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The map label for this gene is pacF [H]

Identifier: 17546971

GI number: 17546971

Start: 2444630

End: 2445832

Strand: Reverse

Name: pacF [H]

Synonym: RSc2252

Alternate gene names: 17546971

Gene position: 2445832-2444630 (Counterclockwise)

Preceding gene: 17546972

Following gene: 17546970

Centisome position: 65.81

GC content: 71.82

Gene sequence:

>1203_bases
ATGACGGAAGCCTTTATCTGCGACGCCATCCGCACGCCCATCGGCCGCTACGGCGGCAGCCTGTCCGCCGTGCGCGCGGA
CGACCTCGGCGCGGTGCCCCTCAAGGCGCTGATGGCGCGCAACCCGCGGGTCGACTGGTCGGCCATTGACGACGTGATCT
TCGGCTGCGCCAACCAGGCCGGCGAGGACAACCGCAACGTGGCGCGCATGTCGGCGCTGCTGGCCGGACTGCCCGACAGC
GTGCCGGGCTCGACCATCAACCGCCTGTGCGGCTCGGGCATGGACGCCACCGGCACCGCCGCGCGCGCCATCCGCGCCGG
CGAAACCGCGCTGATGATCGCCGGCGGCGTGGAGAGCATGAGCCGCGCGCCCTTCGTGATGGGCAAGGCCGCCAGCGCCT
TCTCGCGCGACGCCGCCATGTACGACACCACCATCGGCTGGCGCTTCGTCAATCCGCTGATGCAGGCGCAGTACGGCGTC
GACTCGATGCCCGAGACCGCCGAGAACGTCGCCGCCGACTACCGGGTCAGCCGCGAAGACCAGGATCGCTTCGCGCTGCG
CAGCCAGGCCAGCGCCGCGCGCGCGCAGGCCGACGGCACGCTGGCGCAGGAAATCACGCCGGTGACGATCGCGCAGAAGA
AGGGCGATCCGATCGTCGTAGACCGCGACGAGCATCCGCGCGCGACCACCCTGGAAGCGCTCGCCAGGCTCAAGGGCGTG
GTGCGCCCGGCCGGCACCGTCACGGCCGGCAATGCCTCGGGCGTGAACGACGGCGCCTGCGCGCTGCTGCTCGCCAACGA
AGAGGCCGCCAAGCGCTTCGGCCTGACACCGCGCGCGCGCATCATCGGCATGGCCACCGCCGGCGTGCCGCCGCGCGTGA
TGGGCATCGGCCCGGCGCCGGCCTCGCAGAAGGTGCTCGGGCAGCTTGGCCTGACGATCGACCAGATGGATGTGATCGAG
CTGAACGAAGCGTTTGCCGCGCAAGGCCTGGCGGTGACGCGCCAGCTCGGCCTGGCCGACGACGATGCGCGCGTCAACCC
GAACGGCGGGGCCATCGCGCTGGGCCACCCGCTCGGCATGAGCGGCGCGCGCCTGGTGACCACGGCGATGTACCAACTGC
AGCGCACCGGCGGCCGCTATGCGCTGTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGCTGGTGATCGAACGCGTG
TGA

Upstream 100 bases:

>100_bases
ATCGTGCGCGAGATGGCGGATGGCCTCGATTTCGAGACGCTGCAGACGCTGACGGCCGTGCCGCTGCGTCGCGCTTGACG
GTTCAGGACACAGGACAATC

Downstream 100 bases:

>100_bases
ACACGGACGCGCCATGATTACCGCACTGCTCGATCGTACCTTCTCCACACCGGCCATGCTGGCGGTGTGGTCCGACGCGG
CCACCGTGCGCGCGATGCTC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPRVDWSAIDDVIFGCANQAGEDNRNVARMSALLAGLPDS
VPGSTINRLCGSGMDATGTAARAIRAGETALMIAGGVESMSRAPFVMGKAASAFSRDAAMYDTTIGWRFVNPLMQAQYGV
DSMPETAENVAADYRVSREDQDRFALRSQASAARAQADGTLAQEITPVTIAQKKGDPIVVDRDEHPRATTLEALARLKGV
VRPAGTVTAGNASGVNDGACALLLANEEAAKRFGLTPRARIIGMATAGVPPRVMGIGPAPASQKVLGQLGLTIDQMDVIE
LNEAFAAQGLAVTRQLGLADDDARVNPNGGAIALGHPLGMSGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV

Sequences:

>Translated_400_residues
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPRVDWSAIDDVIFGCANQAGEDNRNVARMSALLAGLPDS
VPGSTINRLCGSGMDATGTAARAIRAGETALMIAGGVESMSRAPFVMGKAASAFSRDAAMYDTTIGWRFVNPLMQAQYGV
DSMPETAENVAADYRVSREDQDRFALRSQASAARAQADGTLAQEITPVTIAQKKGDPIVVDRDEHPRATTLEALARLKGV
VRPAGTVTAGNASGVNDGACALLLANEEAAKRFGLTPRARIIGMATAGVPPRVMGIGPAPASQKVLGQLGLTIDQMDVIE
LNEAFAAQGLAVTRQLGLADDDARVNPNGGAIALGHPLGMSGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV
>Mature_399_residues
TEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPRVDWSAIDDVIFGCANQAGEDNRNVARMSALLAGLPDSV
PGSTINRLCGSGMDATGTAARAIRAGETALMIAGGVESMSRAPFVMGKAASAFSRDAAMYDTTIGWRFVNPLMQAQYGVD
SMPETAENVAADYRVSREDQDRFALRSQASAARAQADGTLAQEITPVTIAQKKGDPIVVDRDEHPRATTLEALARLKGVV
RPAGTVTAGNASGVNDGACALLLANEEAAKRFGLTPRARIIGMATAGVPPRVMGIGPAPASQKVLGQLGLTIDQMDVIEL
NEAFAAQGLAVTRQLGLADDDARVNPNGGAIALGHPLGMSGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=42.0654911838791, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=39.2947103274559, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=36.1596009975062, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=40.4522613065327, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=33.1764705882353, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=30.7106598984772, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=70.573566084788, Blast_Score=569, Evalue=1e-164,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=47.1464019851117, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=44.1975308641975, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=43.142144638404, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=31.6784869976359, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=43.640897755611, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=34.9009900990099, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.743842364532, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=436, Percent_Identity=32.5688073394495, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=30.8641975308642, Blast_Score=158, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=39.3034825870647, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=35.9223300970874, Blast_Score=216, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.8902743142145, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=39.9014778325123, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=34.5679012345679, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.8396226415094, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41638; Mature: 41507

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPRVDWSAIDDVIFGCANQA
CCCHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMSALLAGLPDSVPGSTINRLCGSGMDATGTAARAIRAGETALMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKAASAFSRDAAMYDTTIGWRFVNPLMQAQYGVDSMPETAENVAADYRVSRED
HCCCEEECCHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
QDRFALRSQASAARAQADGTLAQEITPVTIAQKKGDPIVVDRDEHPRATTLEALARLKGV
HHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
VRPAGTVTAGNASGVNDGACALLLANEEAAKRFGLTPRARIIGMATAGVPPRVMGIGPAP
CCCCCEEECCCCCCCCCCEEEEEEECHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCC
ASQKVLGQLGLTIDQMDVIELNEAFAAQGLAVTRQLGLADDDARVNPNGGAIALGHPLGM
HHHHHHHHHCCEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
TEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPRVDWSAIDDVIFGCANQA
CCHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMSALLAGLPDSVPGSTINRLCGSGMDATGTAARAIRAGETALMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKAASAFSRDAAMYDTTIGWRFVNPLMQAQYGVDSMPETAENVAADYRVSRED
HCCCEEECCHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
QDRFALRSQASAARAQADGTLAQEITPVTIAQKKGDPIVVDRDEHPRATTLEALARLKGV
HHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
VRPAGTVTAGNASGVNDGACALLLANEEAAKRFGLTPRARIIGMATAGVPPRVMGIGPAP
CCCCCEEECCCCCCCCCCEEEEEEECHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCC
ASQKVLGQLGLTIDQMDVIELNEAFAAQGLAVTRQLGLADDDARVNPNGGAIALGHPLGM
HHHHHHHHHCCEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]