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The map label for this gene is panB

Identifier: 17547349

GI number: 17547349

Start: 2832620

End: 2833444

Strand: Direct

Name: panB

Synonym: RSc2630

Alternate gene names: 17547349

Gene position: 2832620-2833444 (Clockwise)

Preceding gene: 17547348

Following gene: 17547350

Centisome position: 76.22

GC content: 69.58

Gene sequence:

>825_bases
ATGAGCAGCTATCTCCAGGAATCCACCCGCAAGGCCGTGACCGTCACCTCGCTGCAGGGCATGCGCGCGGCGGGCGAGCG
CATCGCCATGCTGACGGCCTACGATGCCAGCTTCGCCGCCCTGATGGAGCGCAGCGGCGTGGACGTGGTGCTGGTCGGCG
ATTCGCTGGGCAACGTGGTGCAGGGCCAGAAGACCACGCTGCCCGTCACGCTCGACCACATCGTCTACCACACCGAATGC
GTGGCGCGCGGCACGACCAAGGCGCTGCTCATGTCGGATCTGCCCTTCGGCACCTACGGCACGCCCGAGCAAGCCTTCCA
CAGCGCCGTGCGCGTGATGCAGGCCGGCGCGCAGATGGTCAAGCTCGAAGGCGGCGAGTGGCTGGCGCCCACCCTCCGTT
TCCTGGTCGAACGCAGCATTCCGGTGTGCGCGCACATCGGCCTGACGCCGCAATCGGTGCATGCCTTCGGCGGCTTCAAG
GTCCAGGGCCGGGGCGACGAGGCCGCCGCGCAGCTCAAGGCCGATGCGCTGGCCGTGCAGGACGCCGGCGCGCAGATGGT
CCTCATGGAAGCCATTCCCGCCACGCTGGCCGGCGAGGTGACGCGCCTGCTGGCGGTCCCCACCATCGGCATCGGCGCCG
GGCCCGAGTGCTCGGGCCAGGTGCTGGTGATGCACGACATGCTGGGCGTGTTCCCCGGCCACCGGCCCAAGTTCGTGCGC
AACTTCATGGACGGCCTGACCACCATCGACGCCGCCGTCACGGCCTATGTGACGGCGGTCAAGGACGGCACCTTCCCCGG
CCCGGAGCACAGCTTCACCGCCTGA

Upstream 100 bases:

>100_bases
CCATGCGCGGGCCCAAGGAATTCATCAAGCTCGCGACCCGGTAGCAGGTCCATCGCATGGCCTGCCTGGAAGGTCGCGGC
GCTCAACCTGATCCCAGTCC

Downstream 100 bases:

>100_bases
GCGCGCCGCGCATGGACATCTGGTCGGCCACCGTCGAGGCGTTCGCCCTGCTCACCCGCGGCGACGCGGCGCTCTGGGTG
ATCGTCTGGACCTCGCTGAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSSYLQESTRKAVTVTSLQGMRAAGERIAMLTAYDASFAALMERSGVDVVLVGDSLGNVVQGQKTTLPVTLDHIVYHTEC
VARGTTKALLMSDLPFGTYGTPEQAFHSAVRVMQAGAQMVKLEGGEWLAPTLRFLVERSIPVCAHIGLTPQSVHAFGGFK
VQGRGDEAAAQLKADALAVQDAGAQMVLMEAIPATLAGEVTRLLAVPTIGIGAGPECSGQVLVMHDMLGVFPGHRPKFVR
NFMDGLTTIDAAVTAYVTAVKDGTFPGPEHSFTA

Sequences:

>Translated_274_residues
MSSYLQESTRKAVTVTSLQGMRAAGERIAMLTAYDASFAALMERSGVDVVLVGDSLGNVVQGQKTTLPVTLDHIVYHTEC
VARGTTKALLMSDLPFGTYGTPEQAFHSAVRVMQAGAQMVKLEGGEWLAPTLRFLVERSIPVCAHIGLTPQSVHAFGGFK
VQGRGDEAAAQLKADALAVQDAGAQMVLMEAIPATLAGEVTRLLAVPTIGIGAGPECSGQVLVMHDMLGVFPGHRPKFVR
NFMDGLTTIDAAVTAYVTAVKDGTFPGPEHSFTA
>Mature_273_residues
SSYLQESTRKAVTVTSLQGMRAAGERIAMLTAYDASFAALMERSGVDVVLVGDSLGNVVQGQKTTLPVTLDHIVYHTECV
ARGTTKALLMSDLPFGTYGTPEQAFHSAVRVMQAGAQMVKLEGGEWLAPTLRFLVERSIPVCAHIGLTPQSVHAFGGFKV
QGRGDEAAAQLKADALAVQDAGAQMVLMEAIPATLAGEVTRLLAVPTIGIGAGPECSGQVLVMHDMLGVFPGHRPKFVRN
FMDGLTTIDAAVTAYVTAVKDGTFPGPEHSFTA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=55.3435114503817, Blast_Score=301, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6319653, Length=269, Percent_Identity=35.6877323420074, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_RALSO (Q8XW45)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520751.1
- ProteinModelPortal:   Q8XW45
- SMR:   Q8XW45
- GeneID:   1221477
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2630
- NMPDR:   fig|267608.1.peg.2630
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   RSOL267608:RSC2630-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28888; Mature: 28756

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: ACT_SITE 190-190 BINDING 93-93 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYLQESTRKAVTVTSLQGMRAAGERIAMLTAYDASFAALMERSGVDVVLVGDSLGNVV
CCHHHHHHHHCEEEEEHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCEEEEECCCCCCHH
QGQKTTLPVTLDHIVYHTECVARGTTKALLMSDLPFGTYGTPEQAFHSAVRVMQAGAQMV
CCCCCEECEEHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEE
KLEGGEWLAPTLRFLVERSIPVCAHIGLTPQSVHAFGGFKVQGRGDEAAAQLKADALAVQ
EECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHEEE
DAGAQMVLMEAIPATLAGEVTRLLAVPTIGIGAGPECSGQVLVMHDMLGVFPGHRPKFVR
CCCCEEEEHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHH
NFMDGLTTIDAAVTAYVTAVKDGTFPGPEHSFTA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSYLQESTRKAVTVTSLQGMRAAGERIAMLTAYDASFAALMERSGVDVVLVGDSLGNVV
CHHHHHHHHCEEEEEHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCEEEEECCCCCCHH
QGQKTTLPVTLDHIVYHTECVARGTTKALLMSDLPFGTYGTPEQAFHSAVRVMQAGAQMV
CCCCCEECEEHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEE
KLEGGEWLAPTLRFLVERSIPVCAHIGLTPQSVHAFGGFKVQGRGDEAAAQLKADALAVQ
EECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHEEE
DAGAQMVLMEAIPATLAGEVTRLLAVPTIGIGAGPECSGQVLVMHDMLGVFPGHRPKFVR
CCCCEEEEHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHH
NFMDGLTTIDAAVTAYVTAVKDGTFPGPEHSFTA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852