLFVRTG
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECC
TTLRLTAAGTRYLRSVQDALKSLHELARPEFSKQRARLRVAIPPTFGRQHLVPRLPEFQA
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEEEEEECCCCCCCCCCCCCCCHHH
LYPHIDLELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTTLLLNEPVFPACGRGYFEQIG
HCCCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHC
GNTIAEPAQLLNLNLLRSPLEPWRPWFEAAGLDAAEPSTGSLFNDIGLMLEAVASNQGVA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
LVRQRMARNWLASGQMVRLLDIESASPHAYYIVQREQAPLEPEARQFVDWLLGLDW
HHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASVFKRVPPLHLLIAFEAGARHASFARAAEELSVTPSAVSHRIKNLEALWGEDLFVRTG
CCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECC
TTLRLTAAGTRYLRSVQDALKSLHELARPEFSKQRARLRVAIPPTFGRQHLVPRLPEFQA
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEEEEEECCCCCCCCCCCCCCCHHH
LYPHIDLELHLAIPFLDVKAEDTDVEIRYGTGRYPDLKTTLLLNEPVFPACGRGYFEQIG
HCCCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHC
GNTIAEPAQLLNLNLLRSPLEPWRPWFEAAGLDAAEPSTGSLFNDIGLMLEAVASNQGVA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
LVRQRMARNWLASGQMVRLLDIESASPHAYYIVQREQAPLEPEARQFVDWLLGLDW
HHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]