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The map label for this gene is pcaJ [H]

Identifier: 17546972

GI number: 17546972

Start: 2445855

End: 2446517

Strand: Reverse

Name: pcaJ [H]

Synonym: RSc2253

Alternate gene names: 17546972

Gene position: 2446517-2445855 (Counterclockwise)

Preceding gene: 17546973

Following gene: 17546971

Centisome position: 65.83

GC content: 69.38

Gene sequence:

>663_bases
ATGAACGCCGAAGCCACCGCCAAGATCCAGCGCTGGACGCGCGACCAGATCGCCGCCCGCGTGGCGCGCGACATCCCCGA
CGGCTCGGTCGTCAACCTCGGTATCGGCCTGCCGACGCTGGTGGCCAACCAGCTGCCGGCCGACCGCGAGATCCTGCTGC
ACAGCGAGAACGGCCTGCTCGGCATGGGCCCGGCGCCCGCGCCCGGCGCGGAAGACCCCGACCTCATCAACGCCGGCAAG
CAGCCGGTGACCATCAAGCCGGGCGGGGCTTACTTCCACCACGCCGATTCGTTTGCCATGATGCGGGGTGGCCATCTCGA
CTACTGCGTGCTCGGCGCCTTCCAGGTGTCGGCCGCCGGCGACCTGGCCAACTGGCACACCGGCGCGCCGGACGCGATTC
CCGCCGTGGGCGGGGCGATGGACCTGGCCATCGGCGCCAAGCAGGTCTTCGTGATGATGGAGCACCAGACCAAGCAGGGC
GACAGCAAGATCGTGCCCGCGTGCACCTACCCGCTGACAGGCATCGGCTGCGTGGATACCATCTACACCGATCTCGCCGT
GATCGACGTCACGCCCGGCGGGCTGATCGTGCGCGAGATGGCGGATGGCCTCGATTTCGAGACGCTGCAGACGCTGACGG
CCGTGCCGCTGCGTCGCGCTTGA

Upstream 100 bases:

>100_bases
TCGAGCTGGGCGCGCTGGACCCGGAGCACATCGTCACGCCGGGCATCTTCGTCAAGCGCATCGTGCCGGTCGGCGCCGCC
GCGCAAACCAGGGAGGCCGC

Downstream 100 bases:

>100_bases
CGGTTCAGGACACAGGACAATCATGACGGAAGCCTTTATCTGCGACGCCATCCGCACGCCCATCGGCCGCTACGGCGGCA
GCCTGTCCGCCGTGCGCGCG

Product: 3-oxoadipate CoA-transferase subunit B protein

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNAEATAKIQRWTRDQIAARVARDIPDGSVVNLGIGLPTLVANQLPADREILLHSENGLLGMGPAPAPGAEDPDLINAGK
QPVTIKPGGAYFHHADSFAMMRGGHLDYCVLGAFQVSAAGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHQTKQG
DSKIVPACTYPLTGIGCVDTIYTDLAVIDVTPGGLIVREMADGLDFETLQTLTAVPLRRA

Sequences:

>Translated_220_residues
MNAEATAKIQRWTRDQIAARVARDIPDGSVVNLGIGLPTLVANQLPADREILLHSENGLLGMGPAPAPGAEDPDLINAGK
QPVTIKPGGAYFHHADSFAMMRGGHLDYCVLGAFQVSAAGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHQTKQG
DSKIVPACTYPLTGIGCVDTIYTDLAVIDVTPGGLIVREMADGLDFETLQTLTAVPLRRA
>Mature_220_residues
MNAEATAKIQRWTRDQIAARVARDIPDGSVVNLGIGLPTLVANQLPADREILLHSENGLLGMGPAPAPGAEDPDLINAGK
QPVTIKPGGAYFHHADSFAMMRGGHLDYCVLGAFQVSAAGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHQTKQG
DSKIVPACTYPLTGIGCVDTIYTDLAVIDVTPGGLIVREMADGLDFETLQTLTAVPLRRA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=202, Percent_Identity=48.019801980198, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI4557817, Length=202, Percent_Identity=47.5247524752475, Blast_Score=174, Evalue=6e-44,
Organism=Escherichia coli, GI1788552, Length=200, Percent_Identity=48, Blast_Score=183, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17531517, Length=209, Percent_Identity=47.3684210526316, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI21358325, Length=192, Percent_Identity=49.4791666666667, Blast_Score=183, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24655794, Length=192, Percent_Identity=49.4791666666667, Blast_Score=183, Evalue=9e-47,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23077; Mature: 23077

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEATAKIQRWTRDQIAARVARDIPDGSVVNLGIGLPTLVANQLPADREILLHSENGLL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCEEEEECCCCEE
GMGPAPAPGAEDPDLINAGKQPVTIKPGGAYFHHADSFAMMRGGHLDYCVLGAFQVSAAG
ECCCCCCCCCCCCHHHCCCCCCEEECCCCCEEEECCCCHHHCCCCCCEEEEEEEEECCCC
DLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHQTKQGDSKIVPACTYPLTGIGCVDT
CCCCCCCCCCCCHHHCCCHHHHHHCCEEEEEEEECCCCCCCCEECEEECCCCCCCHHHHH
IYTDLAVIDVTPGGLIVREMADGLDFETLQTLTAVPLRRA
HHHCEEEEEECCCCEEHHHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNAEATAKIQRWTRDQIAARVARDIPDGSVVNLGIGLPTLVANQLPADREILLHSENGLL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCEEEEECCCCEE
GMGPAPAPGAEDPDLINAGKQPVTIKPGGAYFHHADSFAMMRGGHLDYCVLGAFQVSAAG
ECCCCCCCCCCCCHHHCCCCCCEEECCCCCEEEECCCCHHHCCCCCCEEEEEEEEECCCC
DLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHQTKQGDSKIVPACTYPLTGIGCVDT
CCCCCCCCCCCCHHHCCCHHHHHHCCEEEEEEEECCCCCCCCEECEEECCCCCCCHHHHH
IYTDLAVIDVTPGGLIVREMADGLDFETLQTLTAVPLRRA
HHHCEEEEEECCCCEEHHHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]